|تعداد مشاهده مقاله||7,643,035|
|تعداد دریافت فایل اصل مقاله||5,861,202|
Genetic polymorphism of -Lg gene in Iraqi buffalo using Polymerase Chain Reaction – Restriction Fragment Length Polymorphism
|Caspian Journal of Environmental Sciences|
|دوره 21، شماره 2، تیر 2023، صفحه 343-347 اصل مقاله (1.01 M)|
|نوع مقاله: Research Paper|
|شناسه دیجیتال (DOI): 10.22124/cjes.2023.6500|
|Bashar F. Zaqeer* ؛ Muntaha Y. Yousief؛ Salah Mahdi Alsudany؛ Jaafar M. Owaid,؛ Falih Hasan Hamad|
|College of Agriculture, University of Basrah, Basrah, Iraq|
|This study was conducted on 50 buffaloes from Basrah Governorate, Iraq to study the genetic polymorphisms of the β-lactoglobulin gene in the local buffaloes. The lab examinations were conducted in the Genetic Engineering Laboratory, College of Agriculture, University of Basrah. DNA was extracted by kit (PureLink Genomic DNA kits, Invetrogen, USA). The results of this technique showed the appearance of the expected gene bundle of 252 base pairs from all Iraqi buffalo samples, as the primer interacted with all DNA isolates taken from the studied buffalo samples. Digestion technique with restriction enzymes (HaeIII) was used to detect the genetic polymorphisms of β-lactoglobulin gene, exhibiting two bands to all studied buffalo samples. Only the homozygous BB genotype has been shown.|
|Genetic polymorphisms؛ β-lactoglobulin gene؛ PCR technique؛ Iraqi buffalo|
Appel, DJ & Gordon, TR 1995, Intraspecific variation within populations of Fusarium oxysporum based on RFLP analysis of the Intergenic spacer region of the rDNA. Experimental Mycology, 19: 120-128.
Badola, S, Bhattacharya, TK, Biswas, TK, Shivakumar, BM, Kumar, P, Mistra, SS & Sharma, A 2003, Bubaline beta-lactoglobulin gene polymorphism: A contrast from its bovine counterpart. Journal of Buffalo Science, 19: 291-298.
Bruns, TD, White, TJ & Taylor, JW 1991, Fungal Molecular Systematics. Annual Review of Ecology and Systematics, 22: 525-564.
Cattaneo, TMP, Nigro, F & Greppi, GF 1996, Analysis of cow, goat and ewe milk mixtures by capillary electrophoresis: Primary approach. Milchwissenschaft-milk Science International, 51: 616-619, Corpus ID: 99517542.
Chen, W 1992, Restriction fragment length polymorphism in enzymatically amplified ribosomal DNAs of three heterothallic Pythium species. Journal of Phytopathology, 82: 1467-1472.
Chianese, L, Garro, G, Addeo, F, Lopez Galvez, G & Ramos, M 1993, Discovery of an ovine α-s2 casein variant. Journal of Dairy Research, 60: 485-493.
David, VA & Deutch, AH 1992, Detection of bovine α - s1 casein genomic variants using the allele - specific polymerase chain reaction. Animal Genetics, 23: 425-429.
Davies, DT & Law, AJ 1987, Quantitative fraction of casein mixtures y fast protein liquid chromatography. Journal of Dairy Research, 54: 369-376.
Da Xi, R, Shu Ying, M, You liang, Ch, Cai Xia, Z, Xian Wei, l & Jian Xin, L 2011, Genotyping of the k-casein and β-lactoglobulin genes in Chinese Holstein, Jersey and water buffalo by PCR-RFLP. Indian Academy of Sciences, Journal of Genet, 19; 90(1): e1-5.
Di Gregorio, P, Rando, A, Pieragostini, E & Masina, P 1991, DNA polymorphism at the casein loci in sheep. Animal Genetics, 22: 21-30.
Jaayid, TA 2013, Rapid and sensitive identification of horse and donkey meat in Iraqi markets using SSR and PCR-RFLP based on mitochondrial DNA cytochrome b gene. Journal of Agricultural Science and Technology, 3: 11. USA.
Jaayid, TA & Al Allak, ZS 2013, Successful diagnostic of fetal gender using polymerase chain reaction (PCR) of ovine amniotic fluid and embryos produced in vitro. International Journal of Molecular Genetics. 3: 29-37.
Jaayid, TA & Dragh, MA 2013, Genetic biodiversity in buffalo population of Iraq using microsatellites marker. Journal of Agricultural Science and Technology, 4: 297-301.
Jaayid, TA & Saker, DK 2012, Detection of species adulteration in imported meat from farm animals and pork using polymerase chain reaction with species specific repeat (PCR-SSR). Basrah Journal of Agriculture, 25: 151-183.
Jaayid, TA & Saker, DK 2013, Using restriction fragment length polymorphism technique of cytochrome b gene for the identification of meat species and meat products in farm animals. 2nd International Food Safety Conference (IFSAC2013) Food Safety: Critical Dimension of Food Security in Emerging Economies. University of Putra, Malaysia.
Jaayid, TA, Fahid, TA & Jasim, NY 2013, Oocytes extraction from Iraqi local goat ovaries by using aspiration and in vitro fertilization techniques. Basrah Journal of Agriculture, 26: 250-264.
Jaayid, TA, Yousief, Zaqeer, BF & Owaid, JM 2013, Genetic polymorphism of kappa casein (κ-CN) in Iraqi buffalo using polymerase chain reaction-restriction fragment length polymorphism. Journal of Agricultural Science and Technology, 3: 10.
Jaayid, TA, Al Waily, DS & Almubarak, ZHA 2013, Genetic diversity of Fusarium oxysporum f.sp cucumerinum isolates from cucumber: Differentiation by using random amplified polymorphic DNA. Basrah Journal of Agricultural Sciences, 26: 141-158.
Jaayid, TA, Ayied, AY & Zaqeer, BF 2012, Study of b-lactoglobulin genotypes of arabi sheep milk and their relations with some genetic and production parameters. Basrah Journal of Agricultural Sciences, 25: 1-11.
Jaayid, TA, Fahid, TA & Jasim, NY 2013, Oocytes extraction from Iraqi local goat ovaries by using aspiration and in vitro fertilization techniques. Basrah Journal of Agricultural Sciences, 26: 250-264.
Karimi, K, Beigi Nasiri, MT, Fayyazi, J, Mirzadeh, KH & Roushanfekr, H 2009, Allele and genotype frequencies of - lactoglobulin gene in Iranian Najdi cattle and buffalo populations using PCR-RFLP. African Journal of Biotechnology, 8: 3654-3657, http://www.academicjournals.org/AJB.
Ng Kwai Hang, KF 1998, Genetic polymorphism of milk proteins: relationships with production traits, milk composition and technological properties. Canadian Journal of Animal Science, 78: 131-147.
Nualchuen, W, Srisakwattana, K, Chethasing, S, Tasripoo, K, Usawang, S, Hengtrakulsin, R & Kamonpatana, K 2012, RFLP Analysis of beta‐actoglobulin gene in swamp and murrah buffaloes using a single restriction enzyme. Iranian Journal of Applied Animal Science, 2: 301-303.
Patel, AK & Tripathi, VN 1998, Effect of non-genetic factors on economic traits of Surti buffaloes. The Indian Journal of Animal Sciences, 68: 566-569.
Soller, M 1990, Genetic mapping of the bovine genome using deoxyribonucleic acid - level markers to identify loci affecting quantitative traits of economic importance. Journal of Dairy Science, 73: 2628-2646.
Soumi, S, Tarun, KB, Venkatachalaphthy, RT, Pushpendra, K & Sharma, A 2006, Cloning and characterization of αS2-casein gene of Reverine buffalo DNA Seq. 17: 458-464. PMID: 17654008.
Strange, RN 2003, Introduction to plant pathology. Great Britain by TJ International Ltd., Padstow, Cornwall, University College London., 497 p.
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