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بررسی سیستم آنتیاکسیدانت غیرآنزیمی، کمیت پروتئین و روغن در شرایط پیری تسریعشده بذرهای دو رقم کلزا (Brassica napus L.) | ||
علوم و تحقیقات بذر ایران | ||
مقاله 2، دوره 9، شماره 1، فروردین 1401، صفحه 1-14 اصل مقاله (2.21 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22124/jms.2022.6141 | ||
نویسندگان | ||
مریم زمانیان1؛ رضا توکلافشاری* 2؛ جعفر نباتی3؛ علیرضا سیفی4 | ||
1دانشآموخته کارشناسیارشد علوم و تکنولوژی بذر، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران | ||
2استاد گروه اگروتکنولوژی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران | ||
3استادیار پژوهشکده علوم گیاهی، دانشگاه فردوسی مشهد، مشهد، ایران | ||
4استادیار گروه بیوتکنولوژی وبه نژادی گیاهی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران | ||
چکیده | ||
این پژوهش با هدف بررسی اثر پیری تسریعشده (AA) بر شاخصهای فیزیولوژیک و بیوشیمیایی بذر کلزا در قالب آزمایش فاکتوریل بر مبنای طرح کاملاً تصادفی با دو عامل رقم (مودنا و اوکاپی)، سطح پیری (صفر، 24 و 72 ساعت دمای 40 درجه سلسیوس و رطوبت اشباع) و چهار تکرار در سال 99-1398 در دانشگاه فردوسی مشهد انجام شد. نتایج نشان داد که بذرهای پیر شده ارقام اوکاپی و مودنا، کاهش معنیداری در شاخصهای جوانهزنی داشتند. در تیمار 72 ساعت پیری در مقایسه با شاهد، محتوای روغن، پروتئین و فعالیت مهار آنتیاکسیدانت، بهترتیب، 89/9، 14/4 و 61/28 درصد کاهش یافت. اما نشت الکترولیتها 64/84 درصد نسبت به شاهد افزایش یافت. پیری، محتوای آنتیاکسیدانتهای غیر آنزیمی را نیز تحت تأثیر قرار داد. در رقم اوکاپی، فلاونوئیدها 79/73 درصد، پرولین 15/47 درصد و فنل 13/17 درصد در مقایسه با بذرهای پیر نشده کاهش یافت. رقم اوکاپی، هر چند دارای خصوصیات کیفی مانند درصد روغن، فعالیت سیستمهای آنتیاکسیدانت بالاتری بود، اما به انبارداری بسیار حساس بود، چرا که پیری باعث افت شدید کیفیت بذرهای رقم اوکاپی شد. بنابراین برای جلوگیری از خسارتهای اقتصادی، باید به شرایط محیطی نگهداری بذرهای این رقم، توجه بیشتری گردد. بهطورکلی، کنترل شرایط انبارداری برای به حداقل رساندن پیری و زوال بذر کلزا، امری حیاتی است. | ||
کلیدواژهها | ||
اوکاپی؛ پرولین؛ فلاونوئید؛ فنل؛ مودنا | ||
مراجع | ||
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