|تعداد مشاهده مقاله||7,642,223|
|تعداد دریافت فایل اصل مقاله||5,860,432|
Antioxidant activity of Garcinia atroviridis Griff. ex T.Anderson
|Caspian Journal of Environmental Sciences|
|دوره 21، شماره 2، تیر 2023، صفحه 325-331 اصل مقاله (775.45 K)|
|نوع مقاله: Research Paper|
|شناسه دیجیتال (DOI): 10.22124/cjes.2023.6498|
|Sura Maan Salim*|
|Al-Hadba University College, Department of Medical Laboratory Techniques, Iraq|
|This study aimed to investigate and evaluate the amount of antioxidants activity and total phenolic contents of the plant Garcinia atroviridis Griff. ex T.Anderson. Only the leaves and fruit were used for this study. These samples were washed, dried and grinded, then Soxhlet apparatus and methanol were used for extraction, and the end products were used for the study. Two methods were used for this study, 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) assay was used to evaluate the antioxidant activity and the second assay was total phenolic content (TPC) to measure the quantity of phenols in leave and fruit extracts. Four standards were used for DPPH assay including ellagic acid, tannic acid, gallic acid and rutin. Their results were then compared with the two samples results. Twelve concentrations were prepared for the standards and samples. The antioxidant activity was expressed as the efficient concentration needed to scavenge 50% of free radicals. For TPC assay, gallic acid was the standards and six concentrations were prepared for the standards and samples. In both methods, microplate reader machine or spectrophotometer was used to read the absorbance. According to the results of both assays, the fruit extract exhibited higher antioxidant activity and higher total phenolic content compared to the leaves extracts. The efficient concentration of fruit extract was 4 mg mL-1, while in the case of leaves extract, it could not be attained. The mean of gallic acid in 1 mg of fruit extracts was 0.070, while in leaves extract was 0.069. It was also concluded that when the concentration increases, antioxidant activity and total phenolic content elevates as well.|
|Garcinia atroviridis Griff. ex T.Anderson؛ Antioxidant؛ Phenolic؛ 2؛ 2-Diphenyl-1-Picrylhydrazyl (DPPH)|
Aejazuddin, QM 2016, Herbal medicine: A comprehensive review. International Journal of Pharmaceutical Research, 8: 1-5.
Aryal, S, Baniya, MK, Danekhu, K, Kunwar, P, Gurung, R & Koirala, N 2019, Total phenolic content, flavonoid content and antioxidant potential of wild vegetables from Western Nepal. Plants (Basel, Switzerland), 8: 96.
Bayas-Morejón, F, Ramón, R, García-Pazmiño, M, Mite-Cárdenas, G 2020, Antibacterial and antioxidant effect of natural extracts from Baccharis latifolia (chilca), Caspian Journal of Environmental Sciences, 18: 489-493.
Espirito Santo, BLSd, Santana, LF, Kato Junior, WH de Araújo, FdO, Bogo, D, Freitas, KdC, Guimarães, RdCA, Hiane, PA, Pott, A, Filiú, WFdO, Arakaki Asato, M, Figueiredo, PdO, Bastos, PRHdO 2020, Medicinal potential of Garcinia species and their compounds. Molecules, 25: 4513.
Gangwar, M, Gautam, MK, Sharma, AM, Tripathi, YB, Goel, RK, Nath, G 2014, Antioxidant capacity and radical scavenging effect of polyphenol rich Mallotus philippenensis fruit extract on human erythrocytes: An in vitro study. The Scientific World Journal, 279451, DOI: 10.1155/2014/279451
Gupta, A, Kumar, R, Ganguly, R, Singh, AK, Rana, HK & Pandey, AK 2020, Antioxidant, anti-inflammatory and hepatoprotective activities of Terminalia bellirica and its bioactive component ellagic acid against diclofenac induced oxidative stress and hepatotoxicity. Toxicology reports, 8: 44-52.
Hafiza, N, Nabil, S, Muhammad, F & James SOM 2017, Measurement of total phenolic content and antioxidant activity of aerial parts of medicinal plant Coronopus didymus. Asian Pacific Journal of Tropical Medicine, 10: 792-801.
Hatami, T, Emami, SA, Miraghaee, SS & Mojarrab, M 2014, Total phenolic contents and antioxidant activities of different extracts and fractions from the aerial parts of Artemisia biennis Willd. Iranian Journal of Pharmaceutical Research: IJPR, 13: 551-559.
Kedare, SB & Singh, RP 2011, Genesis and development of DPPH method of antioxidant assay. Journal of Food Science and Technology, 48: 412-422.
Khademian Amiri, SA, Aghajanzadeh, T, Jafari, N, Mahmoudi, M 2022, Antioxidative compounds, enzymes activity and nutrient elements in Stachys byzantina are altered by climate conditions not by soil parameters. Caspian Journal of Environmental Sciences, 20: 1099-1115.
Krishnaraju, AV, Rao, TVN, Sundararaju, D, Vanisree, M, Tsay, H & Subbaraju, GV 2005, Assessment of bioactivity of Indian Medicinal plants using Brine shrimp (Artemia salina) lethality assay. International Journal of Applied Sciences and Engineering, 3: 125- 134.
Kumar, N & Goel, N 2019, Phenolic acids: Natural versatile molecules with promising therapeutic applications. Biotechnology reports (Amsterdam, Netherlands), 24: e00370.
Kumar, S, Sandhir, R & Ojha, S 2014, Evaluation of antioxidant activity and total phenol in different varieties of Lantana camara leaves. BMC Research Notes, 7: 560.
Lautié, E, Russo, O Ducrot, P & Boutin, JA 2020, Unraveling plant natural chemical diversity for drug discovery purposes. Frontiers in Pharmacology, 11: 1663-9812.
Le, K, Chiu, F & NgK 2007, Identification and quantification of antioxidants in Fructus lycii. Food Chemistry, 105: 353-363.
Lourenço, SC, Moldão Martins, M & Alves, VD 2019, Antioxidants of natural plant origins: From sources to food industry applications. Molecules (Basel, Switzerland), 24: 4132.
Manessis, G, Kalogianni, AI, Lazou, T, Moschovas, M, Bossis, I & Gelasakis, AI 2020, Plant-derived natural antioxidants in meat and meat products. Antioxidants, 9: 1215.
Mohyelden, AO, Ghada IM & Shaimaa SS 2021, Correlation between total phenols content, antioxidant power and cytotoxicity. Biointerface Research in Applied Chemistry, 11: 10640-10653.
Moragot, C, Pitaksit, C, Patcharaporn, P, Chantanapa, C, Sutida, W, Jiraphat, N, Jitbanjong, T & Anchalee, C 2020, Antioxidant and tyrosinase inhibitory properties of an aqueous extract of Garcinia atroviridis Griff. ex. T. Anderson Fruit Pericarps. Pharmacognosy Journal, 12: 71-79.
Nirmala, P, Pramod, KJ, Pankaj, PR & Sangeeta, R 2020, Total phenolic, flavonoid contents, and antioxidant activities of fruit, seed, and bark extracts of Zanthoxylum armatum DC. The Scientific World Journal, 8780704-7, DOI: 10.1155/2020/8780704.
Olszowy Tomczyk, M 2021, How to express the antioxidant properties of substances properly? Chemical Papers, 75: 6157-6167.
Panche, AN, Diwan, AD & Chandra, SR 2016, Flavonoids: An overview. Journal of Nutritional Science, 5: e47.
Rahman, MM, Islam, MB, Biswas, M & Khurshid Alam, AHM 2015, In vitro antioxidant and free radical scavenging activity of different parts of Tabebuia pallida growing in Bangladesh. BMC Research Notes, 8: 621.
Santos Sánchez, NF, Salas Coronado, R, Villanueva Cañongo, C & Hernández Carlos, B 2019, Antioxidant compounds and their antioxidant mechanism. In: Antioxidants, IntechOpen, DOI: 10.5772/Intech open.85270
Tavares, WR, Barreto, M & Seca, A 2020, Uncharted source of medicinal products: the case of the Hedychium genus. Medicines (Basel, Switzerland), 7: 23.
Zhang, RF, Zhang, FX, Zhang, MW, Wei, ZC, Yang, CY, Zhang, Y, et al. 2011, Phenolic composition and antioxidant activity in seed coats of 60 Chinese black soybean (Glycine max L. Merr.) varieties. Journal of Agriculture and Food Chemistry, 59: 5935- 5944.
تعداد مشاهده مقاله: 154
تعداد دریافت فایل اصل مقاله: 271