تعداد نشریات | 31 |
تعداد شمارهها | 743 |
تعداد مقالات | 7,045 |
تعداد مشاهده مقاله | 10,199,093 |
تعداد دریافت فایل اصل مقاله | 6,839,695 |
Susceptibility analysis of Arcobacter isolated from fresh cheeses from municipal markets to fluoroquinolones in Guaranda City, Ecuador | ||
Caspian Journal of Environmental Sciences | ||
دوره 20، شماره 2، تیر 2022، صفحه 393-399 اصل مقاله (698.01 K) | ||
نوع مقاله: Research Paper | ||
شناسه دیجیتال (DOI): 10.22124/cjes.2022.5584 | ||
نویسندگان | ||
Favian Bayas-Morejón* ؛ Riveliño Ramón-Curay؛ Jagger Segura-Ochoa؛ Jenny Martínez | ||
Universidad Estatal de Bolívar, Facultad de Ciencias Agropecuarias Recursos Naturales y del Ambiente, Departamento de Investigación; CP: 020150, Guaranda-Ecuador | ||
چکیده | ||
some species of the Arcobacter genus are considered emerging foodborne enteropathogens. However, the presence of this bacterium in cheese is little known. On the other hand, quinolones are considered first-line drugs for the treatment of campylobacter infection in human patients, but currently little data is available on the levels of resistance to these antibiotics among Arcobacter species. Thus, the objective of this study was to analyze the susceptibility of Arcobacter spp. isolated from 100 fresh cheeses, obtained from the municipal markets to Fluoroquinolones in Guaranda City, Ecuador. By culture, 47 out of the 100 cheese samples were positive with a total of 66 isolates. By PCR, the number of positive samples was reduced to 21 with 26 isolates. With respect to antimicrobial activity, 2 isolates showed resistance to Levofloxacin (7.69%) and 6 to Ciprofloxacin (23.08%). Nineteen strains exhibited intermediate resistance to Levofloxacin and 10 to Ciprofloxacin. This study is the first report on the presence of pathogenic species of Arcobacter spp. in fresh cheeses in Ecuador, which could act as a vehicle for transmission to humans and pose a potential risk to public health. | ||
کلیدواژهها | ||
Arcobacter؛ Isolation؛ Resistance؛ Fluoroquinolones | ||
مراجع | ||
Abdelbaqi, K, Ménard, A, Prouzet Mauleon, V, Bringaud, F, Lehours, P & Mégraud, F 2007, Nucleotide sequence of the gyrA gene of Arcobacter species and characterization of human ciprofloxacin resistant clinical isolates. FEMS Immunology and Medical Microbiology, 49: 337-345.
Aldred, KJ, Kerns, RJ & Osherof, N 2014, Mechanism of Quinolone Action and Resistance. Biochemistry. 47: 53. 1565-1574.
Atabay, HI, Waino, HI & Madsen, M 2006, Detection and diversity of various Arcobacter species in 53 Danish poultry. International Journal of Food Microbiology, 109: 139-145.
Bayas, I 2016, Contributions to the epidemiology of arcobacter and Helicobacter spp.: Application of molecular methods to their detection and identification in food (online). S.l., Polytechnic University of Valencia. 43-44. bayas - aportaciones a la epidemiología de Arcobacter & Helicobacter spp. %3a aplicación de métodos pdf?sequence =1%0ahttps://riunet.upv .es/bitstream/handle/10251/75087/ bayas - aportaciones a la epidemi.
Bayas, I, Gonzalez, A & Ferrús, M 2016, Detection and identification of Arcobacter spp. and Helicobacter pylori in food by conventional and molecular methods. IV Congress of Science, Technology, Innovation and Entrepreneurship, Bolivar-Ecuador, November, 16-18.
Bayas-Morejón, F, Ramón, R, García-Pazmiño, M, Mite-Cárdenas, G 2020, Antibacterial and antioxidant effect of natural extracts from Baccharis latifolia (chilca), Caspian Journal of Environmental Sciences, 18: 489-493
Calvo, G, Fernándes, H & Laura, M 2013, Arcobacter: An emerging foodborne pathogen. Archivos Latinoamericanos de Nutricion, 63: 2.
CLSI, Peformance standards for antimicrobial susceptibility testing. 27th ed. CLSI Supplement m100. Wayne-Pennsylvania.
Collado, L & Figueras, M 2012, Higher water temperature and incubation under aerobic and microaerobic conditions increase the recovery and diversity of Arcobacter spp. from shellfish (online). S.l., Universidad Austral de Chile, 385-391.
European Committee on Antimicrobial Susceptibility Testing 2018, Data from the EUCAST MIC EUCAST break points, last access Thursday July 25, 2019 16. FAO. (2018). Dairy market review (online). S.l., s.e, 1-2.
FAO; PAHO 2017, Food and nutrition security overview (online). S.l., S.E., 1-174.
Figueras, MA, Pérez Cataluña, A, Salas Massó, N, Levican, A & Collado, L 2017, Arcobacter porcinus sp. nov., a novel Arcobacter species uncovered by Arcobacter thereius. New Microbes and New Infections, 15: 104-106.
García, A, Méndez, C, Elton Puente, E, Abrego, R, Castillo, M & Rodríguez, F 2015, Determination of the microbiological quality of dairy products produced in a higher education institution. Faculty of Chemical Sciences, Autonomous University of Querétaro. Queretaro Mexico, 25-28.
Giacometti, F, Lucchi, A, Manfreda, G, Florio, D, Zanoni, RG & Serraino, A 2013, Occurrence and genetic diversity of Arcobacter butzleri in an artisanal dairy plant in Italy. Applied and Environmental Microbiology, 79: 6665-6669.
González, A & Ferrús, MA, 2011, Study of Arcobacter spp. contamination in fresh lettuces detected by different cultural and molecular methods. International Journal of Food Microbiology, 145: 311-314.
González, A, Bayas Morejón, IF & Ferrús, MA 2017, Isolation, molecular identification and quinolone-susceptibility testing of Arcobacter spp. isolated from fresh vegetables in Spain, Food Microbiology, DOI: 10.1016/j.fm.2017.02.011
Guamán, J 2017, Implementation of a bacterial DNA extraction method 57 for the detection of Helicobacter pylori in human stool samples. (Postgraduate Dissertation). Guayaquil University, Guayaquil, Ecuador, 35-36.
Houf, K, Devriese, A, De Zutter, L, Van Hoof, J & Vandamme, Pi 2001, Development of a new protocol for the isolation and quantification of Arcobacter species from poultry products. International Journal of Food Microbiology, 71: 189-196.
Luz, P & Roy, V 2017, Technical improvement plan for food handling and preservation in the San Jacinto municipal market (Juan Montalvo cooperative) (online). S.l., Guayaquil University, 5-6 pm, Available at: http://repositorio.ug.edu.ec/bitstreami/redug/20813/1/tesis gs. 217 - improvement plan tecn handling conserve.food.pdf.
Mansilla, I 2006, Comparative study of the genotyping methods by Arcobacter butzleri, Arcobacter cryaerophilus and Arcobacter skirrowii isolates from divers reservoirs (online). S.l., Australian University of Chile, 3-4, [In Spanish].
Merga, JY, Leatherbarrow, AJ, Winstanley, C, Bennett, M, Hart, CA, Miller, WG, Williams, NJ 2011, Comparison of Arcobacter isolation methods, and diversity of Arcobacter spp. in Cheshire, United Kingdom. Applied and Environmental Microbiology, 77. 16461650.
Mottola, A, Bonerba, E, Figueras, MJ, Pérez Cataluña, A, Marchetti, P, Serraino, A, Bozzo, G, Terio, V, Tantillo, G & Di Pinto, A 2016, Occurrence of potentially pathogenic Arcobacters in shellfish. Food Microbiology, 57: 23-27.
Rivera, IG 2015, Determination of the presence of putative Arcobacter virulence genes in different growth media, temperatures and atmospheric conditions. (online), S.l., Pan American Agricultural School, Zamorano Honduras, 9-10.
Rojas, Z 2011, Evaluation of the specificity of two molecular methods for the identification of species belonging to the genus Arcobacter (online). S.l., Southern University of Chile, 3-4.
Sauca, W 2018, Isolation and detection of Arcobacter in raw milk by means of the multiplex PCR technique. (Project of Degree of Pharmaceutical Biochemist). UTPL, Loja.
Scarano, CI et al. 2014, Arcobacter butzleri in sheep ricotta cheese at retail and related sources of contamination in an industrial dairy plant. American Society of Microbiology, DOI: 10.1128/AEM.02491-14
Scullion, R, Harrington, CS & Madden, RH 2006, Prevalence of Arcobacter spp. in raw 38 milk and retail raw meats in Northern Ireland. Journal of Food Protection, i69: 1986– 1990.
M & Çelebi, Ö 2013, Antimicrobial susceptibilities of various Arcobacter species. Turkish Journal of Medical Sciences, 43: 548-552. DOI: 10.3906/sag-1207-115
Van den Abeele, AM, Vogelaers, D, Vanleare, E & Houf, K 2016, Antimicrobial susceptibility testing of Arcobacter butzleri and Arcobacter cryaerophilus strains isolated from Belgian patients. Journal of Antimicrobial Chemotherapy, 71: 1241-1244.
Vandenberg, O, Houf, K, Douat, N, Vlaes, L, Retore, P, Butzler, JP & Dediste, A 2006, Antimicrobial susceptibility of clinical isolates of non-jejuni/coli Campylobacters and Arcobacters from Belgium. Journal of Antimicrobial Chemotherapy, 57: 908-913.
Yesilmen, S, Vural, A, Erkan, ME & Yildirim, IH 2014, Prevalence and antimicrobial susceptibility of Arcobacter species in cow milk, water buffalo milk and fresh village cheese. International Journal of Food Microbiology, 188, 11-14. | ||
آمار تعداد مشاهده مقاله: 523 تعداد دریافت فایل اصل مقاله: 408 |