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Phenotypic and molecular diagnosis of Fusarium oxysporum and Macrophomina phaseolina isolated from cucumis melon roots | ||
Caspian Journal of Environmental Sciences | ||
دوره 21، شماره 1، فروردین 2023، صفحه 75-84 اصل مقاله (1.21 M) | ||
نوع مقاله: Research Paper | ||
شناسه دیجیتال (DOI): 10.22124/cjes.2023.6197 | ||
نویسندگان | ||
A. M. A. Al-Enezi* ؛ D. S. Jamil | ||
Plant Protection Department, College of Agricultural Engineering Sciences, University of Baghdad, Iraq | ||
چکیده | ||
This study aimed to isolate and diagnose some fungi escorted with the Cucumis melon roots plants both morphologically and molecularly. The results of isolation and diagnosis revealed 57 isolates from the sampling areas, which included Anbar, Abu Ghraib and Al-Yusufiya, which were represented by Macrophomina phaseolina, Fusarium spp., and Rhizoctoinia solani. The fungus Macrophomina phaseolina recorded the most significant values by giving the highest frequent reached 27.08%, followed by Fusarium spp. (20.83%), and Rhizoctoinia solani (11.45%). The pathogenicity results of the purified isolates of Fusarium spp., and Macrophomina phaseolina revealed that all isolates were significantly reduced the germination rate. However, the isolate F18 was the most intense isolate in reducing the germination rate (%), which were recorded zero germination rate on Cucumis melon and radish seeds. The isolate F12 revealed a reduction in the germination rate of Cucumis melon and radish seeds (0.67%), followed by the isolate of F. solani (F16; 10%) on Cucumis melon seeds. The isolates of M. phaseolina revealed a decrement in the germination rate, so that the isolate M1 recorded a zero-germination rate on Cucumis melon and radish seeds, followed by M15 with a germination rate of 3.33% on Cucumis melon seeds, while M7 exhibited a germination rate of 3.33% on radish seeds. The DNA electrophoresis results of the pathogenicity tested isolates of radish seeds and Cucumis melon seeds were the most pathogenic using the specialized initiator ITS4 ̸ ITS1, as it recorded the bundles of molecular weight reached 519 for Fusarium oxyspoum and 560 for Macrophomina phaseolina. These were compared with the gene bank for the presence of a high corresponding for the pathogenic fungi, which were deposited in the gene bank by accession numbers of OK560451, OK560452 and also OK560453 and OK560454, indicating by the codes of MP-Iraq1, MP-Iraq2 and also FS- Iraq1 and FS-Iraq2. | ||
کلیدواژهها | ||
Cucumis melon؛ Fusarium oxyspoum؛ Macrophomina phaseolina | ||
مراجع | ||
AOAD 2016, Arab Agricultural Statistics Yearbook. The Arab Organization for Agricultural Development, 36 p.
Abdul Jaleel, C, Gopi, R, Chang Xing, Z, Azooz, MM & Panneerselvan, R 2016, Plant growth regulators and fungicides alters growth characteristics in Catharanthus roseus, comparative study. Global Journal, Molecular Science, 3: 93 - 99.
Aegerter, BJ, Gordon, TR & Davis, RM 2000, Occurrence and Pathogenicity of fungi associated with melon root rot and vine decline in California. Plant Disease, 84: 224-230.
Al-Abbasi, SHA, Al-Majmaei, AAM, Al-Naqib, ATH, Hameed, AM, AL-Samarraie, MQ & Altaef, AH 2021, Isolation and identification of some fungi from rhizospheric soils of some wild plants at Samarra University, Iraq. Caspian Journal of Environmental Sciences, 19: 829-839
Al-Ani, NMS 1988, Morphological and physiological studies of the fungus Macrophomina phaseolina that causes charcoal rot. MSc. Dissertation, College of Agriculture. University of Baghdad, 98 p.
Al- Ethawi, MB & Al-Taae, HH 2022, First record at molecular level for Rhizoctonia solani causing Rot Root on Aleo vera plants in Iraq. Caspian Journal of Environmental Sciences, 20: 955-965.
Al-Khafajy, RAD, AL Taey, KA & AL Mohammed, MH 2020, The impact of water quality, bio fertilizers and selenium spraying on some vegetative and flowering growth parameters of Calendula officinalis L. under Salinity Stress. International Journal of Agricultural and Statistical Sciences, 16: 1175-1180.
Ben, SI, Correia, KC, Boughalleb, N, Michereff, SJ, Leon, M, Abad Campos, P, Garcia Jimenz, J & Armengol, J 2013, M. eutypoides, a cause of root rot and vine decline in Tunisia, and evidence that M. cannonballus and M. eutypoides are distinct species. Plant Disease, 97: 737-743.
Bhatia, A., Kumar, A, Das, TK, Singh, J, Jain, N & Pathak, H 2013, Methane and nitrous oxide emissions from soils under direct seeded rice. International Journal of Agricultural and Statistical Sciences, 9: 729-736.
Booth, C 1977, Fusarium laboratory guide to the identification of the major species. Commonwealth Mycological Institute Kew, Surrey, England. 58 p.
Bruce, RJ & West, CA 1989, Elicitation of Lignin biosynthesis and isoperoxidase activity by pectic fragments in suspension cultures of castor bean. Plant physiology, 91: 889-897.
EL Deeb, AA, Elian, MI, Hilal, AA & Ali, AA 1987, Sesame root-rot and wilt disease and methods to reduce their damage in Egypt. Zagazig. Journal of Agricultural Research, 14: 437-481.
Gordon, TR, Okamoto, D & Jacobson, DJ 1989, Colonization of muskmelon and non-host crops by Fusarium oxysporum f. sp. melonis and other species of Fusarium. Phytopathology, 79:1095-1100.
Hamza, H, Belkadhi, MS, Triki, MA & Zouba, A 2007, Morphology and biological studies of Monosporascus cannonballus, the cause of root rot and vine decline of melon in Southern Tunisia. Tunisian Journal of Plant Protection, 2: 71-77.
Haider, AA, Hussein, HZ 2022, Efficiency of biologically and locally manufactured silver nanoparticles from Aspergillus niger in preventing Aspergillus flavus to produce aflatoxin B1 on the stored maize grains. Caspian Journal of Environmental Sciences, 20: 765-773.
Hassan, AA 2001, Cucurbits, melon, cantaloupe, cantaloupe, and cucumber. Physiological production technology. Agricultural practices. Arab House for Publishing and Distribution. Cairo. The Egyptian Arabic Republic.
Hassan, MS 1996, Studies on cowpea root rot disease caused by the fungus Macrophomina Phaseolina. Al-Qadisiyah Magazine. 33: 122-133
Hibbett, DS et al. 2007, A higher-level phylogenetic classification of the Fungi Mycological. Research, 111: 509-547.
Hirano, Y & Arie, T 2006: PCR-based differentiation of Fusarium oxysporum f. sp. lycopersici and radicis-lycopersici and races of F. oxysporum f. sp. lycopersici. Journal of General Plant Pathology, 72: 273-283.
Holliday, P & Punithalinem, E 1970, Macrophomina phaseolina. CMI Descriptions of Pathogenic Fungi and Bacteria. CMI, England, 275 p.
Ismail, IA, Hessan, AKh & Abboud, IJ 2009, First record of charcoal rot disease (Macrophomina) on Cucumis melon. Iraqi Journal of Agricultural Sciences, 40: 79-81.
Kubota, C, Olsen, M & McClure, MA 2007, Introduction of grafting as a new IPM tool in Arizona melon production. Department of plant Sciences. University of Arizona. 144 p.
Kumar, S, Stecher, G, Li, M, Knyaz, C & Tamura, K 2018, MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35: 1547-1549.
Leisle, JF & Summerell, BA 2006, The fusarium laboratory manual. Photographs by Suzanne Bullock. 405 p.
Lozovaya, VV, Lygin, AV, Zernova, OV, Li, S, Wind Holm, JM & Hartman, GL 2006, Lignin degradation by Fusarium solani. Plant Disease, 9:77-82.
Mas, P, Molot, PM & Risser, G 1981, Fusarium wilt of muskmelon. In: Fusarium disease, biology and taxonomy, PE Nelson, TA Toussoun, RJ Cook, (Eds.), Pennsylvania State University Press, University Park, PA, USA. pp. 169-177.
Maslienko, LV, Voronkova, AK, Datsenko, LA & Efimtseva, EA 2021, Antagonistic effect of the promising fungal producer strain of microbiopreparation T-1 Trichoderma sp. on oil flax Fusarium blight. Caspian Journal of Environmental Sciences, 19: 883-890
Muhire, BM, Varsani, A & Martin, DP 2014, SDT: A virus classification tool based on pairwise sequence alignment and identity calculation. PLoS ONE, 9: 1-8.
Pivonia, S, Cohen, R, Kafkafi, U, Ben ze’ev, IS & Katan, J 1997, Sudden with of melons in southern Israel: Fungal agents and relationship with plant development. Plant Disease, 81: 1264-1268.
Risser, G, Banihashimi, Z & Davis, DW 1976, A proposed nomenclature of Fusarium oxysporum f. sp. melonis races and resistance genes in Cucumis melon. Journal of Phytopathology, 66: 1105-1106.
Singh, AK, Bhatt, BP, Sundaram, PK, Naresh, C, Bharati, RC & Patel, SK 2012, Faba bean (Vicia faba L.) phenology and performance in response to its seed size class and planting depth. International Journal of Agricultural and Statistical Sciences, 8: 97-109.
Singh, A, Varma, R & Shanmugan, V 2006, Extracellular chitinases of fluorescent pseudomonades antifungal to Fusarium oxysporum f. SP dianth. Causing carnation with Cur. Microbiology, 52: 310-316.
Taubenhaus, JJ & Ezekiel, WN 1933, Anew Charcoal rot of Cantaloupes. Tex. Agric. Exp. Stn. Annual Reports in Medicinal Chemistry, 45: 74 - 75.
Toghueo, RMK 2019, Bioprospecting endophytic fungi from Fusarium genus as sources of bioactive metabolites. Mycology, 1-21.
Villeneuve, F & Maignien, G 2008, Status of soil-borne phytosanitary problems encountered in melon (Cucumis melo) in the main Producing regions of France. In: PM, Cucurbitaceae 2008 Proceeding of The IXth EU CARPTA Meeting on Genetics and Breeding of Cucurbitaceae. pp. 407-414
Yang, B, Young Hong, G, Chunling, W & Xuewen, L 2007, Melon production in china. Acta Horticultural (ISHS), 731: 493-500.
Zheng, N, Zhang, LP, GE, FY, Huang, WK, Kong, LA, Peng, DL & Liu, SM 2018, Conidia of one Fusarium solani isolate from a soybean-production field enable to be virulent to soybean and make soybean seedlings wilted. Journal of Integrative Agriculture. 17: 2042-2053. | ||
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