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اثرات کشندگی و زیرکشندگی اسانس آزاد و نانوکپسوله پونه Mentha longifolia روی شبپره مدیترانهای آرد Anagasta kuehniella (Lep.: Pyralidae) | ||
تحقیقات آفات گیاهی | ||
مقاله 2، دوره 12، شماره 2، شهریور 1401، صفحه 1-19 اصل مقاله (997.76 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22124/iprj.2022.5791 | ||
نویسنده | ||
مریم ملکمحمدی* | ||
گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه بوعلیسینا، همدان، ایران | ||
چکیده | ||
در بررسی قبلی، نانوسیلیکای حاوی اسانس پونهMentha longifolia L. (Lamiaceae) به روش سل- ژل با هدف امکانسنجی کاربرد علیه جمعیتهای آفت ساخته شد. موفقیت روش بر اساس شاخص پراکندگی، راندمان ریزپوشانی، پتانسیل زتا و متوسط اندازه ذرات تایید شد. پژوهش حاضر با هدف بررسی سمیت فرمولاسیون آزاد و نانوکپسول اسانس پونه به دو روش تنفسی روی مراحل تخم، لاروهای سن پنجم و حشرات بالغ در بازههای زمانی 24، 48 و 72 ساعته و موضعی روی لاروهای سن پنجم شبپره مدیترانهای آرد Anagasta kuehniella Zeller (Lepidoptera: Pyralidae)صورت گرفت. ارزیابی اثرات زیرکشندگی در غلظت زیرکشنده (LC25) به روش تدخینی و از طریق برآورد درصد بازدارندگی از تخمگذاری، درصد بارآوری، درصد خروج حشرات بالغ و نرخ جفتگیری هدف دیگر این مطالعه بود. نتایج زیستسنجیها به روش تنفسی، همسویی سمیت تنفسی و تلفات جمعیتها با غلظت اسانس مصرفی و زمان در معرض قرارگیری را تایید نمود. ساخت نانو سیلیکای اسانس پونه، افزایش 62/1 برابری سمیت موضعی و کشندگی نانوذرات اسانس در مقایسه با شکل آزاد اسانس علیه مرحله پنجم لاروی شبپره مدیترانهای آرد را به دنبال داشت. یافتهها به وضوح اثرات منفی زیرکشندگی اسانس آزاد پونه و نانوذرات آن در مقایسه با شاهد را بر تعداد تخمهای گذاشته شده به ازای هر حشره ماده (بهترتیب 63 و 42 تخم به ازای هر حشره ماده)، درصد تفریخ تخمهای گذاشته شده (بهترتیب 1/19% و 6/17%)، درصد خروج حشرات بالغ (بهترتیب 4/16 و 3/11%) و نرخ جفتگیری (بهترتیب 48/6 و 6/3%) در پایان 72 ساعت تایید نمود. یافتههای حاصل از زیستسنجی، ضمن تایید سمیت تنفسی و موضعی اسانس آزاد و نانوکپسوله پونه، اثرات اختلالی اسانس بر پراسنجههای بیولوژیکی شبپره مدیترانهای آرد را نیز گزارش نمود. اثرات کشندگی و زیرکشندگی قویتر نانوذرات اسانس پونه در مقایسه با اسانس آزاد، استفاده از فرمولاسیون نانوی اسانسهای گیاهی به عنوان گزینه جایگزین یا مکمل حشرهکشهای مرسوم در مبارزه با آفات انباری را پیشنهاد مینماید. | ||
کلیدواژهها | ||
اثرات کشندگی و زیرکشندگی؛ اسانس؛ ریزپوشانی؛ شبپره مدیترانهای آرد | ||
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