|تعداد مشاهده مقاله||7,209,753|
|تعداد دریافت فایل اصل مقاله||5,601,458|
Antagonistic effect of the promising fungal producer strain of microbiopreparation T-1 Trichoderma sp. on oil flax Fusarium blight
|Caspian Journal of Environmental Sciences|
|دوره 19، شماره 5، اسفند 2021، صفحه 883-890 اصل مقاله (1.57 M)|
|نوع مقاله: Research Paper|
|شناسه دیجیتال (DOI): 10.22124/cjes.2021.5248|
|Lyubov Vasilievna Maslienko* ؛ Aliya Khizbullaevna Voronkova؛ Lyubov Anatolyevna Datsenko؛ Evgeniya Alekseevna Efimtseva|
|V.S. Pustovoit All-Russian Research Institute of Oil Crops, 17 Filatova str., Krasnodar, 350038, Russia|
|Fusarium blight is the most harmful disease of oil flax, which affects crops in all cultivation regions during the growing season. Considering the value of flaxseed oil, which is also used for medical purposes, it is advisable to use biological preparations to obtain environmentally safe products, which allow not only to ensure the protection of plants from diseases but also to improve the ecological situation. In recent years (2018-2020), at the biomethod laboratory of V.S. Pustovoit All-Russian Research Institute of Oil Crops has been carried out the search of the promising producer strains for developing microbiopreparations against the pathogen of oil flax Fusarium blight with a multifunctional type of action and a prolonged shelf life. These studies involve the study of the antagonistic mechanisms of promising producer strains of microbiopreparations on the disease pathogen. The article presents the effect of the promising antagonist strain T-1 Trichoderma sp. on the pathogen of oil flax Fusarium blight Fusarium oxysporum Schlecht. emend. Shyd. et Hans. var. orthoceras (App. et Wr.) Bilai, identified as a result of a gradual screening. High competition of the antagonist strain for the feeding area, its hyperparasitic and antibiotic activity was established during the co-cultivation of the antagonist and the pathogen by the method of double cultures. During the study of the mechanism of the antagonistic action of the promising producer strain on the Fusarium blight pathogen using a light microscope, it was found that even before coalescence with the antagonist colony on the second day of coincubation, the shrinking of certain areas in the pathogen mycelium was noted, which proves the antibiotic activity of the fungal antagonist T-1 Trichoderma sp. On the 4th day of co-cultivation, after coalescence of the pathogen and antagonist colonies, aggregation of the pathogen mycelium into the cords and the effect of the antagonist mycelium coiling around the pathogen hyphae were observed . On the 6th day, the effect of the antagonist mycelium coiling around the pathogen hyphae increased. By the 8th-10th days of interaction, there was observed degradation and lysis of the aerial and substrate mycelium of the pathogen of oil flax Fusarium blight. Over the entire observation period, there was noted the normal development of the oil flax Fusarium blight pathogen Fusarium oxysporum var. orthoceras in the control.|
|Oil flax؛ Fusarium blight؛ T-1 Trichoderma sp؛ Antagonist strain|
Abhiram, P & Masih, H 2018, In vitro antagonism of Trichoderma viride against Fusarium oxysporum strains. Journal of Pharmacognosy and Phytochemistry, 7: 2816-2819.
Bilay, VI 1977, Fusaria. Publishing house "Naukova Dumka", Kiev, 440.
Druzhinina, IS, Seidl-Seiboth, V, Herrera-Estrella, A, Horwitz, BA, Kenerley, CM, Monte, E, Mukherjee, PK, Zeilinger, S, Grigoriev, IV & Kubicek, CP 2011, Trichoderma: the genomics of opportunistic success. Nature Reviews Microbiology, 9: 749-759.
Ghazanfar, MU, Raza, M, Raza, W & Qamar, MI 2018, Trichoderma as potential biocontrol agent, its exploitation in agriculture: a review. Plant Protection, 2: 109-135.
Gwa, V & Nwankiti, A 2017, In vitro antagonistic potential of Trichoderma harzianum for biological control of Fusarium moniliforme isolated from Dioscorea rotundata tubers. Virology and Mycology, 6: 2-8.
Jaber, R, Planchon, A, Mathieu-Rivet, E, Kiefer-Meyer, MC, Zahid, A, Plasson, C, Pamlard, O, Beaupierre, S, Trouvé, JP, Guillou, C, Driouich, A, Follet-Gueye, ML & Mollet, JC 2020, Identification of two compounds able to improve flax resistance towards Fusarium oxysporum infection. Plant Science, 301, 110690. [Electronic resource]. https://doi.org/10.1016/j.plantsci.2020.110690.
Karimi, A, Khodaverdiloo, H & Rasouli Sadaghiani, MH 2017, Fungi and bacteria as helping agents for remediation of a Pb - contaminated soil by Onopordum acanthium, Caspian Journal of Environmental Sciences, 15: 249-262
Maslienko, LV 2005, Substantiation and development of a microbiological method to prevent sunflower diseases. PhD Dissertation for Biological Sciences. Krasnodar, 377.
Maslienko, LV, Voronkova AKh, Datsenko LA & Efimtseva, EA 2019, Primary screening of antagonist strains from the collection of the biological method laboratory ARRIOC for the causative agent of oil flax fusarium. Oilseed cultures: Scientific-tech. bul. of ARRIOC, 3: 92-99.
Moradi, M, Matinizadeh, M, Naji, HR, Shirvany, A, Etemad, V, Abdul-Hamid, H & Nazerian, E 2016, Diversity of arbuscular mycorrhizal fungal spores associated with Sorbus torminalis (L.) Crantz. Caspian Journal of Environmental Sciences, 14: 363-371.
Novikova, II 2019, Multifunctional biological products for phytosanitary optimization of agroecosystems in biological agriculture. Technologies and Technical Means of Mechanized Production for Crop and Livestock Products, 2: 183-194.
Nwankiti, A & Gwa, V 2018, Evaluation of antagonistic effect of Trichoderma harzianum against Fusarium oxysporum causal agent of white yam (Dioscorearotundata poir) tuber rot. Trends in Technical & Scientific Research, 1: 12-18.
Pavlyushin, VA 2020, Biological plant protection in greenhouse, intensive and organic farming. BIO Web of Conferences, 18 (89), 00024. – [Electronic resource]. doi: 10.1051 / bioconf / 20201800024
Puyam, A 2016, Advent of Trichoderma as a bio-control agent-a review. Journal of Applied and Natural Science, 8: 1100-1109.
Shahid, M, Srivastava, M, Singh, A, Kumar, V, Rastogi, S, Pathak, N & Srivastava, A 2014, Comparative study of biological agents, Trichoderma harzianum (Th-Azad) and Trichoderma viride (01PP) for controlling wilt disease in pigeon pea. Journal of Microbial & Biochemical Technology, 6: 110-115.
Singh, J, Kumar, V, Srivastava, S, Kumar, A & Singh, VP 2018, In vitro evaluation of Trichoderma species against Fusarium oxysporum f. sp. lycopersici causing tomato wilt. Plant Pathology Journal, 17: 59-64.
Sood, M, Kapoor, D, Kumar, V, Sheteiwy, MS, Ramakrishnan, M, Landi, M, Araniti, F & Sharma, A 2020, Trichoderma: The ‘‘secrets” of a multitalented biocontrol agent. Plants, 9: 762.
Yassin, MT, Mostafa, AAF, Al-Askar, AA, Sayed, Sh. R.M. & Rady, AM 2021, Antagonistic activity of Trichoderma harzianum and Trichoderma viride strains against some fusarial pathogens causing stalk rot disease of maize, in vitro. Journal of King Saud University, Science 33, 101363.
Zhang, F, Yuan, J, Yang, X, Cui, Y, Chen, L, Ran, W & Shen, Q 2013, Putative Trichoderma harzianum mutant promotes cucumber growth by enhanced production of indole acetic acid and plant colonization. Plant Soil, 368: 433-444.
تعداد مشاهده مقاله: 524
تعداد دریافت فایل اصل مقاله: 809