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مکانیابی و بررسی برهمکنش بین جایگاه های ژنومی کنترل کننده صفات مهم زراعی در برنج (Oryza sativa L.) | ||
تحقیقات غلات | ||
مقاله 2، دوره 8، شماره 4، اسفند 1397، صفحه 423-441 اصل مقاله (1.14 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22124/c.2019.12616.1468 | ||
نویسندگان | ||
حسین رحیم سروش1؛ فرهاد نظریان فیروزآبادی* 2؛ مریم حسینی چالشتری3؛ احمد اسماعیلی4؛ علی اکبر عبادی3 | ||
1دانشجوی دکتری،گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد،ایران | ||
2استاد،گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد،ایران | ||
3استادیار پژوهش، موسسه تحقیقات برنج کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، رشت،ایران | ||
4دانشیار،گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد، ایران | ||
چکیده | ||
به منظور شناسایی QTLهای اصلی و اپیستاتیک و برهمکنش آنها با محیط برای برخی صفات مهم زراعی برنج، تعداد 242 لاین خالص نوترکیب از دو جمعیت نسل F6حاصل از تلاقی IR67017-180-2-1-2 / علیکاظمی (جمعیتIRA) و صالح / علیکاظمی (جمعیتSA) در دو منطقه مورد ارزیابی قرار گرفتند. نقشه پیوستگی دو جمعیت با استفاده از 87 نشانگر ریزماهواره (SSR)، 1356 سانتیمورگان از ژنوم برنج را با فاصله متوسط 58/15سانتیمورگان بین نشانگرها پوشش داد. در مجموع بیست QTL اصلی و 33 QTL اپیستاتیک با آثار مثبت و منفی شناسایی شدند. تعداد پنج QTLاصلی و پنج QTL اپیستاتیک دارای برهمکنش معنیدار با محیط بودند و هشت QTLاصل یوده QTLاپیستاتیک کنترل کننده صفات زراعی مطلوب شناسایی شدند که هیچگونه برهمکنش معنیداری با محیط نداشتند. اینQTLها شامل: دوQTL اصلی و دو QTL اپیستاتیک برای کاهش ارتفاع بوته، سه QTLاصلی و سه QTL اپیستاتیک برای کاهش تاریخ خوشه دهی، یک QTLاصلی و یک QTL اپیستاتیک برای افزایش طول خوشه و دو QTL اصلی و چهارQTL اپیستاتیک برای افزایش عملکرد دانه بودند که بین 12/11 درصد (qHD6 ) تا 5/24 درصد (qGY1) از تنوع فنوتیپی صفات مربوطه را توجیهکردند. بنابراین، از این QTLهای مطلوب میتوان در برنامه هرمی کردن ژنها برای بهبود صفات مورد مطالعه استفاده کرد. همچنین، شش نشانگر پیوسته (RM421، RM178، RM3441، RM5101، RM7551 و RM5302) با فاصله کمتر از پنج سانتی مورگان با این QTLها شناسایی شدند که میتوان از آنها در برنامههای انتخاب بهکمک نشانگر به منظور به نژادی لاینهای برنج برای صفات مطلوب در نسلهای در حال تفکیک استفاده کرد. | ||
کلیدواژهها | ||
اپیستازی؛ عملکرد دانه؛ لاین های خویش آمیخته نوترکیب؛ نقشه پیوستگی | ||
مراجع | ||
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