|تعداد مشاهده مقاله||7,639,945|
|تعداد دریافت فایل اصل مقاله||5,858,525|
Extended-spectrum beta-lactamases detection and prevalence of blaTEM gene in clinical isolates of Klebsiella pneumoniae from hospitals in North of Iran
|Caspian Journal of Environmental Sciences|
|دوره 20، شماره 1، فروردین 2022، صفحه 29-35 اصل مقاله (678.04 K)|
|نوع مقاله: Research Paper|
|شناسه دیجیتال (DOI): 10.22124/cjes.2022.5389|
|Sharareh Sharafkhah1؛ Roghayeh Oskoueiyan* 1؛ Rabeeh Izadi Amoli1؛ Alamara Gholami2|
|1Department of Biology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran|
|2Department of Biology, Sari Branch, Islamic Azad University, Sari, Iran|
|Klebsiella pneumoniae is a member of the Enterobacteriaceae family which plays an important role in creating various infections in the community. These strains are resistant to multiple β-lactam antibiotics due to the production of β-lactamases enzyme (ESBL). The purpose of this study was to assess the phenotypic and genotypic characteristics of ESBL-producing K. pneumoniae strains from clinical specimens in three hospitals in Mazandaran, Iran. In this cross-sectional study, K. pneumoniae samples (N = 100) were identified from different clinical specimens after standard biochemical and microbiological tests. Disc agar diffusion test was applied for antibiotic-resistant examinations. Phenotypic detection of ESBL-producing isolates was performed using mixed disk method. The presence of blaTEM gene was investigated in ESBL-producing isolates using PCR method. The ESBL test analysis was positive for 40 isolates (40%) of K. pneumoniae. The prevalence of blaTEM gene in ESBL-producing K. pneumoniae isolates was 55%. Tetracycline, tobramycin, and ampicillin were the most active antibiotics against K. pneumoniae isolates, showing 85%, 81% and 73% sensitivity, respectively. The highest antibiotic resistance in isolated K. pneumoniae was found for ceftriaxone (48%) and cefotaxime (46%) antibiotics. There was a significant correlation between ESBL production and K. pneumoniae isolates resistance to cefotaxime (p = 0.000), ceftazidime (p = 0.001), ciprofloxacin (p = 0.001), tobramycin (p = 0.044), and ceftriaxone (p = 0.000). The prevalence of ESBL-producing K. pneumoniae was high and increasing. The high prevalence of blaTEM gene in these isolates may be a reason for their pathogenesis and multiple-antibiotic resistance. Therefore, there is a need to develop the strategies to manage antibiotic resistance in these isolates.|
|Beta-lactamase؛ BlaTEM gene؛ Klebsiella pneumoniae؛ PCR؛ Phenotypic and genotypic studies|
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