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بهبود صفات جوانه زنی بذر و رشد گیاهچه در گل همیشهبهار (Calendula officinalis L.) توسط Pseudomonas spp . | ||
علوم و تحقیقات بذر ایران | ||
دوره 10، شماره 3، مهر 1402، صفحه 21-32 اصل مقاله (1.29 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22124/jms.2023.7672 | ||
نویسندگان | ||
پدرام علیموسیزاده1؛ سیدجواد صانعی* 2؛ عبدالحسین طاهری3 | ||
1دانشجوی کارشناسی، گروه گیاهپزشکی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران | ||
2هیات علمی/ گروه گیاه پزشکی دانشگاه علوم کشاورزی و منابع طبیعی گرگان | ||
3دانشیار، گروه گیاهپزشکی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران | ||
چکیده | ||
باکتریهای محرک رشد با استقرار روی ریشه، رشد گیاهان را تحریک میکنند. این آزمایش جهت بررسی چهار استرین از Pseudomonas fluorescens Migula (PF1-4) و شش استرین از Pseudomonas putida (Trevisan) Migula (PP1-6) بر شاخصهای جوانهزنی بذر شامل درصد جوانهزنی، سرعت جوانهزنی، طول و وزن خشک ریشهچه و ساقهچه، شاخص بنیه بذر و گیاهچه و فعالیت آنزیم آلفا- آمیلاز گل همیشه بهار (Calendula officinalis L.) صورت گرفت. تولید سیدروفور و اسید ایندول استیک (IAA) باکتریها در شرایط آزمایشگاه بررسی گردید. در این مطالعه، از سوسپانسیون باکتری با غلظت 109×1 سلول/میلیلیتر برای مایهزنی بذر در قالب طرح کاملا تصادفی استفاده شد. نتایج نشان داد که غلظت متابولیتها بین استرینهای باکتریها بهطور معنیداری متفاوت بود. استرینهای مختلف باکتریهای مورد مطالعه همچنین اثرهای متفاوتی بر شاخصهای جوانهزنی داشتند و برهمکنش گیاه- باکتری بر شاخصهای جوانهزنی مثبت یا بدون اثر بود. بیشینه اثر استرینهای باکتریهای مورد بررسی به باکتریهای PP-1، PP-2، PP-5، PF-1 و PF-2 مربوط بود. تاثیر استرینهای باکتری بر صفات مورد بررسی همبستگی معنیداری با میزان تولید IAA توسط باکتری داشت. بهطور کلی، تاثیر پیشتیمار زیستی با باکتریهای محرک رشد بر شاخصهای جوانهزنی به گونه و استرین باکتری بستگی داشت. نتایج همچنین نقش کلیدی میزان IAA تولید شده توسط باکتری را بر جوانهزنی بذر و تحریک ویژگیهای جوانهزنی نشان داد. | ||
کلیدواژهها | ||
اسید ایندول استیک؛ جوانهزدن بذر؛ سودوموناس؛ گل همیشه بهار | ||
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