|تعداد مشاهده مقاله||7,639,941|
|تعداد دریافت فایل اصل مقاله||5,858,506|
Antibacterial Activity of Biosurfactant extracted from Streptococcus thermophilus and Its effect on some Biochemical Parameters in Male Rats
|Caspian Journal of Environmental Sciences|
|دوره 20، شماره 1، فروردین 2022، صفحه 131-136 اصل مقاله (670.95 K)|
|نوع مقاله: Research Paper|
|شناسه دیجیتال (DOI): 10.22124/cjes.2022.5407|
|Batool Shakir Abed Almjalawi* 1؛ Thoalffakar A. Alhamed2؛ Ahmed Abdulkareem Almutteari1|
|1General Directorate of Education in Karbala, Karbala, Iraq|
|2University of Warith AL-Anbiyaa, College of Nursing, Karbala, Iraq|
Streptococcus Thermophilus belongs to the group of lactic acid bacteria. The current study aims to identify the role of the biosurfactant produced by S. thermophilus bacteria, showing its importance in limiting the growth of pathogens and its effect on the biochemical parameters of male rats. S. thermophilus bacteria were isolated from local cheese, 120 different samples were collected from the human body (urine, sputum, wounds), and the biosurfactant was extracted from the bacteria. the active groups of biosurfactant components were detected and the antibacterial activity was tested on some biochemical parameters in male rats. 42 infected samples were distributed between 16 for urine, 11 for sputum and 15 for wound samples. The results showed that the highest percentage of infection with Staphylococcus aureus was 12 (28.57%), while the lowest Staphylococcus epidermidis was 3 (7.14%). The qualitative tests showed that the biosurfactant contains many active components such as tannins, carbohydrates, phenols, flavonoids, and sapindales, while does not contain triterpenoids. The results of the statistical analyses to anti-bacterial activity showed a significant increase (p<0.05), which indicates its effect on some biochemical parameters in male rats. A significant decrease was found in the total cholesterol, triglycerides and high-density lipoprotein (LDL) concentrations in the treatment group in comparison with the control. It was also noted a significant increase in HDL compared to control group. We conclude that biosurfactant has the ability to eliminate some gram-positive and gram-negative bacteria isolated from different clinical infections of the human body and it has an effective effect on the biochemical parameters of the male rats.
|Biosurfactant؛ Streptococcus thermophilus؛ Biochemical parameters؛ Rats|
Adedayo, O, Anderson, W, Young, M, Sncickus, V, Patil, P & Kolawole, D 2001, Phytochemistry and antibacterial activity of Senna alata flower. Pharmaceutical Biology, 39: 1-5.
Ahmed, M, Nazil, S, & Anwar, M 1989, Studies on tannins from bark of Pinus roxburghii. Journal of the Chemical Society of Pakistan, 11: 213-217.
Akpinar, A, Yerlikaya, O & Kiliç, S 2011, Antimicrobial activity and antibiotic resistance of Lactobacillus delbrueckii ssp. Bulgaricus and Streptococcus thermophilus strains isolated from Turkish homemade yoghurts. African Journal of Microbiol Research.
Allain, CC, Poon, LS, Chan, CSG & Richmond, WFC 1974, The Merk manual of diagnostic and therapy, Merk Co. Clinical Chemistry, 20: 470-475.
Al-khazraji, SM 1991, Biopharmacological study of Artemisia herba-lba. MSc. Dissertation, College of Pharmacy. University of Baghdad.
Aslam, M, Shahid, M, Rehman, FU, Naveed, NH, Batool, AI, Sharif, S & Asia, A 2011, Purification and characterization of bacteriocin isolated from Streptococcus thermophilus. African Journal of Microbiology Research, 5: 2642-2648.
Burstein, MJ 1970, Measurement of HDL. Journal of Lipid Research, 11: 583.
Chotkowska, E, Kurjata, P & Kupsc, W 2001, Evaluation of the precision of the friedewaldsnsformula for the calculuation of LDL-C concentraction in serum. Polski Merkuriusz Lekarski, 11: 348-351.
Delcour, J, Ferain, T & Hols, P 1996, Advances in the genetics of thermophilic lactic acid bacteria. Current Opinion in Biotechnology, 70: 497-504.
FAO & WHO 2002, Guidelines for the evaluation of probiotics in food drafting guidelines for the evaluation of probiotics in food London Ontario, Canada.
Forbes, BA, Saham, DF & Weissfeld, AS 2002, Diagnostic microbiology. 10th ed. Mosby. Inc. U.S.A.
Friedewald, W, Levy, R & Fredirckson, D 1972, Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clinical Chemistry, 18: 199.
Gupta, U, Radramma Rati, ER & Joseph, R 1998, Nutritional Quality of lactic acid fermented bitter gourd and fenugreek leaves. International Journal of Food Sciences and Nutrition, 94: 101-108.
Harborne, JB 1984, Phytochemical methods. A guide to modern techniques of plants analysis. 2nd. ed. Chapman & Hall, London, New York.
Hardie, JM & Whiley, RA 1995, The Genus Streptococcus. In: The Genera of Lactic Acid Bacteria, edited by Wood, BJ & Holzapfel,WH.
Hassam, AN & Frank, JF 2001, Starter cultures and their use. In: Applied Dairy Microbiology. 2nd ed. Edit by Marth, E, Hand steele, LJ, Marcel Dekker, Inc., USA.
Meyer, E & Wather, A 1988, Methods for the estimation of protein, lipid, carbohydrate and chitin in fresh water invertebrates. Journal of Arch. Hydroboilogy, 13: 161-177.
Nurhajati, J, Chrysanti, I, Indrawati, I & Syaftika, N 2008, Antibacterial activity of L. Bulgaricus and S. Thermophilus soygurt cultures, Proceeding of ASEAN Congress of Tropical Medicine and Parasitology, 3: 51-58.
Parente, E & Cogan, TM 2004, Starter cultures: General aspects. In Cheese, Chemistry, Physics and Microbiology, 3rd Edition ed. Fox, PF, mcsweeney, PLH, Cogan, TM & Guinee, TP, London: Elsevier Academic Press, pp. 123-148.
Petti, S, Tarsitani, G & Simonetti D’Arca, A 2008, Antibacterial activit of yoghurt againts viridans streptococci in vitro. Archives of Oral Biology, 53: 985-990.
Richmond, W 1973, Preparation and properties of cholesterol oxidase from Nocard sp. and its application to the enzymatic assay of total cholesterol. Clinical Chemistry, 19: 1350-1356.
Robinson, RK, Tamime, AY & Wszolek, M 2002, Microbiology of ferment milks. In: Dairy Microgiology Hand book, 3th ed., Edited by Robinson, RK Wiley-Interscience, Inc.
Rodrigues, L, Van Der Mei, H, Banat, IM, Teixeira, J & Oliveira, R 2006, Inhibition of microbial adhesion to silicone rubber treated with biosurfactant from Streptococcus thermophilus. FEMS Immunology & Medical Microbiology, 46: 107-112.
Rodrigues, LR, Jos´e, A, Henny, C, Van der, M & Ros´ario, O 2006, Isolation and partial characterization of a biosurfactant produced by Streptococcus thermophilus. Biointerfaces, 53: 105-112.
Sreekumar, O & Hosono, A 2000, Immediate effect of Lactobacillus acidophilus on the intestinal flora and fecal enzymes of rats and the in vitro inhibition of Escherichia coli in coculture. Journal of Dairy Science, 83: 931-939.
Šušković, J, Kos, B, Beganović, J, Leboš, Pavunc, A, Habjanič, K & Matošić, S 2010, Antimicrobial activity- The most important property of probiotic and starter lactic acid bacteria. Food Technology and Biotechnology, 48: 296-307.
Ukeyima, MT, Enujiugha, VN & Sanni, TA 2010, Current applications of probiotic foods in Africa. African Journal of Biotechnology, 9: 394-401.
Vuyst, LDe & Leroy, F 2010, Bacteriocins from lactic acid bacteria: Production, purification, and food applications. Journal of Molecular Microbiology and Biotechnology, 13:194-199.
Seltman, HJ 2012, Experimental design and analysis, Online at: http://www stat. Cmu. Edu/, hseltman/309/Book/Book. Pdf.
تعداد مشاهده مقاله: 964
تعداد دریافت فایل اصل مقاله: 1,226