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ریزپوشانی باکتری Pseudomonas fluorescens همراه با نانوذرات اکسید روی/سیلیس و تاثیر آن بر گیاه گوجه فرنگی و برخی از فراسنجه های زیستی Tuta absoluta | ||
تحقیقات آفات گیاهی | ||
دوره 14، شماره 1، خرداد 1403، صفحه 45-60 اصل مقاله (1.21 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22124/iprj.2024.26991.1565 | ||
نویسندگان | ||
الهه تمنادار؛ شهناز شهیدی نوقابی* ؛ روح الله صابری | ||
گروه گیاهپزشکی دانشکده کشاورزی، دانشگاه ولیعصر (عج) رفسنجان، ایران | ||
چکیده | ||
استفاده از ریزپوشانی در فرموله کردن عوامل زیستی مانند باکتریهای آنتاگونیست نقش مهمی در افزایش کارایی و ماندگاری این عوامل در شرایط نامساعد محیطی دارد. در این تحقیق، تاثیر کپسوله کردن باکتری Pseudomonas fluorescens UPF5 با آلژینات-وی پروتئین به همراه نانوذرات اکسید سیلیس و روی بر بعضی عناصر مهم گیاه گوجهفرنگی، تعداد و طول دوره تخمگذاری و طول دوره زندگی شبپره مینوز گوجهفرنگی Tuta absoluta Meyrick (Lepidoptera: Gelechidae) بررسی شد. آزمایشهای گلخانهای در شرایط دمایی 2±27 درجه سلسیوس، رطوبت نسبی 5 ± 60 درصد و دوره نوری 16 ساعت روشنایی و 8 ساعت تاریکی صورت گرفت. تعیین مقدار برخی عناصر مهم در گیاه گوجه فرنگی در چهار تیمار شامل: 1) گیاه تیمار شده با باکتری کپسولهشده، 2) گیاه تیمار شده با نانوذرات اکسید روی+سیلیس، 3) گیاه تیمار شده با باکتری فاقد پوشش و 4) گیاه تیمار شده با آب + آلؤینات + وی پروتئین (شاهد) انجام شد. نتایج نشان داد که تیمار گیاه با باکتری کپسولهشده باعث افزایش به ترتیب 2/10، 8/13 و 6/1 برابری میزان عناصر فسفر، پتاسیم و آهن نسبت به میزان این عناصر در گیاه شاهد شد. علاوه بر این، طول دوره قبل از تخمگذاری بالغ (APOP)، کل دوره قبل از تخمگذاری (TPOP) و طول عمر در حشراتی که روی گیاهان تیمار شده با باکتری کپسولهشده تغذیه شدند بیشتر بود؛ اما تعداد تخم ها در مقایسه با حشرات تغذیه شده از گیاهان شاهد کمتر بود. نتایج نشان داد استفاده از باکتری باعث بهبود رشد گیاه و افزایش مقاومت آن در برابر T. absoluta شد. | ||
کلیدواژهها | ||
باکتری کپسولهشده؛ آفت؛ عناصر ضروری گیاه؛ مقاومت گیاه گوجه فرنگی | ||
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