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اجزای شیمیایی، سمیت تدخینی و تاثیر بیوشیمیایی و ضد تغذیهای اسانس Eucalyptus oleosa Muell. روی سوسک کشیش، Rhyzopertha dominica (F.) | ||
تحقیقات آفات گیاهی | ||
مقاله 4، دوره 12، شماره 2، شهریور 1401، صفحه 35-50 اصل مقاله (853.83 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22124/iprj.2022.5793 | ||
نویسنده | ||
عسگر عباداللهی* | ||
گروه تولیدات گیاهی، دانشکده کشاورزی و منابع طبیعی مغان، دانشگاه محقق اردبیلی، اردبیل، ایران. | ||
چکیده | ||
سوسک کشیش، Rhyzopertha dominica (F.)، باعث ایجاد خسارات کمی و کیفی گستردهای به دانههای غلات انباری میشود. استفاده از ترکیبات شیمیایی ساختگی در مدیریت چنین آفاتی آثار جانبی زیادی از قبیل آلودگی محیط زیست، تهدید سلامتی انسان و توسعه مقاومت آفات را در پی داشته است. توانمندی حشرهکشی نویدبخش اسانسهای استخراج شده از گونههای مختلف جنس Eucalyptus روی حشرات آفت در پژوهشهای اخیر نشان داده شده است. در این تحقیق، تاثیر کشندگی و زیرکشندگی اسانس E. oleosa Muell. روی حشرات کامل R. dominica بررسی شد. تجزیه شیمیایی اسانس نشان داد که ترکیبات ترپنی نظیر 1,8-cineole، α-pinene، trans-pinocarveol، (S)-α-terpineol و β-eudesmol واجد بالاترین مقدار بودند. حشرات کامل در برابر تدخین اسانس حساس بودند؛ بهطوریکه مقادیر LC50 (غلظت کشنده 50 درصد) محاسبه شده بعد از 24، 48 و 72 ساعت بهترتیب 923/42، 828/35 و 044/34 میکرولیتر بر لیتر هوا برآورد شد. با وجود افزایش فعالیت آنزیمهای آلفا- و بتا-استراز، محتوای پروتئین، گلیکوژن و لیپید کل و فعالیتهای آمیلولیتیک و پروتئولیتیک حشرات کامل تیمار شده با LC30 (غلظت کشنده 30 درصد) اسانس به صورت معنیداری در مقایسه با شاهد کاهش یافت. شاخص مصرف (CI)، نرخ مصرف نسبی (RCR) و نرخ رشد نسبی (RGR) هم در حشرات کامل تیمار شده بهصورت معنیداری کاهش یافت. شاخص بازدارندگی تغذیهای (FDI) 40/59 درصدی تحت تاثیر غلظت کشنده 30 درصد اسانس در حشرات تیمار شده محاسبه شد. با توجه به تاثیر کشندگی و زیرکشندگی مشاهده شده، اسانس E. oleosa میتواند برای انجام بررسیهای تکمیلی در راستای مدیریت R. dominica مورد توجه قرار گیرد. | ||
کلیدواژهها | ||
اسانس گیاهی؛ تاثیر کشندگی؛ تاثیر زیرکشندگی؛ ترکیبات ترپنی؛ خواص حشرهکشی | ||
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