|تعداد مشاهده مقاله||7,656,725|
|تعداد دریافت فایل اصل مقاله||5,868,734|
Efficacy of nanoparticle zinc oxide in the resistance of fungus Rhizoctonia solani causing black scurf disease in local potatoes
|Caspian Journal of Environmental Sciences|
|دوره 21، شماره 1، فروردین 2023، صفحه 95-103 اصل مقاله (897.31 K)|
|نوع مقاله: Research Paper|
|شناسه دیجیتال (DOI): 10.22124/cjes.2023.6199|
|Wafaa J.R. Al-Zaidi1؛ Amna M. Ali1؛ Thamer A.A. Muhsen* 2|
|1Department of Sciences, College of Basic Education, Al-Mustansiriyah University, Iraq|
|2Department of Biology, College of Education for Pure Science Ibn-Al-Haitham, University of Baghdad, Iraq|
|This study was conducted to isolate and purify the causative agent of Black Scurf disease on potato crop and to test the efficiency of three concentrations (1, 2 and 3%) of zinc oxide nanoparticles (ZnO-PNs) and zinc oxide (ZnO) in controlling the fungus Rhizoctonia solani. The results of morphology and then molecular diagnosis by PCR technology showed that isolate R22 represents the fungus Rhizoctonia solani, which is the cause of potato black scurf disease. The isolate was registered in the gene bank under accession number OM83978. According to the results of laboratory inhibition exhibited that 3% nano-zinc oxide leads to the highest inhibition rate (85.9%) compared to the control group. Also, 3% zinc oxide showed inhibition rate of 43.71% compared to the control. The results of the pot treatments test showed that all of them had an effect in reducing the infection rate (%) and the severity of infection with the fungus R22. So that, the nano-zinc oxide treatment recorded the lowest rate (%) and severity of infection (11% and 4.73% respectively), followed by the fungicide (Ethidium bromide), which scored 22.22% and 18.33% respectively, while in the case of zinc oxide, we recorded 33.33% and 19.03%, respectively, compared to the control group (plant with R22). The results of the study of growth parameters (plant height, fresh weight, dry weight and number of leaves) also exhibited that the nano-zinc oxide treatment recorded a positive increase in growth parameters that amounted to 54, 24.33, 7.33 and 99.33% compared to the control group.|
|Nanoparticle؛ Zinc oxide؛ Rhizoctonia solani؛ Potatoes؛ plants|
Abd Elsalam, KA, Vasil’kov, AY, Said Galiev, EE, Rubina, MS, Khokhlov, AR, Naumkin, AV & Alghuthaymi, MA 2018, Bimetallic blends and chitosan nanocomposites: novel antifungal agents against cotton seedling damping-off. European Journal of Plant Pathology, 151: 57-72.
Abd-Elsalam, KA, Vasil’kov, AY, Said Galiev, EE, Rubina, MS, Khokhlov, AR, Naumkin, AV & Alghuthaymi, MA 2018, Bimetallic blends and chitosan nanocomposites: novel antifungal agents against cotton seedling damping-off. European Journal of Plant Pathology, 151: 57-72.
Akintelu, SA & Folorunso, AS 2020, A review on green synthesis of zinc oxide nanoparticles using plant extracts and its biomedical applications. BioNanoScience, 10: 848-863.
Al Dhabaan, FA, Shoala, T, Ali, AA, Alaa, M, Abd Elsalam, K & Abd Elsalam, K 2017, Chemically-produced copper, zinc nanoparticles and chitosan-bimetallic nanocomposites and their antifungal activity against three phytopathogenic fungi. International Journal of Agricultural Technology, 13: 753-769.
Al Dulaimi, SI & Hussein, HZ 2019, Molecular diagnosis of Fusarium oxysporum lycopersici and proving its pathogenic capacity on the incidence of fusarium wilt disease.
AL-Isawi, HIN 2022, Effects of applying cold and hot aqueous extracts of ginger to control onion rot disease caused by Aspergillus niger. Caspian Journal of Environmental Sciences, 20: 611-616
Arciniegas Grijalba, PA, Patiño Portela, MC, Mosquera Sánchez, LP, Guerrero Vargas, JA & Rodríguez Páez, J E 2017, ZnO nanoparticles (ZnO-NPs) and their antifungal activity against coffee fungus Erythricium salmonicolor. Applied Nanoscience, 7: 225-241.
Betancourth García, CA, Castro Caicedo, BL, Quiroz Ojeda, C, Sañudo Sotelo, B, Florez Casanova, C & Salazar González, C 2021, Morphology and pathogenicity of Rhizoctonia solani kühn associated with potato black scurf in nariño (Colombia). Revista Colombiana de Ciencias Hortícolas, 15: e11821-e11823.
Ferrucho, RL, Cifuentes, JM, Ceresini, P & García Domínguez, C 2012, Rhizoctonia solani AG-3PT is the major pathogen associated with potato stem canker and black scurf in Colombia. Agronomía Colombiana, 30: 204-213.
Haider, AA & Hussein, HZ 2022, Efficiency of biologically and locally manufactured silver nanoparticles from Aspergillus niger in preventing Aspergillus flavus to produce aflatoxin B1 on the stored maize grains. Caspian Journal of Environmental Sciences, 20: 765-773.
Hall, BD, Zanchet, D & Ugarte, D 2000, Estimating nanoparticle size from diffraction measurements. Journal of Applied Crystallography, 33: 1335-1341.
Hassan, MS, Al Samarai, IK & Kadhum, AA 2012, Inducing resistance against Rhizoctonia solani using Azotobacter chroocoum and Trichoderma harazianum in potato plants. The Iraqi Journal of Agricultural Sciences, 43: 1-8.
Hussein, HZ, Abdul Karim, EK & Mutar, SS 2017, Possibility of Using Nanoparticles (ZnNPs, MgO-NPs) in Keeping Cucurbit Fruit from Infection by Pythium aphanidermatum. International Journal of Science and Research, 6: 837-839.
Juber, KS & Hasson, IK 2012, Identifying anastomosis groups and virulence of the isolates of Rhizoctonia solani on potato. The Iraqi Journal of Agricultural Sciences, 34: 74-86.
Kamali Omidi, T, Khorgami, A & Taleshi, K 2022, Effect of foliar application of humic acid levels and nano-fertilizer application on some quantitative and qualitative traits of pumpkin (Cucurbita pepo L.) in climatic conditions of Khorramabad area, Iran. Caspian Journal of Environmental Sciences, 20: 467-476.
Larkin, RP & Griffin, TS 2007, Control of soilborne potato diseases using Brassica green manures. Crop Protection, 26: 1067-1077.
Malandrakis, AA, Kavroulakis, N & Chrysikopoulos, CV 2019, Use of copper, silver and zinc nanoparticles against foliar and soil-borne plant pathogens. Science of the Total Environment, 670: 292-299.
Mckinney, HH 1923, Influence of temperature and moisture on infection of wheat seedling by Helminthosporium sativum. Journal of Agricultural Research, 26: 195-217.
Misawa, T & Kurose, D 2019, Anastomosis group and subgroup identification of Rhizoctonia solani strains deposited in NARO Genebank, Japan. Journal of General Plant Pathology, 85: 282-294.Doi: 10.1007/s10327-019-00848-8.
Muhsen, TA 2011, The Effect of essential oil extracted from yellow peel of Citrus aurantium L. on growth of some fungi. Ibn AL-Haitham Journal for Pure and Applied Sciences, 24.
Muhsen,TA & Ali, BZ 2015, Evaluation of the efficacy of arbuscular mycorrhizal fungi in enhancing resistance of Lycopersicon esculentum roots against Fusarium oxysporum wilt disease. Ibn AL-Haitham Journal for Pure and Applied Sciences, 28: 292-306.
Ouda, SM 2014, Antifungal activity of silver and copper nanoparticles on two plant pathogens, Alternaria alternata and Botrytis cinerea. Research Journal of Microbiology, 9:34. 63, 651-666. DOI: 10.1111/ppa.12139.
Parizi, MA, Moradpour, Y, Roostaei, A, Khani, M, Negahdari, M & Rahimi, G 2014, Evaluation of the antifungal effect of magnesium oxide nanoparticles on Fusarium oxysporum f. sp. lycopersici, pathogenic agent of tomato. European Journal of Experimental Biology, 4: 151-156.
Perelshtein, I, Applerot, G, Perkas, N, Wehrschuetz Sigl, E, Hasmann, A, Gübitz, G & Gedanken, A 2009, CuO–cotton nanocomposite: Formation, morphology, and antibacterial activity. Surface and Coatings Technology, 204: 54-57.
Pitt, JI & Ailsa, DH 2009, Fungi and Food Spoilage. 3rd Edition. Springer Dordrecht Heidelberg, London, New York. 518 p.
Rahul, SN, Khilari, K, Jain, SK, Dohrey, RK & Dwivedi, A 2016, Management of black scurf of potato caused by Rhizoctonia solani with organic amendments and their effect on different parameter of potato crop. Journal of Pure and Applied Microbiology, 10: 2433-2438.
Reddy, PR, Jayarambabu, N, Somasai, AK, Rao, KV & Aparna, Y 2016, Structural and morphological studies of zno-CeO2 nanocomposites. Materials Today: Proceedings, 3: 4146-4150.
Ruffo Roberto, S, Youssef, K, Hashim, AF & Ippolito, A 2019, Nanomaterials as alternative control means against postharvest diseases in fruit crops. Nanomaterials, 9: 1752.
Samson, R, Hoekstra, E & Van Oorschot, CAN 1984, Introduction to Food Borne Fungi. Baarn, Central BureauVoor Schimmel Cultures, pp. 40-41.
Sardella, D, Gatt, R & Valdramidis, VP 2018, Assessing the efficacy of zinc oxide nanoparticles against Penicillium expansum by automated turbidimetric analysis. Mycology, 9: 43-48.
SAS 2012, Statistical analysis system, user’s guide. Statistical. Version 9. 1st ed. SAS Institute Inc. Cary, NC, USA.
Seleiman, MF, Almutairi, KF, Alotaibi, M, Shami, A, Alhammad, BA & Battaglia, ML 2021, Nano-fertilization as an emerging fertilization technique: why can modern agriculture benefit from its use. Plants, 10: 2.
Siddique, MAB, Fateh, FS, Rehman, ZU & Saleem, H 2020, Black scurf of potato disease prevalence in the markets of federal capital territory, Pakistan. Pakistan Journal of Agricultural Research, 33: 440-444.
Subramanian, KS, Manikandan, A, Thirunavukkarasu, M & Rahale, CS 2015, Nano-fertilizers for balanced crop nutrition. In Nanotechnologies in food and agriculture. Springer, Cham, pp. 69-80.
Xu, L, Zhu, Z & Sun, DW 2021, Bioinspired nanomodification strategies: moving from chemical-based agro-systems to sustainable agriculture. ACS nano, 15: 12655-12686.
Zahmatkesh, A, Karimzadeh, K, Faridnia, M 2020, Effect of dietary selenium nanoparticles and chitosan oligosaccharide on biochemical parameters of Caspian roach (Rutilus caspicus) under malathion stress. Caspian Journal of Environmental Sciences, 18: 59-71
تعداد مشاهده مقاله: 642
تعداد دریافت فایل اصل مقاله: 436