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تأثیر اسید سالیسیلیک بر شاخصهای جوانهزنی، بنیه بذر و فعالیت برخی آنزیمهای آنتیاکسیدان آفتابگردان (Helianthus annuus L.) رقم آرماویرسکی تحت تنش خشکی | ||
علوم و تحقیقات بذر ایران | ||
دوره 7، شماره 1، خرداد 1399، صفحه 79-91 اصل مقاله (1.32 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22124/jms.2020.4342 | ||
نویسنده | ||
احمد افکاری* | ||
استادیار فیزیولوژی گیاهی، واحد کلیبر، دانشگاه آزاد اسلامی، کلیبر، ایران | ||
چکیده | ||
از آنجاییکه تولید گیاهان روغنی میتواند تحت تأثیر عوامل محیطی مانند محدودیت آب قرار گیرد، لذا تیمار با اسید سالیسیلیک بهعنوان یک تنظیمکننده رشد، قادر است مقاومت به خشکی را در گیاهان افزایش دهد. در این راستا، بهمنظور بررسی تأثیر اسید سالیسیلیک بر برخی خصوصیات بیوشیمیایی و جوانهزنی بذر گیاه آفتابگردان در شرایط تنش خشکی، آزمایشی بهصورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار در آزمایشگاه فیزیولوژی دانشگاه آزاد اسلامی واحد کلیبر در سال زراعی 1396 اجرا گردید. تیمارهای آزمایش شامل غلظتهای مختلف اسید سالیسیلیک در چهار سطح (صفر، 5/0، 1 و 5/1 میلیمولار) و سطوح مختلف تنش خشکی ایجادشده با پلیاتیلن گلایکول6000 در پنج سطح با پتانسیلهای صفر (آب مقطر)، 5-، 10-، 15- و 20- مگاپاسکال) بودند. نتایج نشان داد که اثر تنش خشکی و غلظتهای مختلف اسید سالیسیلیک بر مؤلفههای جوانهزنی و آنزیمهای آنتیاکسیدانت در سطح احتمال یک درصد معنیدار بود. برهمکنش تنش و اسید سالیسیلیک بر میزان فعالیت آنزیمهای سوپراکسید دیسموتاز، آسکوربات پراکسیداز، بنیه بذر، طول ریشهچه و ساقهچه معنیدار بود. نتایج مقایسه میانگین دادهها نشان داد که با افزایش تنش خشکی درصد و سرعت جوانهزنی کاهش و میزان فعالیت آنزیمهای آنتیاکسیدانت افزایش یافت. در پژوهش انجامشده کاربرد پیشتیمار اسید سالیسیلیک با غلظتهای 1 و5/0 میلیمولار باعث افزایش معنیدار فعالیت آنزیمهای مالون دیآلدئید، سوپراکسید دیسموتاز، گلوتاتیون پراکسیداز، کاتالاز، آسکوربات پراکسیداز و مؤلفههای جوانهزنی در مقایسه با شاهد گردید. نتایج کلی این آزمایش نشان داد که محلولپاشی اسید سالیسیلیک بهویژه در غلظت 1 میلیمولار با کاهش تنش اکسیداتیو ناشی از خشکی سبب بهبود شاخصهای جوانهرنی و افزایش در فعالیت آنزیمهای آنتیاکسیدانت در بذر آفتابگردان شد. | ||
کلیدواژهها | ||
آسکوربات پراکسیداز؛ آفتابگردان؛ اسید سالیسیلیک؛ بیومارکر؛ جوانهزنی؛ خشکی | ||
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