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تأثیر پیری بذر و هیدروپرایمینگ بر شاخصهای جوانهزنی و فعالیت برخی از آنزیم-های آنتیاکسیدانت هیبرید ذرت (Zea mays L.) | ||
علوم و تحقیقات بذر ایران | ||
مقاله 3، دوره 7، شماره 2، تیر 1399، صفحه 147-159 اصل مقاله (964.56 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22124/jms.2020.4543 | ||
نویسنده | ||
مجید عبدلی* | ||
دکتری فیزیولوژی گیاهان زراعی، گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه مراغه، مراغه، ایران | ||
چکیده | ||
بهمنظور بررسی اثر پیری تسریعشده و هیدروپرایمینگ بذر بر شاخصهای جوانهزنی و خصوصیات رشدی گیاهچه ذرت (سینگل کراس 701)، آزمایشی طی سال 1394 در آزمایشگاه پژوهشی دانشگاه مراغه بهصورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار اجرا شد. فاکتورهای آزمایش شامل سه سطح پیری تسریعشده بذر (صفر (شاهد)، 2 و 4 روز) و سه سطح پیشتیمار بذر (بدون پرایمینگ و هیدروپرایمینگ بهمدت 12 و 24 ساعت) بودند. نتایج تجزیه واریانس نشان داد که اثر ساده پیری بذر و هیدروپرایمینگ بر تمام صفات مورد مطالعه در سطح احتمال یک درصد معنیدار بود ولی فقط اثر متقابل پیری بذر و هیدروپرایمینگ بر فعالیت آنزیم آسکوربات پراکسیداز، میزان نشت یونی، درصد و سرعت جوانهزنی معنیدار بود. در این بین، اثرات ساده و متقابل پیری بذر و هیدروپرایمینگ بر نسبت طولی ساقهچه به ریشهچه غیرمعنیدار بود. نتایج حاصل از مقایسه میانگین نشان داد که در اثر پیری بذر، کلیه صفات مورد مطالعه کاهش یافتند بهطوریکه میزان پروتئینهای محلول، فعالیت آنزیم سوپراکسید دیسموتاز، طول ساقهچه، طول ریشهچه و شاخص بنیه بذر در اثر پیری 2 روزه بهترتیب بهمیزان 7/13، 9/10، 8/14، 8/14 و 2/33 درصد و در اثر پیری 4 روزه بهترتیب بهمیزان 8/36، 4/33، 4/32، 0/35 و 9/67 درصد نسبت به بذر شاهد (بدون پیری) کاهش یافتند. نتایج نشانگر این مطلب بود که با افزایش مدت زمان هیدوپرایمینگ بذور از 12 به 24 ساعت بر میزان پروتئینهای محلول، فعالیت آنزیم سوپراکسید دیسموتاز، طول ساقهچه، طول ریشهچه و شاخص بنیه بذر افزوده شد. بر اساس نتایج حاصل از اثرات متقابل مشخص گردید که در بذور شاهد اعمال هیدروپرایمینگ بهمدت 24 ساعت بیشترین و در بذور پیرشده (بهمدت 4 روز) عدم هیدروپرایمینگ کمترین فعالیت آنزیم آسکوربات پراکسیداز و درصد و سرعت جوانهزنی را داشتند. با توجه به نتایج حاصل از این تحقیق میتوان بیان کرد که هیدروپرایمینگ (مخصوصاً بهمدت 24 ساعت) میتواند برخی از اثرات مخرب و جزئی که طی پیری بذر رخ میدهند را کاهش دهد و از این تکنیک میتوان برای کاهش خسارات ناشی از پیری بذور ذرت بهره گرفت. | ||
کلیدواژهها | ||
بنیه بذر؛ پیشتیمار بذر؛ پیری تسریعشده؛ ذرت؛ طول گیاهچه | ||
مراجع | ||
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