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ghasemi, F., Mohebbi, H. (2025). Twelve weeks high intensity interval training independently of hypoxia induced hepatic mitochondria biogenesis in male rats under high fat diet.. Aquatic Physiology and Biotechnology, 14(2), 189-208. doi: 10.22124/jme.2023.26237.339
faeghe ghasemi; Hamid Mohebbi. "Twelve weeks high intensity interval training independently of hypoxia induced hepatic mitochondria biogenesis in male rats under high fat diet.". Aquatic Physiology and Biotechnology, 14, 2, 2025, 189-208. doi: 10.22124/jme.2023.26237.339
ghasemi, F., Mohebbi, H. (2025). 'Twelve weeks high intensity interval training independently of hypoxia induced hepatic mitochondria biogenesis in male rats under high fat diet.', Aquatic Physiology and Biotechnology, 14(2), pp. 189-208. doi: 10.22124/jme.2023.26237.339
ghasemi, F., Mohebbi, H. Twelve weeks high intensity interval training independently of hypoxia induced hepatic mitochondria biogenesis in male rats under high fat diet.. Aquatic Physiology and Biotechnology, 2025; 14(2): 189-208. doi: 10.22124/jme.2023.26237.339

Twelve weeks high intensity interval training independently of hypoxia induced hepatic mitochondria biogenesis in male rats under high fat diet.

سوخت و ساز و فعالیت ورزشی
Volume 14, Issue 2, December 2024, Pages 189-208    PDF (771.68 K)
Document Type: Research Paper I Open Access I Released under (CC BY-NC) license
DOI: 10.22124/jme.2023.26237.339
Authors
faeghe ghasemi; Hamid Mohebbi*
Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Guilan, Rasht, Iran
Abstract
Introduction: Consuming a high-fat diet leads to disruption of liver mitochondrial biogenesis. Training exercise and hypoxia, which are new preventive or therapeutic strategies for obesity-induced Non-alcoholic fatty liver disease (NAFLD), may improve the impaired mitochondrial function. The aim of this study was to determine the effect of nutrition, training and hypoxia on liver mitochondrial biogenesis in male Wistar rats. Methods: Thirty-two male rats (age: 6 weeks old; average weight: 167.25 grams (were randomly divided into four groups of eight including normal diet (ND), high-fat diet (HFD), high-fat diet and training in normoxia (HFD-HIIT) and high-fat diet and training in hypoxia (HFD-HHIIT). After determining the maximum aerobic velocity (MAV) in normoxia and hypoxia-hypobaric, the HIIT protocol was performed for 12 weeks and three sessions per week, which included 3 to 8 bouts 4-minute activity with an intensity of 80 to 93 percent of MAV and 2-minute active rest periods with an intensity of 50 percent of MAV. At the end, the levels of PGC-1α and Tfam genes were measured through RT-PCR and liver fat content was measured through Oil Red staining in liver tissue. Results: Both HFD-HIIT and HFD-HHIIT groups showed a significant increase in the expression of PGC-1α and Tfam genes and a significant decrease in liver fat content compared to the HFD group (p<0.05). However, the HFD+HHIIT group showed a significant increase in Tfam gene expression and a significant decrease in liver fat content compared to the HFD+HIIT group (p<0.05). Conclusions: It seems that HIIT training has been able to increase PGC-1α independent of hypoxia conditions. Whereas, hypoxia with a significant increase in the expression of the Tfam gene, which plays a role in improving the capacity of mitochondrial function, led to a greater decrease in liver fat content than in normoxia conditions.
Keywords
High intensity interval training; Hypoxia; Mitochondrial biogenesis; NAFLD; High Fat Diet
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