|تعداد مشاهده مقاله||7,632,894|
|تعداد دریافت فایل اصل مقاله||5,853,966|
Screening for bioactive secondary metabolites in Actinomycetes isolated from soils around old buckthorn and palm trees
|Caspian Journal of Environmental Sciences|
|دوره 20، شماره 3، مهر 2022، صفحه 519-526 اصل مقاله (1.13 M)|
|نوع مقاله: Research Paper|
|شناسه دیجیتال (DOI): 10.22124/cjes.2022.5683|
|Talib Saleh A-Rubaye1؛ Dalal Al-Rubaye* 1؛ Marwa Shaker1؛ Mohsen Hashim Risan2|
|1Department of Biotechnology, College of Science, University of Baghdad, Baghdad, Iraq|
|2Biotechnology College, AL-Nahrain University, Iraq|
|Antibiotic resistance is one of the biggest public health threats increased globally in this century. This study is aimed to determine the compounds with antimicrobial activity exhibited in Actinobacteria as a source for new antibiotic to treat highly-resistant bacteria. The Actinobacteria isolated from the soil around buckthorn and palm old trees using IPS4 agar media for first screening. ISP2 agar and broth media were used for Actinobacteria identification and fermentation and secondary metabolites production respectively. Secondary metabolites screening was done using extracellular crude extract against extended spectrum beta-lactamases (ESBLs) producing Escherichia coli, Klebsiella pneumonia and Pseudomonas aeruginosa and beta lactam resistant Staphylococcus aureus by well diffusion method. The total number of Actinobacterial isolates was 10, most of them showed good antimicrobial activity appeared by a particularly good zone of inhibition around resistant bacteria. This finding gives a promise that Streptomyces in soil around buckthorn and palm old trees considered as a source for new antibiotics.|
|Streptomyces؛ ESBLs؛ Multidrug resistance؛ Bioactive compounds|
Abdullah, SK, Al Samarraie, MQ & Al Assie, AH 2015, Fungi associated with grapevine (Vitis vinifera L) decline in middle of Iraq. Egyptian Academic Journal of Biological Sciences, G. Microbiology, 7: 53-59.
Al Rubaye, D, Talib, T & Marwa, S 2020, Antibacterial activities of river sediment-derived Streptomyces spp. against multi drug resistant Klebsiella pneumoniae. RJPBCS, 11: 95.
Al Rubaye, DS 2016, Phylogenetic analysis of streptomyces spp. exhibited different antimicrobial activities. Iranian Journal of Science and Technology, 57: 397-403.
Al Rubaye, TS, Mohsen, HR, Al Rubaye, D, Rabab, OR 2018, Identification and in vitro antimicrobial activity of marine Streptomyces spp. bacteria from Tigris River sediments in Baghdad City. World Journal of Plastic Surgery, 4: 120-134.
AL Samarraie, MQ & Al Assie, AH 2014, New records of some saprophytic and pathogenic fungi isolated from declining grapevine in Salahaldin Province, middle Iraq. Tikrit Journal of Pure Science, 19.
AL Samarraie, MQ, Omar, MK, Yaseen, AH & Mahmood, MI 2019, The wide spread of the gene haeomolysin (Hly) and the adhesion factor (Sfa) in the E. coli isolated from UTI. Journal of Pharmaceutical Sciences and Research, 11: 1298-1303.
Aly, MM, Samyah, J & Mohammed, GK 2013, Antagonistic properties of Streptomyces isolated from forest soils against fungal pathogens of pine seedlings. International Journal of Pharma and Bio Sciences, 4: 12-21.
Arifuzzaman, M, Khatun, MR & Rahman, H 2010, Isolation and screening of actinomycetes from Sundarbans soil for antibacterial activity, African Journal of Biotechnology, 9: 4615-4619.
Buchanan, RE & Gibbns, NE 1974, Bergey’s manual of determinative bacteriology (8th ed.). The Williams and Wilkins Co., Baltimore. https://doi.org/10.1111/j.1550- 7408.1975. tb00935.x.
Campos, MA, Vargas, MA, Regueiro, V, Llompart, CM, Albertí, S, Bengoechea, JA 2004, Capsule polysaccharide mediates bacterial resistance to antimicrobial peptides. Infection and Immunity. 72: 7107-7114.
Dehbandi, N, Izadi Amoli, R, Oskoueiyan, R, Gholami, A 2019, The prevalence of vanA gene in clinical isolates of vancomycin-resistant Staphylococcus aureus in a hospital in Mazandaran, Iran. Caspian Journal of Environmental Sciences, 17: 319-325
Drawz, SM & Bonomo, RA 2010, Three decades of beta-lactamase inhibitors. Clinical Microbiology Reviews.23: 160-201.
Franco Correa, M, Quintana, A, Duque, C, Suarez, C, Rodríguez, MX & Barea, JM 2010, Evaluation of actinomycete strains for key traits related with plant growth promotion and mycorrhiza helping activities. Applied Soil Ecology, 5: 209-217.
Genilloud, O 2017, Actinomycetes: still a source of novel antibiotics. Natural Product Reports. 34: 1203-1232.
Heydari, S & Eftekhar, F 2015, Biofilm formation and β-lactamase production in burn isolates of Pseudomonas aeruginosa. Jundishapur Journal of Microbiology, 8: e15514.
Jog, R, Nareshkumar, G & Rajkumar, S 2016, Enhancing soil health and plant growth promotion by actinomycetes. Springer, pp. 33-45.
Khan, JA & Patel, AS 2011, Extraction and purification of antibacterial metabolites from actinomycetes spp. isolated from soil sample. Journal of Pharmaceutical Research and Development. 3: 63-71.
Korn Wendisch, F & Kutzner, HJ 1992, The family Streptomycetaceae. In: A, Balows, HG, Truper, M, Dworkin, W, Harder & KH, Schleifer, (eds.), The prokaryotes. Springer-Verlag, New York, 921-995.
Kumari, KS, Kalyani, P, Vineela, KC, Hemalatha, V & Hemalatha, KPJ 2017, Isolation of antibiotic producing actinomycetes from untapped soils of Yarada hills and assessment of their antimicrobial activities. International Journal of Scientific Research in Science, Engineering and Technology, 3: 290–293.
Livermore, D 2002, Detection of β-lactamase mediated resistance, p. 1-18. In: Standardized disc susceptibility method. User Day Report for November 2002 Meeting Held at Belfast City Hospital, Belfast, British Society for Antimicrobial Chemotherapy, Birmingham, United Kingdom.
National Committee for Clinical Laboratory Standards Laboratory 2010, Detection of Extended-Spectrum β-Lactamases (ESBLs). NCCLS approved standard M100-S9. National Committee for Clinical Laboratory Standards, Wayne, PA; 2010.
Olanrewaju, OS & Babalola, OO 2019, Streptomyces: implications and interactions in plant growth promotion. Applied Microbiology and Biotechnology, 103: 1179-1188.
Pallavi, S, Manasa, M, Kambar, Y, Asha, M, Chaithra, M, Vivek, M & Mallikarjun, N 2013, Anti-Staphylococcus aureus and Anti-yeast activity of Streptomyces species isolated from rhizosphere soil of Sahyadri Science College, Shivamogga, Karnataka. Asian Journal of Biomedical and Pharmaceutical Sciences. 3: 7-11.
Pérez Moreno, MO, Katargina, O, Pérez Moreno, M, Carulla, M, Rubio, C, Jardí, AM & Zaragoza, J 2004, Mechanisms of reduced susceptibility to amoxycillin-clavulanic acid in Escherichia coli strains from the health region of Tortosa (Catalonia, Spain), Clinical Microbiology and Infection, 10: 234-24.
Sharafkhah, S, Oskoueiyan, R, Izadi Amoli, R, Gholami, A 2022, Extended-spectrum beta-lactamases detection and prevalence of blaTEM gene in clinical isolates of Klebsiella pneumoniae from hospitals in North of Iran. Caspian Journal of Environmental Sciences, 20: 29-35.
Teklu, DS, Negeri, AA, Legese, MH, Bedada, TL, Woldemariam, HK & Tullu, KD 2019, Extended-spectrum beta-lactamase production and multi-drug resistance among Enterobacteriaceae isolated in Addis Ababa, Ethiopia. Antimicrobial Resistance and Infection Control, 15; 8:39.
Tonekabony, N, Izadi Amoli, R, Gholami, A, Oskoueiyan, R 2021, Prevalence of SHV gene and antibiotic resistance of extended-spectrum β-lactamases-producing Escherichia coli strains isolated from abattoir wastewater in Mazandaran, North of Iran. Caspian Journal of Environmental Sciences, 19: 11-17
Ugwuanyi, FC, Ajayi, A, Ojo, DA, Adeyemi, IA, Stella, IS 2021, Evaluation of efflux pump activity and biofilm formation in multidrug resistant clinical isolates of Pseudomonas aeruginosa isolated from a Federal Medical Centre in Nigeria. Annals of Clinical Microbiology and Antimicrobials, 20: 11.
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