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شناسایی QTLهای مرتبط با مولفههای جوانهزنی جو (Hordeum vulgare L) در شرایط نرمال، تنش خشکی و شوری | ||
علوم و تحقیقات بذر ایران | ||
مقاله 6، دوره 9، شماره 1، فروردین 1401، صفحه 51-66 اصل مقاله (1.53 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22124/jms.2022.6145 | ||
نویسندگان | ||
سمیه مختوم1؛ حسین صبوری* 2؛ عبداللطیف قلیزاده3؛ لیلا آهنگر3؛ مهناز کاتوزی4 | ||
1دانشجوی ارشد بیوتکنولوژی، گروه تولیدات گیاهی، دانشکده علوم کشاورزی و منابع طبیعی، دانشگاه گنبد کاووس، ایران | ||
2دانشیار گروه تولیدات گیاهی، دانشکده علوم کشاورزی و منابع طبیعی، دانشگاه گنبد کاووس، ایران | ||
3استادیار گروه تولیدات گیاهی، دانشکده علوم کشاورزی و منابع طبیعی، دانشگاه گنبد کاووس، ایران | ||
4گروه اصلاح نباتات و منابع ژنتیکی، اگروسکوپ، سوئیس | ||
چکیده | ||
جوانهزنی بذر از خصوصیات بارز و مهم یک رقم زراعی توصیف میشود. بهمنظور شناساییQTLهای مرتبط با جوانهزنی جو تحت شرایط نرمال، شوری و خشکی، 103 خانواده F8 حاصل از تلاقی دو رقم بادیا×کویر در قالب طرح کاملا تصادفی در سال 1398-1399 در آزمایشگاه گیاهشناسی دانشگاه گنبدکاووس در دو تکرار بررسی شدند. تعداد ریشهچه، طول ریشهچه، وزن ریشهچه، طول کلئوپتیل، طول ساقهچه، وزن ساقهچه و درصد جوانهزنی مورد اندازهگیری قرار گرفتند. نقشه پیوستگی بر اساس 152 آلل چند شکل SSR، 72 آللISSR ، 7 آلل IRAP، 29 آللCAAT ، 27 آلل Scot و 15 آلل iPBS تهیه شد. نشانگرهای مولکولی استفاده شده هفت کروموزوم جو با طول نقشه 2/999 سانتیمورگان منتسب شد. میانگین فاصله بین دو نشانگر مجاور برابر 387/3 سانتیمورگان شد. تجزیه QTL بهروش نقشهیابی فاصلهای مرکب (CIM) برای هر صفت در هر محیط انجام شد. در این پژوهش در هر سه شرایط کروموزومهای 4، 5 و 7 بهدلیل قرارگرفتن بیشترینQTLها بهعنوان مهمترین کروموزومها بودند. در شرایط نرمال دوQTL بزرگ اثر برای طول کلئوپتیل (qCLN-5a) و درصد جوانهزنی (qGPN-4b) ردیابی شد، در شرایط تنش خشکی سهQTL بزرگ اثر برای طول ریشه (qRLD-4b) و (qRLD-5) برای وزن ساقهچه (qPWD-4) ردیابی شد. از نتایج این پژوهش بعد از تعیین اعتبار میتوان در برنامههای انتخاب به کمک نشانگر استفاده نمود. | ||
کلیدواژهها | ||
انتخاب به کمک نشانگر؛ تنش شوری و خشکی؛ جو؛ جوانهزنی؛ نشانگرهای مولکولی | ||
مراجع | ||
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