پیوندهای مفید
آمار
| تعداد نشریات | 31 |
| تعداد شماره‌ها | 710 |
| تعداد مقالات | 6,695 |
| تعداد مشاهده مقاله | 9,268,457 |
| تعداد دریافت فایل اصل مقاله | 6,372,175 |
Association between the CRISPR-associated cas genes and beta-lactamase genes (blaTEM, blaSHV, and blaCTX-M) in uropathogenic Escherichia coli (UPEC) | ||
| Caspian Journal of Environmental Sciences | ||
| مقالات آماده انتشار، اصلاح شده برای چاپ، انتشار آنلاین از تاریخ 10 بهمن 1401 اصل مقاله (1.1 M) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22124/cjes.2023.6185 | ||
| نویسندگان | ||
| Neda Merikhi1؛ Jamileh Nowroozi* 1؛ Ali Nazemi2؛ Mehrdad Hashemi3؛ Robab Rafiei Tabatabaei1 | ||
| 1Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran | ||
| 2Department of Biology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran | ||
| 3Department of genetics, Faculty of advanced sciences and technology, Islamic Azad University, Tehran medical sciences branch, Tehran, Iran | ||
| چکیده | ||
| The purpose of this work was to assess the prevalence of cas genes (cas 1, 2, 3, 5 and 7) in UPEC isolates, as well as the connection between CRISPR-associated cas genes and beta-lactamase genes (blaTEM, blaSHV, and blaCTX-M). The Kirby–Bauer disk diffusion technique was used to assess the susceptibility of isolates to targeted antibiotics. For isolates that were resistant to at least one of the third-generation of cephalosporins in the antibiotic susceptibility test, the combined disk test was conducted. The blaTEM, blaSHV, blaCTX-M, and cas genes (cas 1, 2, 3, 5 and 7) were identified molecularly using the polymerase chain reaction technique. A total of 106 uropathogenic Escherichia coli isolates were identified from 437 positive urine culture samples. Overall, 64.1 % of isolates harbored blaTEM genes. Nevertheless, 46.2 % and 35.9 % of isolates, respectively, carried blaSHV and blaCTX-M. The cas 1, 2, 3, 5 and 7 genes were detected in 96.2 %, 48.1 %, 60.4 %, 65.1%, and 50% of isolates, respectively. We discovered a statistically significant association between the presence of cas2 and the presence of blaSHV (p-value = 0.01) and blaTEM (p-value = 0.02) viruses. We observed a statistically significant association between the presence of at least three cas genes and the presence of blaSHV (p-value = 0.001) gene. At the end, we detected a statistically significance between simultaneous presence of at least five cas genes and simultaneous presence of beta-lactamase genes (blaTEM, blaSHV and blaCTX-M; Pv: 0.01). In general, there is an inverse relationship between the presence of cas2 gene and the presence of blaSHV (Pv: 0.01) and (Pv: 0.02) blaTEM and also between the presence of blaSHV gene alone and the simultaneous presence of blaSHV and blaTEM genes with the simultaneous presence of at least three cas genes in UPEC strains. In general, there is an inverse relationship between the presence of cas2 gene and the presence of blaSHV (Pv: 0.01) and (Pv: 0.02) blaTEM and also between the presence of blaSHV gene alone and the simultaneous presence of blaSHV and blaTEM genes with the simultaneous presence of at least three cas genes in UPEC strains.In general, an inverse relationship between genes cas2 and the blaSHV (Pv: 0.01) and (Pv: 0.02) blaTEM as well as the presence of blaSHV alone and the simultaneous presence of genes blaSHV and blaTEM with the simultaneous presence of at least three genes cas strains UPECIn general, an inverse relationship between genes cas2 and the blaSHV (Pv: 0.01) and (Pv: 0.02) blaTEM as well as the presence of blaSHV alone and the simultaneous presence of genes blaSHV and blaTEM with the simultaneous presence of at least three genes cas strains UPEC. The results of this study indicated that the presence of cas genes linked with the CRISPR system may help lower the presence of beta-lactamase genes (blaTEM, blaSHV, and blaCTX-M) in UPEC isolates. | ||
| کلیدواژه‌ها | ||
| CRISPR-associated (cas) genes؛ Uropathogenic Escherichia coli؛ UPEC؛ ESBL؛ Beta-lactamase genes | ||
| مراجع | ||
|
Almendros, C, Nobrega, FL, Mckenzie, RE & Brouns, SJJ 2019, Cas4–Cas1 fusions drive efficient PAM selection and control CRISPR adaptation. Nucleic Acids Research, 47: 5223-5230.
Aydin, S, Personne, Y, Newire, E, Laverick, R, Russell, O, Roberts, AP & Enne, VI 2017, Presence of Type IF CRISPR/Cas systems is associated with antimicrobial susceptibility in Escherichia coli. Journal of Antimicrobial Chemotherapy, 72: 2213-2218.
Barrangou, R 2015, Diversity of CRISPR-Cas immune systems and molecular machines. Genome Biology, 16: 247.
Bayas-Morejón, F, Ramón, R, García-Pazmiño, M, Mite-Cárdenas, G 2020, Antibacterial and antioxidant effect of natural extracts from Baccharis latifolia (chilca), Caspian Journal of Environmental Sciences, 18: 489-493.
Bartoletti, R, Cai, T, Wagenlehner, FM, Naber, K & Johansen, TEB 2016, Treatment of urinary tract infections and antibiotic stewardship. European Urology Supplements, 15: 81-87.
Bikard, D, Hatoum-Aslan, A, Mucida, D & Marraffini, LA 2012, CRISPR interference can prevent natural transformation and virulence acquisition during in vivo bacterial infection. Cell Host Microbe, 12: 177-86.
Brouns, SJ, Jore, MM, Lundgren, M, Westra, ER, Slijkhuis, RJ, Snijders, AP, Dickman, MJ, Makarova, KS, Koonin, EV & Van DerOost, J 2008, Small CRISPR RNAs guide antiviral defense in prokaryotes. Science, 321: 960-964.
Burley, KM & Sedgley, CM 2012, CRISPR-Cas, a prokaryotic adaptive immune system, in endodontic, oral, and multidrug-resistant hospital-acquired Enterococcus faecalis. Journal of Endod, 38: 1511-1515.
Burstein, D, Sun, CL, Brown, CT, Sharon, I, Anantharaman, K, Probst, AJ, Thomas, BC & Banfield, JF 2016, Major bacterial lineages are essentially devoid of CRISPR-Cas viral defence systems. Nature Communications, 7: 1-8.
Bush, K 2018, Past and present perspectives on β-lactamases. Antimicrobial Agents and Chemotherapy, 62.
Cady, KC, White, AS, Hammond, JH, Abendroth, MD, Karthikeyan, RS, Lalitha, P, Zegans, ME & O'Toole, GA 2011, Prevalence, conservation and functional analysis of Yersinia and Escherichia CRISPR regions in clinical Pseudomonas aeruginosa isolates. Microbiology, 157: 430-7.
Chen, H, Cohen, P & Chen, S 2010, How big is a big odds ratio? Interpreting the magnitudes of odds ratios in epidemiological studies. Communications in Statistics—simulation and Computation®, 39: 860-864.
D’andrea, MM, Arena, F, Pallecchi, L & Rossolini, GM 2013, CTX-M-type β-lactamases: a successful story of antibiotic resistance. International Journal of Medical Microbiology, 303: 305-317.
Dehbanipour, R, Rastaghi, S, Sedighi, M, Maleki, N & Faghri, J 2016, High prevalence of multidrug-resistance uropathogenic Escherichia coli strains, Isfahan, Iran. Journal of Natural Ccience, Biology, and Medicine, 7: 22.
Díez Villaseñor, C, Almendros, C, García Martínez, J & Mojica, FJ 2010, Diversity of CRISPR loci in Escherichia coli. Microbiology, 156: 1351-1361.
Dyda, F & Hickman, AB 2015, Mechanism of spacer integration links the CRISPR/Cas system to transposition as a form of mobile DNA. Mobile DNA, 6: 1-5.
Foxman, B, Barlow, R, D'arcy, H, Gillespie, B & Sobel, JD 2000, Urinary tract infection: Self-reported incidence and associated costs. Annals of Epidemiology, 10: 509-515.
Garneau, JE, Dupuis, MÈ, Villion, M, Romero, DA, Barrangou, R, Boyaval, P, Fremaux, C, Horvath, P, Magadán, A H & Moineau, S 2010, The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA. Nature, 468: 67-71.
Haft, DH, Selengut, J, Mongodin, EF & Nelson, KE 2005, A guild of 45 CRISPR-associated (Cas) protein families and multiple CRISPR/Cas subtypes exist in prokaryotic genomes. PLoS Computation Biology, 1: 60.
Hassuna, NA, Khairalla, AS, Farahat, EM, Hammad, AM & Abdel-Fattah, M 2020, Molecular characterization of Extended-spectrum β lactamase-producing E. coli recovered from community-acquired urinary tract infections in Upper Egypt. Scientific Reports, 10: 1-8.
Hooton, TM 2001, Recurrent urinary tract infection in women. International Journal of Antimicrobial Agents, 17: 259-268.
Hsu, PD, Lander, ES & Zhang, F 2014, Development and applications of CRISPR-Cas9 for genome engineering. Cell, 157: 1262-1278.
Ishino, Y, Shinagawa, H, Makino, K, Amemura, M & Nakata, A 1987, Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product. Journal of Bacteriology, 169: 5429-5433.
Issazadeh, K, Naghibi, SN & Khoshkholgh Pahlaviani, MRM 2015, Drug resistance and serotyping of uropathogenic Escherichia coli among Patients with urinary tract infection in Rasht, Iran. Zahedan Journal of Research Medical Sciences, 17: 1-5 (In Persian).
Jena, J, Sahoo, RK, Debata, NK & Subudhi, E 2017, Prevalence of TEM, SHV, and CTX-M genes of extended-spectrum β-lactamase-producing Escherichia coli strains isolated from urinary tract infections in adults. 3 Biotech, 7: 244.
Kashef Nejad, M, Jazani, NH & Sharifi, Y 2017, Urinary tract infections among kidney transplant patients due to extended-spectrum beta-lactamase-producing bacteria. Microbial Pathogenesis, 107: 276-279.
Khezri, M, Rezaei, M, Mohabbati Mobarez, A, Zolfaghari, M 2021, The change in heat inactivation of Escherichia coli O157:H7 after entering into the viable but non-culturable state in salted fish, Hypophthalmichthys molitrix. Caspian Journal of Environmental Sciences, 19: 629-637
Liu, H, Wang, Y, Wang, G, Xing, Q, Shao, L, Dong, X, Sai, L, Liu, Y & Ma, L 2015, The prevalence of Escherichia coli strains with extended spectrum beta-lactamases isolated in China. Frontiers in Microbiology, 6: 335.
Livermore, DM & Brown, DF 2001, Detection of β-lactamase-mediated resistance. Journal of Antimicrobial Chemotherapy, 48: 59-64.
Makarova, KS, Haft, DH, Barrangou, R, Brouns, SJ, Charpentier, E, Horvath, P, Moineau, S, Mojica, FJ, Wolf, YI & Yakunin, AF 2011, Evolution and classification of the CRISPR–Cas systems. Nature Reviews Microbiology, 9: 467-477.
Makarova, KS, Wolf, YI, Iranzo, J, Shmakov, SA, Alkhnbashi, OS, Brouns, SJ, Charpentier, E, Cheng, D, Haft, DH & Horvath, P 2019, Evolutionary classification of CRISPR–Cas systems: A burst of class 2 and derived variants. Nature Reviews Microbiology, 1-17.
Merikhi, N, Nowroozi, J, Nazemi, A, Hashemi, M & Tabatabaei, RR 2022, Correlation Among CRISPR-Cas Systems And Antibiotic Resistance blactx-M Gene In UPEC. Journal of Positive School Psychology, 9963-9970.
Ojdana, D, Sacha, P, Wieczorek, P, Czaban, S, Michalska, A, Jaworowska, J, Jurczak, A, Poniatowski, B & Tryniszewska, E 2014, The Occurrence of CTX-M, SHV, and TEM Genes in Extended-Spectrum β-Lactamase-Positive Strains of Klebsiella pneumoniae, Escherichia coli, and Proteus mirabilis in Poland. International Journal of Antibiotics, 14: 935842.
Palmer, KL & Gilmore, MS 2010, Multidrug-resistant enterococci lack CRISPR-cas. mBio, 1.
Palmer, KL & Whiteley, M 2011, DMS3-42: the secret to CRISPR-dependent biofilm inhibition in Pseudomonas aeruginosa. Journal of Bacteriology, 193: 3431-3432.
Pougach, K, Semenova, E, Bogdanova, E, Datsenko, KA, Djordjevic, M, Wanner, BL & Severinov, K 2010, Transcription, processing and function of CRISPR cassettes in Escherichia coli. Molecular Microbiology, 77: 1367-1379.
Pourzare, M, Derakhshan, S & Roshani, D 2017, Distribution of uropathogenic virulence genes in Escherichia coli isolated from children with urinary tract infection in Sanandaj, Iran. Archive of Pediatrics Infectious Disease, 5: 41995.
Samson, JE, Magadan, AH & Moineau, S 2015, The CRISPR‐Cas immune system and genetic transfers: Reaching an equilibrium. Plasmids: Biology and Impact in Biotechnology and Discovery, 209-218.
Tabasi, M, Karam, MRA, Habibi, M, Mostafavi, E & Bouzari, S 2016, Genotypic characterization of virulence factors in Escherichia coli isolated from patients with acute cystitis, pyelonephritis and asymptomatic bacteriuria. Journal of Clinical and Diagnostic Research, 10: DC01.
Tonekabony, N, Izadi Amoli, R, Gholami, A & Oskoueiyan, R 2021, Prevalence of SHV gene and antibiotic resistance of extended-spectrum β-lactamases-producing Escherichia coli strains isolated from abattoir wastewater in Mazandaran, North of Iran. Caspian Journal of Environmental Sciences, 19: 11 - 17
Touchon, M, Charpentier, S, Clermont, O, Rocha, EP, Denamur, E & Branger, C 2011, CRISPR distribution within the Escherichia coli species is not suggestive of immunity-associated diversifying selection. Journal of bacteriology, 193: 2460-2467.
Touchon, M, Charpentier, S, Pognard, D, Picard, B, Arlet, G, Rocha, EP, Denamur, E & Branger, C 2012, Antibiotic resistance plasmids spread among natural isolates of Escherichia coli in spite of CRISPR elements. Microbiology, 158: 2997-3004.
Wang, G, Song, G & Xu, Y 2020, Association of CRISPR/Cas System with the Drug Resistance in Klebsiella pneumoniae. Infection and Drug Resistance, 13, 1929.
Westra, ER, Pul, Ü, Heidrich, N, Jore, MM, Lundgren, M, Stratmann, T, Wurm, R, Raine, A, Mescher, M & Van Heereveld, L 2010, H‐NS‐mediated repression of CRISPR‐based immunity in Escherichia coli K12 can be relieved by the transcription activator LeuO. Molecular Microbiology, 77: 1380-1393.
Xiong, Y, Fowler Jr, V, Yeaman, M & Perdreau Remington, F 2019, Watts, A, Ke, D, Wang, Q, Pillay, A, Nicholson-Weller, A & Lee, JC 2005, Staphylococcus aureus strains that express serotype 5 or serotype 8 capsular polysaccharides differ in virulence. Infection and immunity, 73, 3502-3511. WAYNE, P 2014, Clinical and Laboratory Standards Institute: Performance standards for antimicrobial susceptibility testing: Twenty-fourth informational. Departament De Microbiología I Ecología, 25: 167.
Yazdi, M, Nazemi, A, Mirinargasi, M, Jafarpour, M, Sharifi, S & Branch, T 2012, Genotypic versus Phenotypic methods to detect extended-spectrum beta-lactamases (ESBLs) in uropathogenic Escherichia coli. Annals of Biological Research, 3: 2454-8.
Yousif, M, Radhi, S, H, Auda,S, A 2022, Evaluation of tap water quality and molecular identification of Escherichia coli O157 in Al-Kut City, Iraq. Caspian Journal of Environmental Sciences, 20: 1053-1057.
Zhao, R, Shi, J, Shen, Y, Li, Y, Han, Q, Zhang, X, Gu, G & Xu, J 2015, Phylogenetic distribution of virulence genes among ESBL-producing uropathogenic Escherichia coli isolated from long-term hospitalized patients. Journal of Clinical and Diagnostic Research, 9: DC0. | ||
|
آمار تعداد مشاهده مقاله: 212 تعداد دریافت فایل اصل مقاله: 151 |
||