The effect of probiotic bacteria Bacillus subtilis  and Lactobacillus plantarum  on fungal control and egg hatching performance of rainbow trout (Oncorhynchus mykiss)

Rahmati, Omid; Aeyaeinejad, Saeid; Abromand, Ali; Kazemi, Esmaeil

doi:10.22124/japb.2022.22297.1465

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	The effect of probiotic bacteria Bacillus subtilis and Lactobacillus plantarum on fungal control and egg hatching performance of rainbow trout (Oncorhynchus mykiss)
فیزیولوژی و بیوتکنولوژی آبزیان
Volume 11, Issue 1, June 2023, Pages 105-125 PDF (1.01 M)
Document Type: Research Paper
DOI: 10.22124/japb.2022.22297.1465
Authors
Omid Rahmati¹; Saeid Aeyaeinejad^* ¹; Ali Abromand¹; Esmaeil Kazemi²
¹Associate Professor in Department of Fisheries, Faculty of Natural Resources, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran
²Ph.D. in Fisheries, Fisheries Research Institute of Iran, Yasouj Shahid Motahari Clod-water Fish Genetics and Breeding Research Center, Fisheries Research Institute of Iran, Yasouj, Iran
Abstract
In the fish breeding industry, many disinfectants are used to prevent fungal contamination of eggs during the incubation period, including formalin, hydrogen peroxide, potassium permanganate, etc. The purpose of this research is to evaluate the effect of probiotic bacteria Bacillus subtilis 1 and 2 grams and Lactobacillus plantarum 1 and 2 grams (adding to water) in controlling the fungal contamination of rainbow trout eggs during the incubation stage and comparing its effects with formalin and It was distilled water. To conduct this study, 18 Californian troughs were used in 6 treatments and three repetitions, and 1850 fertilized rainbow trout eggs were transferred to each trough. After preparing the trays and distributing the eggs, a separate serum was used for each treatment to disinfect the eggs. The duration of complete emptying of each serum was 50 minutes. The results showed that in the treatments containing formalin (10±1) followed by Lactobacillus 2 grams (15.33±3.21), the lowest rate of fungal infection of eggs was observed, which showed a significant difference with other treatments. P>0.05). The highest rate of eye opening (84.67±0.58) and egg opening (81±1) was observed in formalin treatment, which was significantly different from other treatments (P<0.05)
Keywords
Rainbow Trout Eggs; Saprolegnia; Probiotics; Fungal Infection
Main Subjects
Aquatic Physiology and Biotechnology

References
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The effect of probiotic bacteria Bacillus subtilis and Lactobacillus plantarum on fungal control and egg hatching performance of rainbow trout (Oncorhynchus mykiss)