پیوندهای مفید
آمار
| تعداد نشریات | 31 |
| تعداد شماره‌ها | 778 |
| تعداد مقالات | 7,449 |
| تعداد مشاهده مقاله | 19,591,435 |
| تعداد دریافت فایل اصل مقاله | 7,520,523 |
Experience in obtaining and cryopreserving semen of Mid-Caucasian Tur, Capra Caucasica Güldenstaedt et Pallas, 1783 | ||
| Caspian Journal of Environmental Sciences | ||
| دوره 23، شماره 1، فروردین 2025، صفحه 53-61 اصل مقاله (749.77 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22124/cjes.2025.8559 | ||
| نویسندگان | ||
| Marina I. Selionova* 1؛ Ali-Magomet M. Aybazov2؛ Evgeniya S. Latynina1 | ||
| 1Russian State Agrarian University, Moscow Timiryazev Agricultural Academy, Timiryazevskaya Street, 41, 127434 Moscow, Russia | ||
| 2North Caucasus Federal Scientific Agrarian Center, Stavropol Territory, Mikhailovsk, Russia | ||
| چکیده | ||
| The study was primarily aimed at determining the possibility of preserving the quality parameters and functional value of the semen of Caucasian tur, Capra caucasica after cryopreservation. For this purpose, the following objectives were set: to find out the possibility of obtaining the semen of Caucasian tur by electroejaculation and to study the parameters of its fresh undiluted semen; to study the cryoprotective properties of the experimental synthetic medium for dilution of its semen; to study the quality parameters and functional value of the frozen semen; and to evaluate the fertilizing capacity of the frozen and thawed semen. In the field study, semen was obtained during the breeding season (late November) from three mature turs by electroejaculation. A total of 19 ejaculates were obtained and evaluated. At each retrieval, ejaculates were pooled to exclude the influence of individual male characteristics and separated into two equal aliquots. One aliquot was then diluted with TRIS-based synthetic medium (control medium) and the other was diluted with the same medium supplemented with 10% platelet-rich plasma (PRP) from the same animal from which the biomaterial had been collected. Subsequently, all samples were cryopreserved in liquid nitrogen. The volume, total motility, velocity of sperm, acrosome, and plasma membrane integrity of sperm at different stages of cryopreservation (after dilution, equilibration, and freezing and thawing) were investigated. The results have shown that the semen obtained by electroejaculation had an average volume of 0.8 mL (range 0.3 to 1.4 mL), satisfactory quality parameters (sperm velocity 76% (7.6 points on a 10-point scale), average concentration 2.85 × 108 sperms mL-1). Semen diluted with the experimental medium supplemented with 10% PRP had significantly higher total motility (p < 0.05) compared to the control medium after equilibration and freezing, and thawing. In the experimental diluent after freezing and thawing, the percentage of sperm with high motility has significantly increased (p < 0.05) compared to the control medium. The addition of 10% PRP resulted in improved progressive motility and viability of sperm after cryopreservation (p < 0.05), as well as increased acrosome preservation and plasma membrane integrity compared to the control medium. The fertilizing ability of cryopreserved semen frozen in the experimental medium was 19.1% higher compared to the control medium. | ||
| کلیدواژه‌ها | ||
| Caucasian tur؛ Semen quality؛ PRP؛ Dilution؛ Cryopreservation؛ Field conditions | ||
| مراجع | ||
|
Aybazov, MM & Mamontova, TV 2014a, Some biological and morpho-metric indices of the West Caucasian tur. Sheep, Goats & Wool Business, 1: 21-23, [In Russian].
Aybazov, MM & Mamontova, TV 2021, Influence of cryoprotectors and technological processing on some quality parameters of frozen ram sperm. Zootechnics, 6: 33-37, (In Russian). https://doi.org/10.25708/ZT.2021.67.76.009.
Aybazov, MM & Selionova, MI 2024, Development of medium for dilution of semen of small ruminants. Agricultural Journal, 3:51-62, (In Russian), https://doi.org/10.48612/FARC/2687-1254/005.3.17. 2024.
Aybazov, MM, Trukhachev, VI, Selionova, MI & Malorodov, BB 2022, Seasonal changes in testis size, testosterone levels and semen production quality in meat rams. Reproduction in Domestic Animals, 57: 1125-1135, https://doi.org/10.1111/rda.14183.
Bader, R, Ibrahim, JN, Moussa, M, Mourad, A, Azoury, J, Alaaeddine, N 2020, In vitro effect of autologous platelet-rich plasma on H2O2-induced oxidative stress in human semenatozoa. Andrology 8: 191-200, https://doi.org/10.1111/andr12648.
Bagirov, VA, Aybazov, MM & Mamontova, TV 2014, Morphometric and biological features of the West Caucasian tur. Agricultural Journal, 7, [In Russian].
Bobyr, KG & Semyonov, UA 2008, West Caucasian tur in Teberdinsky reserve (ecology, physiology, diseases). Stavropol, 160 p, [In Russian].
Cassinello, J, Abaigar, T, Gomendio, M & Roldan, ERS 1998, Characteristics of the semen of three endangered species of gazelles (Gazella dama Mhorr, G. dorcas neglecta and G. cuvieri). Journal of Reproduction and Fertility, 113: 35-45.
Castellini, C, Mattioli, S, Dal Bosco, A, Collodel, G, Pistilli, A, Stabile, AM, Macchioni, L, Mancuso, F, Luca, G & Rende, M 2019, In vitro effect of nerve growth factor on the main traits of rabbit semen. Reproductive Biology and Endocrinology, 17: 1-11, https://doi.org/10.1186/s12958-019-0533-4.
Ferraguti, G, Fanfarillo, F, Tarani, L, Blaconá, G, Tarani, F, Barbato, C, Minni, A, Ralli, M, Francati, S, Greco, A, Petrella, C & Fiore, M 2022, NGF and the male reproductive system: potential clinical applications in infertility. International Journal of Molecular Sciences, 23: 13127, https://doi.org/10.3390/ijms232113127.
Greyling, JPC & Grobbelaar, JAN 1983, Seasonal variation in semen quality of Boer and Angora goat rams using different collection techniques. South African Journal of Animal Science, 13: 250-252.
Ivanina, LV & Tsvirko, NI 2018, To the question of protection of the Caucasian tur (Capra caucasica). Science and education: New time. Scientific and Methodological Journal, 4(11): 5-6, [In Russian].
Kargar, R, Forouzanfar, M, Ghalamkari, G & Esfahani, MHN 2017, Dietary flax seed oil and/or vitamin E improve semen parameters of cloned goats following freezing-thawing. Cryobiology, 74: 110-114. https://doi.org/10.1016/j.cryobiol.2016.11.007.
Kazanskaya, EY, Kuznetsova, MV & Danilkin, AA 2007, Phylogenetic reconstructions in the genus Capra (Bovidae, Artiodactyla) based on the mitochondrial DNA analysis. Russian Journal of Genetics, 43: 181-189.
Morozov YL 2009, Biology and economic use of turs. Dissertation, Candidate of Biological Sciences: Instituion of Defence: Russian State Agrarian Extramural University, Kirov, 145 p, [In Russian].
Mustofa, I, Susilowati, S, Wurlina, W, Hernawati, T & Oktanella, Y 2021, Green tea extract increases the quality and reduces DNA mutation of post-thawed Kacang buck semen. Heliyon, 7: e06372. https://doi.org/10.1016/j.heliyon.e06372.
Nasibov, SN, Voevodin, VA, Bagirov, VA, Iolchiev, BS, Klenovitsky, PM, Amirshoev, FS & Lesin, SA 2010а, Methods of obtaining and cryopreservation of seed for preservation of animal genetic resources. Bulletin of Kazan State Agrarian University, 5: 139-141, [In Russian].
Nasibov, SN 2010b, Genetic and biological aspects of hybridization of agricultural and wild animal species. Abstract of the Dissertation for the Degree of Doctor of Biological Sciences, Dubrovitsy, 23 p, [In Russian].
Pallas PS 1779, Capra caucasica e shedis cel A.J. Güldenstaedt. Acta Akademiae Scintiarum Imperialis Petropolitaneae, III.
Peris-Frau P, Soler AJ, Iniesta-Cuerda M, Martín-Maestro A, Sánchez-Ajofrín I, Medina-Chávez DA, Fernández-Santos MR, García-Álvarez O, Maroto-Morales A, Montoro V, et al. 2020, Semen cryodamage in ruminants: Understanding the molecular changes induced by the cryopreservation process to optimize semen quality. International Journal of Molecular Sciences, 21:2781.
Salama MS, Shehabeldin AM, Ashour MA, Al-Ghadi MQ, Marghani BH, Elkon I & Shukry M 2024, Effect of the addition of platelet-rich plasma to Boer buck semen on semen quality and antioxidant activity before and after cryopreservation and in vivo fertility. Small Ruminant Research, 230:107167. ,https://doi.org/ 10.1016/j.smallrumres.2023.107167.
Santiago-Moreno, J, Coloma, MA, Dorado, J, Pulido-Pastor, A, Gómez-Guillamón, F, Salas-Vega, R, Gómez-Brunet, A & López-Sebastián A 2009, Cryopreservation of Spanish ibex (Capra pyrenaica) semen obtained by electroejaculation outside the rutting season. Theriogenology, 71: 1253-1260.
Santiago-Moreno, J, Toledano-Díaz, A, Pulido-Pastor, A, Dorado, J, Gómez-Brunet, A & López-Sebastián, A 2006, Effect of egg yolk concentration on cryopreserving Spanish ibex (Capra pyrenaica) epididymal semenatozoa. Theriogenology, 66: 1219-1226.
Saucedo, L, Rumpel, R, Sobarzo, C, Schreiner, D, Brandes, G, Lustig, L, Vázquez-Levin, MH, Grothe, C & Marín-Briggiler, C 2018, Deficiency of fibroblast growth factor 2 (FGF-2) leads to abnormal semenatogenesis and altered semen physiology. Journal of Cellular Physiology, 233: 640-4651, https://doi.org/ 10.1002/jcp26876.
Selvaraju, S, Binsila, BK, Santhanahalli, SA & Janivara, JP 2016, IGF-1 stabilizes semen membrane proteins to reduce cryoinjury and maintain post-thaw semen motility in buffalo (Bubalus bubalis) semenatozoa. Cryobiology, 73: 55-62. https://doi.org/10.1016/j.cryobiol.2016.05.012.
Semyonov, UA 2010, To characterization of the population of the West Caucasian tur (Capra caucasica Guldenstaedt, Pallas, 1783) in the conditions of mountain ecosystems of the Teber-Dinsky Reserve. Game Management Bulletin, 7: 68-74, [In Russian].
Taher-Mofrad, SMJ, Topraggaleh, TR, Ziarati, N, Bucak, MN, Nouri, M, Seifi, S, Esmaeili, V, Rahimizadeh, P & Shahverdi, A 2020, Knockout serum replacement is an efficient serum substitute for cryopreservation of human semenatozoa. Cryobiology, 92: 208-214. https://doi.org/10.1016/j.cryobiol.2020. 01.013.
Toker, MB, Alcay, S, Gokce, E & Ustuner, B 2016, Cryopreservation of ram semen with antioxidant supplemented soybean lecithin-based extenders and impacts on incubation resilience. Cryobiology, 72: 205-209, https://doi.org/10.1016/j.cryobiol.2016.05.001.
Wahjuningsih, S, Ihsan, M, Siswoyo, DA, Fatmila, DT & Firmawati, A 2021, Extract of Cincau (Mesona palustris B.) supplementation in semen extender improves Boer goat semen cryopreservation. Journal of Advanced Veterinary Research, 11(4): 247-253, URL: https://advetresearch.com/index.php/AVR/article/ view/762.
Weinberg, P 2024, Capra caucasica [homepage on the Internet]. The IUCN Red List of Threatened Species 2020: e.T3794A22143809. [cited 2024 Oct 10]. Available from: https://dx.doi.org/10.2305/ IUCN.UK.2020-2.RLTS.T3794A22143809.en. | ||
|
آمار تعداد مشاهده مقاله: 138 تعداد دریافت فایل اصل مقاله: 158 |
||