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بهینهسازی پایداری اسپورهای دو جدایه از قارچ بیمارگر حشرات، Beauveria bassiana، در برابر تابش اشعه فرابنفش با استفاده از فرمولاسیون نانوکامپوزیت | ||
تحقیقات آفات گیاهی | ||
دوره 13، شماره 3، آذر 1402، صفحه 27-42 اصل مقاله (1.55 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22124/iprj.2023.25524.1534 | ||
نویسندگان | ||
مائده رئوفی1؛ سید علی صفوی* 1؛ مهدی محمودیان2 | ||
1گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران | ||
2گروه نانوفناوری، دانشکده شیمی، دانشگاه ارومیه، ارومیه، ایران | ||
چکیده | ||
قارچ بیمارگر حشراتBeauveria bassiana یک عامل کنترل بیولوژیک مهم و جهانی است که برای کنترل بسیاری از آفات مهم مثل شته مومیکلم Brevicoryne brassicae استفاده میشود. محدودیت عمده استفاده از این قارچ، پایداری محیطی برای شروع همهگیری در جمعیتهای میزبان حساس است. در پژوهش حاضر، کشندگی دو جدایه از قارچ بیمارگر حشرات B. bassiana روی حشرات کامل و پورههای سن دوم شته مومیکلم و پایداری قارچ بیمارگر حشرات و فرمولاسیون نانوکامپوزیت آن در برابر اشعه UV-C بررسی شد. مقدار LC50 جدایه WE209 در مادههای بالغ شته مومیکلم برابر با 105×5/2 و در جدایه WE277، 104×8/9 اسپور بر میلیلیتر به دست آمد. مقادیر LC50 قارچ روی پوره سن دوم شته بهترتیب 105×4/6 و 105×2/3 اسپور بر میلیلیتر بوده است. درصد جوانهزنی اسپورهای قارچ هنگامی که 2 و 3 ساعت در برابر اشعه فرابنفش قرار داشتند، در جدایه WE209 در هر دو زمان برابر با صفر و در جدایه WE277 این مقدار بهترتیب 32% و 6% بود. در حالی که در فرمولاسیون نانوکامپورزیت مبتنی بر قارچ این مقادیر بهترتیب برای جدایه WE209، 37 % و 12% و برای جدایه WE277، 65 % و 38% بودند. این نتایج نشان داد که فرمولاسیون نانوکامپوزیت قارچ بیمارگر B. bassiana مبتنی بر مونت موریلونیت/ثعلب با پوشش اسپورهای قارچ از آن در برابر اشعه فرابنفش محافظت کرده و پایداری آن را افزایش میدهد. | ||
کلیدواژهها | ||
اشعه UV-C؛ شته مومیکلم؛ قارچ بیمارگر حشرات؛ کنترل بیولوژیک؛ نانوکامپوزیت | ||
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