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بررسی تاثیر اسید سالسیلیک بر جوانهزنی و فعالیت برخی آنزیمهای آنتیاکسیدانی سیاهدانه (Nigella sativa L.) در شرایط تنش شوری | ||
علوم و تحقیقات بذر ایران | ||
مقاله 10، دوره 7، شماره 2، تیر 1399، صفحه 241-252 اصل مقاله (885.1 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22124/jms.2020.4580 | ||
نویسندگان | ||
بتول زارعی* 1؛ آرش فاضلی2؛ زهرا تقیپور3 | ||
1دکتری مهندسی ژنتیک، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه ایلام، ایلام، ایران | ||
2استادیار، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه ایلام، ایلام، ایران | ||
3دانشجوی دکتری مهندسی ژنتیک، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه ایلام، ایلام، ایران | ||
چکیده | ||
بهمنظور بررسی تاثیر پیشتیمار اسید سالسیلیک بر ویژگیهای جوانهزنی در شرایط تنش شوری، آزمایشی بهصورت فاکتوریل در قالب طرح پایه کاملاً تصادفی با سه تکرار انجام گرفت. فاکتورهای آزمایش شامل اسید سالسیلیک در سه سطح (صفر، 75/0و 5/1 میلیمولار) و تنش شوری در پنج سطح (صفر، 100، 200، 300 و 400 میلیمولار) بودند. اثرات ساده اسید سالسیلیک و تنش شوری بر صفات شاخص بنیه بذر، وزن تر و وزن خشک گیاهچه معنیدار بودند. اثرات متقابل اسید سالسیلیک و شوری بر سایر صفات در سطح احتمال یک درصد معنیدار بود. نتایج نشان داد که شوری بهطور معنیداری جوانهزنی و رشد گیاهچه را کاهش، اما اسید سالسیلیک شاخصهای جوانهزنی را بهبود بخشید و در تیمارهای تنشدیده سبب افزایش جوانهزنی شد. با افزایش سطح تنش شوری سرعت و درصد جوانهزنی کاهش یافت. بیشترین سرعت جوانهزنی در تیمار 5/1 میلیمولار اسید سالسیلیک در شرایط بدون شوری حاصل شد. پیشتیمار موجب افزایش معنیدار میزان فعالیت آنزیم کاتالاز نسبت به بدون پیشتیمار و کاهش میزان مالوندیآلدئید در تیمارهای دارای اسید سالسیلیک گردید. پیشتیمار با غلظت 5/1 میلیمولار ضمن اینکه تاثیر بیشتری بر جوانهزنی داشت، سبب افزایش مقاومت گیاهچههای سیاهدانه تحت تنش شوری نیز شد. | ||
کلیدواژهها | ||
اسید سالسیلیک؛ تنش شوری؛ جوانهزنی؛ کاتالاز؛ مالوندیآلدئید | ||
مراجع | ||
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