پیوندهای مفید
آمار
| تعداد نشریات | 31 |
| تعداد شماره‌ها | 748 |
| تعداد مقالات | 7,108 |
| تعداد مشاهده مقاله | 10,240,788 |
| تعداد دریافت فایل اصل مقاله | 6,898,332 |
Extended-spectrum beta-lactamases detection and prevalence of blaTEM gene in clinical isolates of Klebsiella pneumoniae from hospitals in North of Iran | ||
| Caspian Journal of Environmental Sciences | ||
| دوره 20، شماره 1، فروردین 2022، صفحه 29-35 اصل مقاله (678.04 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22124/cjes.2022.5389 | ||
| نویسندگان | ||
| Sharareh Sharafkhah1؛ Roghayeh Oskoueiyan* 1؛ Rabeeh Izadi Amoli1؛ Alamara Gholami2 | ||
| 1Department of Biology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran | ||
| 2Department of Biology, Sari Branch, Islamic Azad University, Sari, Iran | ||
| چکیده | ||
| Klebsiella pneumoniae is a member of the Enterobacteriaceae family which plays an important role in creating various infections in the community. These strains are resistant to multiple β-lactam antibiotics due to the production of β-lactamases enzyme (ESBL). The purpose of this study was to assess the phenotypic and genotypic characteristics of ESBL-producing K. pneumoniae strains from clinical specimens in three hospitals in Mazandaran, Iran. In this cross-sectional study, K. pneumoniae samples (N = 100) were identified from different clinical specimens after standard biochemical and microbiological tests. Disc agar diffusion test was applied for antibiotic-resistant examinations. Phenotypic detection of ESBL-producing isolates was performed using mixed disk method. The presence of blaTEM gene was investigated in ESBL-producing isolates using PCR method. The ESBL test analysis was positive for 40 isolates (40%) of K. pneumoniae. The prevalence of blaTEM gene in ESBL-producing K. pneumoniae isolates was 55%. Tetracycline, tobramycin, and ampicillin were the most active antibiotics against K. pneumoniae isolates, showing 85%, 81% and 73% sensitivity, respectively. The highest antibiotic resistance in isolated K. pneumoniae was found for ceftriaxone (48%) and cefotaxime (46%) antibiotics. There was a significant correlation between ESBL production and K. pneumoniae isolates resistance to cefotaxime (p = 0.000), ceftazidime (p = 0.001), ciprofloxacin (p = 0.001), tobramycin (p = 0.044), and ceftriaxone (p = 0.000). The prevalence of ESBL-producing K. pneumoniae was high and increasing. The high prevalence of blaTEM gene in these isolates may be a reason for their pathogenesis and multiple-antibiotic resistance. Therefore, there is a need to develop the strategies to manage antibiotic resistance in these isolates. | ||
| کلیدواژه‌ها | ||
| Beta-lactamase؛ BlaTEM gene؛ Klebsiella pneumoniae؛ PCR؛ Phenotypic and genotypic studies | ||
| مراجع | ||
|
Ahmed, ZB, Ayad, A, Mesli, E, Messai, Y, Bakour, R & Drissi, M 2012, CTX-M-15 extended-spectrum beta-lactamases in Enterobacteriaceae in the intensive care unit of Tlemcen Hospital, Algeria. East Mediterr Health Journal, 18: 382-396.
Bora, A, Hazarika, NK, Shukla, SK, Prasad, KN, Sarma, JB & Ahmed, G 2014, Prevalence of blaTEM , blaSHV and blaCTX-M genes in clinical isolates of Escherichia coli and Klebsiella pneumoniae from Northeast India, Indian Journal of Pathology and Microbiology, 57: 249-254.
Deguchi, T, Yasuda, M, Yokoi, S, Ishida, K, Ito, M, Ishihara, S, Minamidate, K, Harada, Y, Tei, K, Kojima, K, Tamaki, M & Maeda, S 2003, Treatment of uncomplicated gonococcal urethritis by double-dosing of 200 mg cefixime at a 6-h interval, Journal of Infection and Chemotherapy, 9: 35-49.
Dos Santos, DF, Pimenta, FC, Alves, R, Montalvao, ER, Dos Santos, DB & Do Carmo Filho, JR 2008, Extended-spectrum β-lactamases producing Klebsiella pneumoniae isolated in two hospitals in Goiânia/Brazil: detection, prevalence, antimicrobial susceptibility and molecular typing, Brazilian Journal of Microbiology, 39: 608-612.
Giamarellou, H 2010, Multidrug-resistant Gram-negative bacteria: how to treat and for how long. International Journal of Antimicrobial Agents, 36: S50-4.
Keynan, Y & Rubinstein, E 2007, The changing face of Klebsiella pneumoniae infections in the community, International Journal of Antimicrobial Agents, 30: 385-399.
Lagha, N, Abdelouahid, DE, Hassaine, H, Robin, F & R, B 2014, First characterization of CTX-M-15 and DHA-1 β-lactamases among clinical isolates of Klebsiella pneumoniae in Laghouat Hospital, Algeria. African Journal of Microbiology Research, 8: 1221-1227.
Mahmoudi, S, Pourakbari, B, Rahbarimanesh, A, Abdosalehi, MR, Ghadiri, K & Mamishi, S 2019, An Outbreak of ESBL-producing Klebsiella pneumoniae in an Iranian Referral Hospital: Epidemiology and Molecular Typing. Infectious Disorders-Drug Targets, 19: 46-54.
Maleki, N, Tahanasab, Z, Mobasherizadeh, S, Rezaei, A & Faghri, J 2018, Prevalence of CTX-M and TEM β-lactamases in Klebsiella pneumoniae isolates from patients with urinary tract infection, Al-Zahra Hospital, Isfahan, Iran, Journal of Advanced Biomedical Research, 30: 10-17.
Moini, AS, Soltani, B, Taghavi, Ardakani, A, Moravveji, A, Erami, M, Haji Rezaei, M & Namazi, M 2015, Multidrug-Resistant Escherichia coli and Klebsiella pneumoniae isolated from patients in Kashan, Iran, Jundishapur Journal of Microbiology, 8, e27517.
Parveen, RM, Khan, MA, Menezes, GA, Harish, BN, Parija, SC & Hays, JP 2011, Extended-spectrum β-lactamase producing Klebsiella pneumoniae from blood cultures in Puducherry, India. Indian Journal of Medical Research, 134: 392-395.
Ramazanzadeh, R, Rouhi, S, Shakib, P, Shahbazi, B, Bidarpour, F & Karimi, M 2015, Molecular Characterization of Vibrio cholerae Isolated From Clinical Samples in Kurdistan Province, Iran. Jundishapur Journal of Microbiology, 8: e18119.
Read, PJ, Limnios, EA, Mcnulty, A, Whiley, D & Lahra, MM 2013, One confirmed and one suspected case of pharyngeal gonorrhoea treatment failure following 500 mg ceftriaxone in Sydney, Australia. Sex Health, 10: 460-472.
Rezaee, MA, Sheikhalizadeh, V & Hasani, A 2011, Detection of integrons among multi-drug resistant (MDR) Escherichia coli strains isolated from clinical specimens in northern west of Iran. Brazilian Journal of Microbiology, 42: 1308-1313.
Sarojamma, V & Ramakrishna, V 2011, Prevalence of ESBL-producing Klebsiella pneumoniae isolates in Tertiary Care Hospital. ISRN Microbiology, 2011, 318348.
Shakib, P, Ramazanzadeh, R, Taherikalani, M & Nouri, B 2018, Detection of Extended-Spectrum Beta-Lactamases (ESBLs) and Antibiotic Susceptibility Patterns in Klebsiella pneumoniae in Western Iran. Infectious Disorders- Drug Targets, 18: 156-163.
Taneja, J, Mishra, B, Thakur, A, Dogra, V & Loomba, P 2010, Nosocomial blood-stream infections from extended-spectrum-beta-lactamase-producing Escherichia coli and Klebsiella pneumonia from GB Pant Hospital, New Delhi. Journal of Infection in Developing Countries, 4(8): 517-620.
Zhang, J, Zhou K, ZHENG, B, Zhao, L, Shen, P, JI, J, Wei, Z, Li, L, Zhou, J & Xiao, Y 2016, High Prevalence of ESBL-Producing Klebsiella pneumoniae causing community-onset infections in China. Frontiers Microbiology, 7: 1830. | ||
|
آمار تعداد مشاهده مقاله: 1,509 تعداد دریافت فایل اصل مقاله: 1,318 |
||