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مقایسه الگوی بیان ژن در بافت روپوش صدف مرواریدساز پارسی Pinctada persicaدر دو فصل گرم و سرد | ||
فیزیولوژی و بیوتکنولوژی آبزیان | ||
دوره 10، شماره 2، مهر 1401، صفحه 121-139 اصل مقاله (1.03 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22124/japb.2021.20297.1431 | ||
نویسندگان | ||
فاطمه پرویزی1؛ ابوالفضل ناجی2؛ آرش اکبرزاده2؛ احمد فرهادی3؛ محمدشریف رنجبر* 4، 5 | ||
1دکتری تکثیر و پرورش آبزیان، دانشکده علوم و فنون دریایی، دانشگاه هرمزگان، بندرعباس، ایران | ||
2دانشیار گروه شیلات، دانشکده علوم و فنون دریایی، دانشگاه هرمزگان، بندرعباس، ایران | ||
3استادیار گروه مهندسی منابع طبیعی و محیط زیست، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ایران | ||
4دانشیار گروه زیستشناسی دریا، دانشکده علوم و فنون دریایی، دانشگاه هرمزگان، بندرعباس، ایران. | ||
5دانشیار پارک زیستفناوری خلیج فارس، جزیره قشم، ایران | ||
چکیده | ||
با توجه به اثر قابل توجه دما بر فرآیند معدنی سازی زیستی، بررسی تاثیر تغییرات فصلی بر عملکرد و الگوی بیان ژن بافت روپوش، عضو مسئول معدنی سازی زیستی در صدف های مرواریدساز، ضروری است. در این مطالعه، الگوی بیان 9 ژن معدنی ساز زیستی (ASP، KRMP، SHEM5، PRISM، PEARL، CHIT، PIF، MRNP34 و NACREIN) در بافت روپوش صدف مرواریدساز Pinctada persica در دو فصل گرم و سرد مورد بررسی قرار گرفت. cDNA از بافت روپوش، با استفاده از کیت حاوی آنزیم رونویسی معکوس ساخته شد و میزان بیان ژن به روش qPCR مورد سنجش قرار گرفت. نتایج نشاندهنده الگوی بیان ژنی متفاوت بین دو فصل گرم و سرد بود. سه ژن مربوط به لایه پریسماتیک (ASP، KRMP و SHEM5) در فصل سرد و یک ژن مربوط به لایه نقیر (PIF) در فصل گرم، بیان بیشتری را نشان دادند. به نظر می رسد نرخ فعالیت معدنی سازی زیستی در دمای بالاتر کاهش یافته و ژنهای لایه پریسماتیک نقش مهمتری در واکنش به تغییرات فصلی داشته باشند. این مطالعه، اطلاعات ارزشمندی را برای شناخت بهتر بافت روپوش گونه P. persica و تاثیر عامل محیطی بر مکانیسم های مولکولی موثر در شکلگیری پوسته و مروارید فراهم کرده است. | ||
کلیدواژهها | ||
ژنهای معدنیساز زیستی؛ بافت روپوش؛ Pinctada persica؛ فصل گرم؛ فصل سرد | ||
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