|تعداد مشاهده مقاله||7,633,710|
|تعداد دریافت فایل اصل مقاله||5,854,632|
Evaluation of tap water quality and molecular identification of Escherichia coli O157 in Al-Kut City, Iraq
|Caspian Journal of Environmental Sciences|
|دوره 20، شماره 5، اسفند 2022، صفحه 1053-1057 اصل مقاله (724.29 K)|
|نوع مقاله: Research Paper|
|شناسه دیجیتال (DOI): 10.22124/cjes.2022.6079|
|Malath I. Yousif1؛ Safa Hasan Radhi* 2؛ Shaimaa Awadh Auda3|
|1Al-Furat Al-Awsat technical university, Technical Institute, Diwanya , Iraq|
|2Department of Biology, College of Science, Al-Qasim Green University, Iraq|
|3College of dentistry, Al-Muthanna University, Iraq|
|Escherichia coli is a pathogen which can result in gastrointestinal infection. It can be transferred in a many methods, like water and food. This study is aimed to evaluate the potable water quality in Al- Kut City, Iraq, in addition to diagnosis of E. coli O157:H7 by PCR protocol. The method includes examination for total dissolved solids (TDSs), pH and electrical conductivity (EC), as well as the bacterial DNA extraction by DNA extraction kit, then detection via PCR. Primer "Rfb" was used for detection of O157 gene. The bands of PCR product was 292 bp, which has been visualized using gel electrophoresis. The results gained revealed that the advanced procedure by molecular detection may be used as a routine examination for drinking water pollution. Moreover, it is a precise procedure providing results at a less time compared to the classical culture methods.|
|PCR؛ Rfb؛ E.coli؛ TDS؛ pH؛ EC|
Ahmad, M, Bajahlan, AS 2009, Quality comparison of tap water vs. bottled water in the industrial city of Yanbu (Saudi Arabia). Environmental Monitoring and Assessment, 159: 1-14.
AL Dulaimi, GA & Younes, MK 2017, Assessment of Potable Water Quality in Baghdad City, Iraq. Air, Soil and Water Research, 10: 15.
Bain, R, Cronk, R, Wright, J, Yang, H, Slaymaker, T & Bartram, J 2014, Faecal contamination of drinking-water in low-and middle-income countries: a systematic review and meta-analysis. PLOS Medicine, 2014: 11:e1001644.
Batarseh, MI 2006, The quality of potable water types in Jordan. Environmental Monitoring and Assessment, 117: 235-244.
Bonetta, S, Borelli, E, Conio, O, Palumbo, F & Carraro, E 2011, Development of a PCR protocol for the detection of Escherichia coli O157:H7 and Salmonella spp. in surface water. Environmental Monitoring and Assessment, 177: 493-503.
Bruvold, WH & Ongerth, HJ 1969, Taste quality of mineralized water. Journal of the American Water Works Association, 61: 170.
Edberg, S, Rice, EW & Allen, MJ 2000, Escherichia Coli: The Best Biological Drinking Water Indicator for Public Health Protection. Journal of Applied Microbiology, 88: 1068-1168.
Farabi, SMV, Golaghaei, M, Sharifian, M, Daryanabard, G, Karimian, E 2022, Effects of rainbow trout farming on water quality around the sea farms in the south of the Caspian Sea. Caspian Journal of Environmental Sciences, 20: 729-737.
Fatih Ali, S, Hamud Hays, H, Abdul-Jabar, R 2021, Application of CCME water quality index for drinking purpose in Tigris River within Wasit Province, Iraq. Caspian Journal of Environmental Sciences, 19: 781-787.
Faysal, M & Juliana, FM 2017, Assessment of pH and total dissolved substances (TDS) in the commercially available bottled drinking water. IOSR Journal of Nursing and Health Science, 6: 35-40.
Gray, NF 2008, Drinking water quality: Problems and solutions. Cambridge, UK: Cambridge University Press.
Greenberg AE, Clesceri, LS 1992, Standard methods for the examination of water and wastewater. washington, dc: American Public Health Association.
Griffin, PM 1995, Escherichia coli O157:H7 and other entero-haemorrhagic Escherichia coli. In Infections of Gastrointestinal Tract. MJ, Blaser, PD, Smith, JI, Ravdin, HB Greenberg & RL Guerrant (Eds.), New York, Raven Press, pp. 739-761.
Gunzer, F, Bohm, H, Russmann, H, Bitzan, M, Aleksic, S & Karchl, H 1992, Molecular detection of Sorbitol-Fermenting Escherichia coli 0157 in Patients with Hemolytic-Uremic Syndrome. Journal of Clinical Microbiology, 30: 1807-1810.
Hadi Dehghani, M, Khaniki, GRJ, Mohammadi, H et al 2011, Microbiological quality of drinking water in Shadegan Township, Iran. Iranian Journal of Energy and Environment, 2: 286-290 (In Persian).
Hendrickson, K 2017, Drinking water pH levels. Retrieved from http://www.thedai.lystar.ne t/health/what-s ho uld-be-the-ph-value-dri nking-water-138382
Heidari, AR, Mortazavi, S & Hasanzadeh, N 2022, Spatiotemporal variation analysis of water quality using multivariate statistical methods, Case study: Koohsar Lake, Western Iran. Caspian Journal of Environmental Sciences, 20: 711-720.
Imtiaz, J, Hashmi, I, Saeed, A, Qazi, IA & Arshad, M 2013, Development of PCR protocol for detection of Escherichia coli in drinking water. WIT Transactions on Ecology and the Environment, 171: 225-234.
Innis, AM, Gelfand, HD, Sninsky, JJ & White, JT 1990, Optimization of PCRs. Chapter 1, PCR protocols, A guide to methods and applications, Academic Press, INC: New York, pp. 3-11.
Islam, R, Faysal, SM, Amin, MR, Juliana, FM, Islam, MJ, Alam, MJ, Hossain, MN & Asaduzzaman, M 2017, Assessment of pH and total dissolved substances (TDS) in the commercially available bottled drinking water. IOSR Journal of Nursing and Health Science, 6: 35-40.
Meng, J, Zhao, S, Doyle, MP, Mitchell1, SE & Kresovich, IS 1997, A multiplex PCR for identifying Shiga-like toxin-producing Escherichia coli O157:H7. Letters in Applied Microbiology, 24: 172-176.
Omidi, A, Shariati, F 2021, Evaluation of Pasikhan River, North of Iran Water Quality Using Water Quality Index (NSFWQI), Caspian Journal of Environmental Sciences, 19: 219-230.
Parma, AE, Vinas, MR & Sanz, ME 1996, Improvement of the polymerase chain reaction to detect Escherichia coli Shiga like toxin II gene from clinical isolates. Journal of Microbiological Methods, 26: 81-85.
Sasikaran, S, Sritharan, K, Balakumar, S, Arasaratnam, V 2012, Physical, chemical and microbial analysis of bottled drinking water. Ceylon Medical Journal, 57: 111116.
SDWF. Retrieved from https://www.safewater.org/fact-sheets-1/2017/1/23/tds-and-ph.
Tsen, HY & Jian, LZ 1998, Development and use of a multiplex PCR system for the rapid screening of heat liable toxin I, heat stable toxin II and Shiga-like toxin I and II genes of Escherichia coli in water. Journal of Applied Microbiology, 84: 585592.
USFDA 2017, Retrieved from https://www. Accessdata .fda.gov/scripts/cdrh /cfdocs/cfcfr /CFRSearch .cfm? fr=165.110.
USGS 2016, Retrieved from https://water.usgs.gov/edu/ph.html.
WHO 1996, Guidelines for drinking-water quality. Vol. 2, Health criteria and other supporting information. World Health Organization, Geneva.
WHO 2004, WHO Guidelines for Drinking-Water Quality: Recommendations. Vol. 1, World Health Organization, Geneva, Switzerland.
تعداد مشاهده مقاله: 120
تعداد دریافت فایل اصل مقاله: 132