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Genetic and biological signature of Potato Virus X circulating in Kazakhstan | ||
Caspian Journal of Environmental Sciences | ||
دوره 21، شماره 5، اسفند 2023، صفحه 1151-1157 اصل مقاله (1.04 M) | ||
نوع مقاله: Research Paper | ||
شناسه دیجیتال (DOI): 10.22124/cjes.2023.7403 | ||
نویسندگان | ||
Moldir Azhimakhan1؛ Bibigul Beisembina1؛ Anastasiya Kapytina2؛ Nazym Kerimbek2؛ Dilyara Gritsenko2؛ Dianqiu Lv3؛ Hu Baigeng4؛ Vadim Khassanov* 1 | ||
1Saken Seifullin Kazakh Agrotechnical Research University, Astana, Kazakhstan | ||
2Institute of Plant Biology and Biotechnology, Almaty, Kazakhstan | ||
3Southwest University, Chongqing, China | ||
4Leling Xisen Potato Industry Group Company Ltd, Leling, China | ||
چکیده | ||
This research studied ten isolates of potato virus X (PVX) isolated from potato cultivars growing in Kazakhstan (KZ), Belarus (BY) and China (CN). Additionally, the potato samples were screened for Potato virus Y (PVY), Potato virus S (PVS), and Potato leafroll virus (PLRV) using multiplex reverse transcription-polymerase chain reaction (RT-PCR). PVX was isolated from the potato varieties ‘Tamasha’, ‘Red Scarlet’, ‘Gala variety’, ‘Krone’, ‘Juwel’, ‘Gala’, and ‘b2-7’. The identified isolates of PVX were sequenced and used to construct a phylogenetic tree, which demonstrated that KZ and BY isolates form clusters closely related to isolates from Asia and Europe, however, CN isolate formed a separate clade. KZ5 and KZ7 PVX isolates showed local symptoms of mosaic and light green speckled spots on the upper leaves of Datura stramonium in pathogenicity test. | ||
کلیدواژهها | ||
Potato؛ Potato Virus X؛ isolates؛ ELISA؛ PCR؛ Targeted sequencing؛ Indicator plants | ||
مراجع | ||
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