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بررسی تاثیر سطوح مختلف باکتری Lactobacillus casei بر وضعیت دفاع آنتیاکسیدانی و برخی فراسنجههای بیوشیمیایی سرم خون ماهی قزلآلای رنگینکمان پس از مصرف سرب در جیره غذایی | ||
فیزیولوژی و بیوتکنولوژی آبزیان | ||
دوره 8، شماره 1، خرداد 1399، صفحه 37-68 اصل مقاله (1006.35 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22124/japb.2020.12716.1320 | ||
نویسندگان | ||
تکاور محمدیان* 1؛ رضا قانعی مطلق2؛ حسین خاج3؛ صادق رباط کریمی4؛ علی ضیاغم4؛ امین حیدری4 | ||
1دانشیار گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران | ||
2دکتری بهداشت آبزیان، گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران | ||
3استادیار موسسه تحقیقات واکسن و سرمسازی رازی، سازمان تحقیقات آموزش و ترویج کشاورزی، کرج، ایران | ||
4دانشجوی دکتری عمومی دامپزشکی، گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران | ||
چکیده | ||
هدف از مطالعه حاضر بررسی اثرات مصرف باکتری Lactobacillus casei روی برخی از فراسنجههای بیوشیمیایی و آنتیاکسیدانی سرم خون ماهی قزلآلای رنگینکمان پس از مواجهه با فلز سرب در جیره است. برای این منظور، 375 قطعه ماهی (6/4±15 گرم) به طور تصادفی به پنج گروه تقسیم شدند (با سه تکرار). گروه یک، دو و سه به ترتیب با جیرههای حاوی 106×5، 107×5 و CFU/g 108×5 پروبیوتیک تغذیه شدند. گروه چهارم (شاهد منفی) با جیره پایه تغذیه شد. گروه پنجم (شاهد مثبت) ابتدا به مدت 45 روز با جیره پایه و سپس به مدت 21 روز همراه با گروههای پروبیوتیکی با غذای حاوی نیترات سرب تغذیه شد. خونگیری در روزهای صفر، 45، 52، 59 و 66 صورت گرفت. طبق نتایج مقادیر LDH، کلسترول و تریگلیسرید در اغلب تیمارهای پروبیوتیکی پس از 45 روز نسبت به گروه شاهد منفی کاهش معنیداری داشت (05/0p <). پس از مواجهه با سرب مقادیر ALP و LDH سرم در گروه دو نسبت به پنج از کاهش معنیداری برخوردار بود (05/0p <). در گروه دو سطح آنزیم گلوتاتیون در روز 52 افزایش معنیداری نسبت به گروه پنج داشته است (05/0p <). بر اساس نتایج، استفاده از پروبیوتیک مذکور در ماهی قزلآلای رنگینکمان میتواند با بهبود شاخصهای بیوشیمیایی و آنتیاکسیدانی ماهی در پیشگیری از مسمومیت با سرب موثر باشد. همچنین به نظر میرسد که سطح CFU/g107×5 باکتری تاثیر بهتری در بهبود فراسنجههای مورد مطالعه داشت. | ||
کلیدواژهها | ||
پروبیوتیک؛ سرب؛ سرم خون؛ قزلآلا؛ لاکتوباسیلوس کازئی | ||
مراجع | ||
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