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بهبود وضعیت آنتی اکسیدانی، پاسخ های التهابی، فراسنجه های خونی، بیوشیمیایی و عملکرد با مکمل سازی سیاه دانه (Nigella sativa) در جوجه های گوشتی در شرایط تنش اکسیداتیو فیزیولوژیک القایی | ||
تحقیقات تولیدات دامی | ||
دوره 13، شماره 3، آذر 1403، صفحه 33-46 اصل مقاله (524.52 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22124/ar.2024.26665.1813 | ||
نویسندگان | ||
مختار فتحی* 1؛ شهریار سعیدیان2؛ سلیمان مرادی3 | ||
1دانشیار، گروه علوم دامی، دانشگاه پیام نور، تهران، ایران | ||
2دانشیار، گروه زیست شناسی، دانشگاه پیام نور، تهران، ایران | ||
3دانش آموخته کارشناسی ارشد بیوشیمی، گروه زیست شناسی، دانشگاه پیام نور، تهران، ایران | ||
چکیده | ||
این مطالعه بهمنظور بررسی آثار پودر سیاهدانه بر عملکرد رشد و توان آنتیاکسیدانی، پاسخهای التهابی، تغییرات بیوشیمیایی، هماتولوژی در جوجههای گوشتی در شرایط تنش اکسیداتیو القایی با پراکسید هیدروژن انجام شد. تعداد 200 قطعه جوجه گوشتی سویه راس 308 در یک طرح کاملاٌ تصادفی به چهار تیمار، هر تیمار در پنج تکرار دارای 10 جوجه یکروزه اختصاص داده شد. چهار تیمار آزمایشی شامل: 1- تیمار شاهد (تغذیه شده با جیره پایه) و تیمارهای 2، 3 و 4- بهترتیب دریافتکننده جیره پایه بهعلاوه سطوح 5/0، 1 و 5/1 درصد پودر سیاهدانه بودند. برای القای تنش اکسیداتیو، همه پرندگان از روز 14 تا روز 42 آزمایش، آب آّشامیدنی حاوی یک درصد H2O2دریافت کردند. نتایج نشان داد مکملسازی سیاهدانه در جوجههای زیر شرایط تنش اکسیداتیو القایی، بهطور معنیداری باعث بهبود عملکرد رشد، افزایش فعالیت آنزیمهای آنتی اکسیدانی و کاهش MDA در سرم، بافت کبد و طحال شد ( 05/0(P<. علاوه بر این، مکملسازی سیاهدانه بهطور معنیداری سبب کاهش سطح سرمی سیتوکینهای التهابیIL-6 و TNF-α و افزایش سیتوکین ضد التهابی IL-10 (05/0(P< شد. همچنین، مکملسازی سیاهدانه بدون تاثیر بر تعداد گلبول قرمز، درصد هماتوکریت و مقدار هموگلوبین، سبب افزایش شمار گلبول سفید، درصد لنفوسیت و کاهش درصد هتروفیل خون شد (05/0(P<. سطح سرمی تریگلیسیرید، کلسترول و آنزیمهای ALT،AST و ALP نیز بهطور معنیداری تحت تاثیر سیاهدانه کاهش یافت ( 05/0(P<. بهطور کلی، نتایج این تحقیق نشان داد استفاده از سیاهدانه میتواند از راه بهبود توان آنتیاکسیدانی و پاسخهای التهابی، سبب بهبود عملکرد جوجههای گوشتی شود. | ||
کلیدواژهها | ||
آنتی اکسیدان؛ پاسخ های التهابی؛ جوجه های گوشتی؛ فراسنجه های خونی؛ سیاه دانه | ||
مراجع | ||
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