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Antibacterial activity of the produced and purified L-glutamate oxidase from Streptomyces sp. | ||
| Caspian Journal of Environmental Sciences | ||
| مقالات آماده انتشار، اصلاح شده برای چاپ، انتشار آنلاین از تاریخ 15 بهمن 1402 اصل مقاله (578.47 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22124/cjes.2024.7503 | ||
| نویسندگان | ||
| Suha Nasser Fadhel* ؛ Ashgan Slman Dawood* ؛ Nahla Kadhim Salman* | ||
| College of Science for Women, University of Baghdad, Baghdad, Iraq | ||
| چکیده | ||
| From Streptomyces species isolated from agricultural soil, an extracellular L-glutamate oxidase was recovered. The Streptomyces bacterial isolates were grown in a selective medium that had L-glutamate as a substrate. The filtrate, which is representative of the crude enzyme, was subsequently obtained by extracting the extracellular enzyme using a cooling centrifugation procedure. L-glutamate oxidase purification operations were then performed, starting with fractionation with 40% ammonium salts and continuing with ion-exchange chromatography and gel filtration with a final 8.25 units/mg and 61.8% yield. Both positive and negative bacteria were sensitive to the pure L-glutamate oxidase's effects. Purified L-glutamate oxidase outperformed other examined bacterial isolates in terms of activity, with MIC values of 32 µg/ml for Klebsiella pneumonia and 64 µg/ml for Staphylococcus aureus and Escherichia coli. Thus, pure L-glutamate oxidase may be viewed as a promising possibility in the rational design of new antibiotics. | ||
| کلیدواژه‌ها | ||
| L-glutamate oxidase؛ Streptomyces؛ Antibacterial activity | ||
| مراجع | ||
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