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بهبود خصوصیات فیزیولوژیک و بیوشیمیایی آرابیدوبسیس تالیانا با انتقال تراژن آرتمین | ||
علوم و تحقیقات بذر ایران | ||
مقاله 7، دوره 9، شماره 4، دی 1401، صفحه 75-91 اصل مقاله (1.4 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22124/jms.2023.6172 | ||
نویسندگان | ||
طیبه فلاحی پاشاکی؛ محمد مهدی سوهانی* ؛ رضا شیرزادیان خرم آباد | ||
گروه بیوتکنولوژی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت | ||
چکیده | ||
دمای بالا سبب آسیبهای جدی در گیاهان از جمله اختلال در تعادل درون سلولی، کندی یا رکود رشد و نمو و حتی مرگ میشود. هنگامی که گیاهان در معرض دمای بالا قرار میگیرند، پروتئینهای پاسخ گرمایی (HSP)در سلول فعال میشوند. از همین گروه، HSPهای کوچک (sHSP) قرار دارند که با افزایش دما فعال شده، و در نقش چاپرون مولکولی به پروتئینهایی که در معرض تنش گرمایی بودهاند متصل میشوند و مانع از تاشدن نادرست یا به عبارتی به تاخوردگی صحیح آنها کمک میکنند. پروتئینی به نام آرتمین در سخت پوست Artemia urmianaشناسایی شد که از مهمترین خصوصیات آن قدرت چپرونی بـالا و وجود دمین α-crystallin است که وجه مشخصهsHSPها است. بهمنظور مطالعات ژنومیکس کارکردی شامل فنوتیپ و شاخصهای تحمل گیاهان، تراژن آرتمین به گیاه مدل آرابیدوپسیس تالیانا منتقل و واکنش گیاهان موتانت در قالب یک طرح آزمایشی بهصورت فاکتوریل و طرح پایه کاملاً تصادفی در سه تکرار درآزمایشگاه بیوتکنولوژی مطالعه شد. تنش گرمایی در دمای 45 درجه سلسیوس اعمال و مطالعه مولفههای بیوشیمیایی، مولفههای جوانهزنی و فیزیولوژیک نشان داد که برهمکنش فاکتورهای تنش گرمایی و ژنوتیپ بر تمام صفات مربوط به جوانهزنی، وزنتر و وزن خشک گیاهچه، طول گیاهچه و محتوای پروتئین کل، آنزیم کاتالاز و محتوای مالوندیآلدئید در سطح یک درصد معنیدار بودند. با اعمال تنش گرمایی گیاهان حاوی تراژن آرتمین به طور معنیداری نسبت به گیاهان غیرتراریخته جوانهزنی و رشد گیاهچه بهتری را نشان دادند. تنش همچنین موجب افزایش معنیدار فعالیت آنزیم کاتالاز و محتوای پروتئین کل و کاهش محتوای مالوندیآلدئید در گیاهان تراریخته در مقایسه با گیاه غیرتراریخته شد. بر این اساس، حضور تراژن آرتمین در گیاهان احتمالا سبب افزایش مقاومت گیاهچه به تنش گرمایی و افزایش عملکرد میشود. | ||
کلیدواژهها | ||
آرتمین؛ تنش غیرزیستی؛ جوانهزنی؛ محتوای پروتئین کل؛ sHSP | ||
مراجع | ||
Aebi, U., Cohn, J., Buhle, L. and Gerace, L. 1986. The nuclear lamina is a meshwork of intermediate-type filaments. Nature , 323: 560-564. (Journal) Bajji, M., Kinet, J.M. and Lutts, S. 2002. Osmotic and ionic effects of NaCl on germination, early seedling growth, and ion content of Atriplex halimus (Chenopodiaceae). Canadian journal of botany, 80: 297-304. (Journal) Basra, S., Afzal, I., Rashid, R. and Hameed, A. 2005. Inducing salt tolerance in wheat by seed vigor enhancement techniques. International Journal of Agriculture and Biology, 2: 173-179. (Journal) Begcy, K., Sandhu, J. and Walia, H. 2018. Transient heat stress during early seed development primes germination and seedling establishment in rice. Frontiers in Plant Science, 9: 1768. (Journal) Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry, 72(1-2): 248-254. (Journal)
Bray, E.A. 2000. Response to abiotic stress. Biochemistry and molecular biology of plants, 1158-1203. (Journal) Changjun, L., Liang, B., Shengwei, T. and Enze, M. 2013. Effects of orifice orientation and gas-liquid flow pattern on initial bubble size. Chinese Journal of Chemical Engineering , 21: 1206-1215. (Journal) Clegg, J.S. 2007. Protein stability in Artemia embryos during prolonged anoxia. The Biological Bulletin, 212: 74-81. (Journal) Clegg, J.S., Willsie, J.K. and Jackson, S. A. 1999. Adaptive significance of a small heat shock/α-crystallin protein (p26) in encysted embryos of the brine shrimp, Artemia franciscana. American Zoologist, 39: 836-847. (Journal) Crowe, J.H., Hoekstra, F.A. and Crowe, L.M. 1992. Anhydrobiosis. Annual review of physiology, 54: 579-599. (Journal) Efeoğlu, B. 2009. Heat shock proteins and heat shock response in plants. Gazi University Journal of Science, 22: 67-75. (Journal) Fahad, S., Bajwa, A.A., Nazir, U., Anjum, S.A., Farooq, A., Zohaib, A. and Saud, S. 2017. Crop production under drought and heat stress: plant responses and management options. Frontiers in plant science, 1147. (Journal) Ferrer, M., Travierso, C., Cilloniz, C., Gabarrus, A., Ranzani, O.T., Polverino, E., Liapikou, A., Blasi, F. and Torres, A. 2018. Severe community-acquired pneumonia: Characteristics and prognostic factors in ventilated and non-ventilated patients. PloS one, 13: e0191721. (Journal) Finch-Savage, W.E. and Bassel, G.W. 2016. Seed vigour and crop establishment: extending performance beyond adaptation. Journal of experimental botany, 67: 567-591. (Journal) Formentin, E., Sudiro, C., Ronci, M.B., Locato, V., Barizza, E., Stevanato, P., Ijaz, B., Zottini, M., De Gara, L. and Lo Schiavo, F. 2018. H2O2 signature and innate antioxidative profile make the difference between sensitivity and tolerance to salt in rice cells. Frontiers in plant science, 9: 1549. (Journal) Granier, C., Massonnet, C., Turc, O., Muller, B., Chenu, K. and Tardieu, F. 2002. Individual leaf development in Arabidopsis thaliana: a stable thermal‐time‐based programme. Annals of botany, 89: 595-604. (Journal) Härndahl, U., Hall, R.B., Osteryoung, K.W., Vierling, E., Bornman, J. F. and Sundby, C. 1999. The chloroplast small heat shock protein undergoes oxidation-dependent conformational changes and may protect plants from oxidative stress. Cell stress and chaperones, 4: 129. (Journal) Hatfield, J.L., Antle, J., Garrett, K.A., Izaurralde, R.C., Mader, T., Marshall, E., Nearing, M., Philip Robertson, G. and Ziska, L. 2020. Indicators of climate change in agricultural systems. Climatic Change, 163: 1719-1732. (Journal) Heath, R.L. and Packer, L. 1968. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of biochemistry and biophysics, 125: 189-198. (Journal) Hofmann, N.R. 2009. The plasma membrane as first responder to heat stress. The Plant Cell, 21: 2544-2544. (Journal) Hussain, H.A., Men, S., Hussain, S., Chen, Y., Ali, S., Zhang, S., Zhang, K., Li, Y., Xu, Q. and Liao, C. 2019. Interactive effects of drought and heat stresses on morpho-physiological attributes, yield, nutrient uptake and oxidative status in maize hybrids. Scientific reports, 9: 1-12. (Journal) Iloh, A., Omatta, G., Ogbadu, G. and Onyenekwe, P. 2014. Effects of elevated temperature on seed germination and seedling growth on three cereal crops in Nigeria. Scientific research and essays, 9: 806-813. (Journal) IPCC, 2018. Summary for Policymakers. In: Global Warming of 1.5°C. (An IPCC Special Report on the impacts of global warming of 1.5°C above pre-industrial levels and related global greenhouse gas emission pathways, in the context of strengthening the global response to the threat of climate change, sustainable development, and efforts to eradicate poverty). (Report) Jiang, C., Xu, J., Zhang, H., Zhang, X., Shi, J., Li, M. and Ming, F. 2009. A cytosolic class I small heat shock protein, RcHSP17. 8, of Rosa chinensis confers resistance to a variety of stresses to Escherichia coli, yeast and Arabidopsis thaliana. Plant, Cell & Environment, 32: 1046-1059. (Journal)
Kadota, Y. and Shirasu, K. 2012. The HSP90 complex of plants. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1823(3): 689-697. (Journal) Kar, M. and Mishra, D. 1976. Catalase, peroxidase, and polyphenoloxidase activities during rice leaf senescence. Plant physiology, 57: 315-319. (Journal) Koo, H.J., Park, S.M., Kim, K.P., Suh, M.C., Lee, M.O., Lee, S.K., Xinli, X. and Hong, C. B. 2015. Small heat shock proteins can release light dependence of tobacco seed during germination. Plant Physiology, 167: 1030-1038. (Journal) Kumar, M., Padula, M. P., Davey, P., Pernice, M., Jiang, Z., Sablok, G. and Ralph, P. J. 2017. Proteome analysis reveals extensive light stress-response reprogramming in the seagrass Zostera muelleri (Alismatales, Zosteraceae) metabolism. Frontiers in plant science, 7: 2023. Kumar, M., Liu, Y., Katul, G.G. and Porporato, A.M. 2019. Detecting Climate-Stress Induced Forest Mortality Before the Canonical Symptoms Appear. In "AGU Fall Meeting Abstracts", Vol. 2019, pp. B13K-2608. (Journal) Lata, C., Yadav, A. and Prasad, M. 2011. Role of plant transcription factors in abiotic stress tolerance. Abiotic Stress Response in Plants, INTECH Open Access Publishers, 8(10): 269-296. (Journal) Li, J., Zhang, J., Jia, H., Li, Y., Xu, X., Wang, L. and Lu, M. 2016. The Populus trichocarpa PtHSP17. 8 involved in heat and salt stress tolerances. Plant cell reports, 35: 1587-1599. (Journal) Liberek, K., Lewandowska, A. and Ziętkiewicz, S. 2008. Chaperones in control of protein disaggregation. The EMBO journal, 27: 328-335. (Journal) Lindquist, S. and Craig, E.A. 1988. The heat-shock proteins. Annual review of genetics, 22: 631-677. (Journal) Lobell, D.B. and Asner, G.P. 2003. Climate and management contributions to recent trends in US agricultural yields. Science, 299: 1032-1032. (Journal) Maguire, J.D. 1962. Speed of germination-aid in selection and evaluation for seedling emergence and vigor. Crop Science, 2: 176-177. (Journal) Malik, M.K., Slovin, J.P., Hwang, C.H. and Zimmerman, J.L. 1999. Modified expression of a carrot small heat shock protein gene, Hsp17. 7, results in increased or decreased thermotolerance. The Plant Journal, 20: 89-99. (Journal) McCord, J.M. 2008. Superoxide dismutase, lipid peroxidation, and bell-shaped dose response curves. Dose-Response, 6: 8-012. (Journal) Nadia, A. and Hanaa, A. 2019. Extraction of high-quality genomic DNA from different plant orders applying a modified CTAB-based method. Aboul-Maaty and Oraby Bulletin of the National Research Centre, 43: 43-25. (Journal) Nakamoto, H. and Vigh, L. 2007. The small heat shock proteins and their clients. Cellular and Molecular Life Sciences, 64: 294-306. (Journal) Pastenes, C. and Horton, P. 1996. Effect of high temperature on photosynthesis in beans (II. CO2 assimilation and metabolite contents). Plant Physiology, 112: 1253-1260. (Journal) Pourmohammad, Z.K. 2021. The role of artemin in (Arabidopsis thaliana) response to abiotic stresses. Master Dissertation, University of Guilan, Faculty of Agricultural Sciences, Department of Agricultural Biotechnology Iran. (In Persian) (Thesis) Qaseem, M.F., Qureshi, R. and Shaheen, H. 2019. Effects of pre-anthesis drought, heat and their combination on the growth, yield and physiology of diverse wheat (Triticum aestivum L.) genotypes varying in sensitivity to heat and drought stress. Scientific reports, 9: 1-12. (Journal) Qi, F. and Zhang, F. 2020. Cell cycle regulation in the plant response to stress. Frontiers in plant science, 10: 1765. (Journal) Queitsch, C., Sangster, T.A. and Lindquist, S. 2002. Hsp90 as a capacitor of phenotypic variation. Nature, 417: 618-624. (Journal) Ramana, S., Biswas, A., Kundu, S., Saha, J. and Yadava, R. 2002. Effect of distillery effluent on seed germination in some vegetable crops. Bioresource technology, 82(3): 273-275. (Journal) Reinking, L. 2007. Imagej basics. Word Journal Of The International Linguistic Association, 1-22. (Journal) Rezayian, M., Niknam, V. and Ebrahimzadeh, H. 2018. Effects of drought stress on the seedling growth, development, and metabolic activity in different cultivars of canola. Soil Science and Plant Nutrition, 64: 360-369. (Journal)
Sable, A. and Agarwal, S.K. 2018. Plant heat shock protein families: essential machinery for development and defense. Journal of Biological Sciences and Medicine, 4: 51-64. (Journal) Saidi, Y., Finka, A. and Goloubinoff, P. 2011. Heat perception and signalling in plants: a tortuous path to thermotolerance. New phytologist, 190: 556-565. (Journal) Sarkar, N. K., Kotak, S., Agarwal, M., Kim, Y.-K. and Grover, A. 2020. Silencing of class I small heat shock proteins affects seed-related attributes and thermotolerance in rice seedlings. Planta, 251: 1-16. (Journal) Salisbury, F.B. and Ross, C.W. 1992. Plant Physiology. Wadsworth Publishing Co. Inc. California. (Book) Shahangian, S.S., Rasti, B., Sajedi, R.H., Khodarahmi, R., Taghdir, M. and Ranjbar, B. 2011. Artemin as an efficient molecular chaperone. The protein journal, 30: 549-557. (Journal) Sørensen, J.G., Kristensen, T.N. and Loeschcke, V. 2003. The evolutionary and ecological role of heat shock proteins. Ecology letters, 6: 1025-1037. (Journal) Takalloo, Z., Masroor, M.J., Mani-Varnosfaderani, A., Maroufi, B. and Sajedi, R.H. 2020. Probing heat and oxidation induced conformational changes of molecular chaperone artemin by excitation-emission fluorescence spectroscopy. Journal of Photochemistry and Photobiology B: Biology, 211: 112013. (Journal) Takalloo, Z., Sajedi, R.H., Hosseinkhani, S. and Asghari, S.M. 2016. Real-time monitoring of artemin in vivo chaperone activity using luciferase as an intracellular reporter. Archives of Biochemistry and Biophysics, 610: 33-40. (Journal) Takalloo, Z., Sajedi, R.H., Hosseinkhani, S. and Moazzenzade, T. 2017. Artemin protects cells and proteins against oxidative and salt stress. International journal of biological macromolecules, 95: 618-624. (Journal) Utami, D. and Aryanti, E. 2021. Impact of heat stress on germination and seedling growth of chili pepper (Capsicum annuum L.). In "IOP Conference Series: Earth and Environmental Science", 637:12032. (Journal) Vacca, R.A., De Pinto, M.C., Valenti, D., Passarella, S., Marra, E. and De Gara, L. 2004. Production of reactive oxygen species, alteration of cytosolic ascorbate peroxidase, and impairment of mitochondrial metabolism are early events in heat shock-induced programmed cell death in tobacco Bright-Yellow 2 cells. Plant physiology, 134: 1100-1112. (Journal) Wang, M., Zou, Z., Li, Q., Sun, K., Chen, X. and Li, X. 2017. The CsHSP17. 2 molecular chaperone is essential for thermotolerance in Camellia sinensis. Scientific reports, 7: 1-15. (Journal) Wang, W., Vinocur, B., Shoseyov, O. and Altman, A. 2004. Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response. Trends in plant science, 9: 244-252. (Journal) Waters, E.R., Lee, G.J. and Vierling, E. 1996. Evolution, structure and function of the small heat shock proteins in plants. Journal of Experimental Botany, 47: 325-338. (Journal) Willsie, J.K. and Clegg, J.S. 2001. Nuclear p26, a small heat shock/α-crystallin protein, and its relationship to stress resistance in Artemia franciscana embryos. Journal of Experimental Biology, 204: 2339-2350. (Journal) Yan, A., Wu, M., Yan, L., Hu, R., Ali, I. and Gan, Y. 2014. AtEXP2 is involved in seed germination and abiotic stress response in Arabidopsis. PloS one, 9: 85208. (Journal) Yang, R., Yu, G., Li, H., Li, X. and Mu, C. 2020. Overexpression of small heat shock protein LimHSP16. 45 in Arabidopsis hsp17. 6II mutant enhances tolerance to abiotic stresses. Russian journal of plant physiology, 67: 231-241. (Journal) Zeinoddini, M., Khajeh, K., Hosseinkhani, S., Saeedinia, A.R. and Robatjazi, S.M. 2013. Stabilisation of recombinant aequorin by polyols: activity, thermostability and limited proteolysis. Applied biochemistry and biotechnology, 170: 273-280. (Journal) Zhang, X., Henriques, R., Lin, S.S., Niu, Q.W. and Chua, N.H. 2006. Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method. Nature protocols, 1: 641-646. (Journal) Zhou, Y., Chen, H., Chu, P., Li, Y., Tan, B., Ding, Y., Tsang, E.W., Jiang, L., Wu, K. and Huang, S. 2012. NnHSP17. 5, a cytosolic class II small heat shock protein gene from Nelumbo nucifera, contributes to seed germination vigor and seedling thermotolerance in transgenic Arabidopsis. Plant cell reports, 31: 379-389. (Journal) | ||
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