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زیستنشانگرهای آنزیمی پروانه برگخوارتوت، Glyphodes pyloalis Walker، در تماس با برخی نانومواد مورد کاربرد در فرمولاسیونهای نوین آفتکشی | ||
تحقیقات آفات گیاهی | ||
دوره 10، شماره 1، خرداد 1399، صفحه 69-85 اصل مقاله (1.16 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22124/iprj.2020.4107 | ||
نویسندگان | ||
نرگس معماریزاده* 1؛ محبوبه شریفی2 | ||
1بخش تحقیقات آفتکشها، موسسه تحقیقات گیاهپزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران | ||
2بخش تحقیقات گیاهپزشکی، مرکز تحقیقات کشاورزی و منابع طبیعی گلستان، سازمان تحقیقات، آموزش و ترویج کشاورزی،گرگان، ایران | ||
چکیده | ||
فعالیت استرازهای عمومی (ESTs)، کاتالاز (CAT) و گلوتاتیوناس-ترانسفراز (GST) پروانه برگ خوار توت (Glyphodes pyloalis Walker) برای بررسی واکنش داخل بدن (in vivo) حشره به نانولوله های کربنی (CNT) خالص و نانوذرات حاصل از اتصال نانولوله های کربنی به دیاکسید تیتانیوم (CNTs/TiO2-NPs) مورد ارزیابی قرار گرفت. این بررسی های بیوشیمیایی طی سه زمان مواجهه (24، 48 و 72 ساعت) با غلظت های مختلف این نانوذرات (100، 200، 300، 400 و 500 میلی گرم بر لیتر) انجام شد. نتایج نشان داد با به کارگیری آلفا-نفتیلاستات (α-NA) به عنوان سوبسترا، افزایش غلظتهای هر دو تیمار (یعنی CNT و CNTs/TiO2-NPs) باعث مختل شدن برگشت ناپذیر فعالیت استرازهای عمومی لاروهای سن پنجم این آفت می شود. این در حالی است که ارزیابی فعالیت آنزیم با به کارگیری بتا-نفتیلاستات (β-NA) به عنوان سوبسترا نشان دهنده تاثیر مهارکنندگی شدیدتر این نانوذرات بر فعالیت آنزیم استراز بود. نتایج همچنین نشان داد که بازدارندگی فعالیت آنزیمGST ناشی از CNT و CNTs/TiO2-NPs وابسته به غلظت تیمارها است. ارزیابی آنزیم CAT نشان داد که افزایش غلظت CNT در طول مدت زمان تماس، باعث افزایش فعالیت این آنزیم در G. pyloalis می شود. به رویه مشابه با CNT، افزایش زمان مواجهه و غلظت نانوذرات CNTs/TiO2-NPs باعث افزایش فعالیت آنزیم کاتالاز شد. بنابراین می توان نتیجه گیری کرد که آنزیم های مورد آزمون، شاخص های بیوشیمیایی مناسبی به منظور پیشبینی و مدیریت اثرات مخرب CNT خالص و CNTs/TiO2-NPs روی حشرات هستند. | ||
کلیدواژهها | ||
ارزیابی ریسک؛ نانوذرات؛ CNTs/TiO2-NPs؛ زیستنشانگرهای بیوشیمیایی | ||
مراجع | ||
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