پیوندهای مفید
آمار
| تعداد نشریات | 31 |
| تعداد شماره‌ها | 748 |
| تعداد مقالات | 7,112 |
| تعداد مشاهده مقاله | 10,245,895 |
| تعداد دریافت فایل اصل مقاله | 6,899,568 |
The biochemical and molecular markers of Cyprenus carpio L. after chronic exposure to lead | ||
| Caspian Journal of Environmental Sciences | ||
| دوره 19، شماره 5، اسفند 2021، صفحه 817-824 اصل مقاله (733.67 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22124/cjes.2021.5222 | ||
| نویسندگان | ||
| Wathiq Mohammed* ؛ Suad Ghali Kadhim Alahmed؛ Ayad M.J. Al-mamoori | ||
| Biology Dept. College of Science, University of Babylon, Iraq | ||
| چکیده | ||
| This study was designed to detect the effects of lead on biochemical and molecular characteristics of common carp, Cyprenus carpio L. Two concentrations (10 and 20 ppm) were selected for lead respectively in one week. Biochemical markers such as acetylcholinesterase, superoxide dismutase and catalase were used to assess lead exposure cytotoxic effects, while lead-induced DNA damage was used to determine the effect of lead on molecular level. Fish samples were treated with two selected concentrations of lead solution (10 and 20 ppm) for one-week duration, as exposure period, to detect the impacts of lead exposure on this species. Various biochemical markers such as acetylcholinesterase, superoxide dismutase, and catalase were applied to determine the cytotoxic impacts of lead exposure at the cellular level, while the lead-induced DNA damages were identified to reveal the influences of lead exposure at the molecular level. The results represent that the highest concentration of lead solution (20 ppm) had more effects on the antioxidant enzymes activities such as superoxide dismutase (SOD) level (655.17 ± 21.76 mg U-1) followed by acetyl cholinesterase and catalase (655.17 ± 21.76 U L-1 and 87.93 ± 7.22 mg U-1) respectively. The DNA damages were estimated by the Comet assay technique and the highest level of DNA damages were documented when lead concentration was 20 ppm using different parameters of Comet assay technique such as Comet length, tail length, and tail moment (4021 ± 56.11 µL, 523 ± 55.80 µL, and 91.208 ± 9.45 µL respectively). Therefore, this study confirms that the exposure to high lead concentrations would cause harmful effects on aquatic organisms at both cellular and molecular levels. | ||
| کلیدواژه‌ها | ||
| Biochemical Markers؛ Molecular markers؛ Heavy metals؛ Common carp | ||
| مراجع | ||
|
Alizadeh, S & Mirarab-Razi, J 2016, Growth and accumulation responses of Populus nigra L. exposed to hexavalent chromium excess', Caspian Journal of Environmental Sciences, 14: 253-261.
Bat, L, Yesim Özkan, E, Can Öztekin, H 2015, The contamination status of trace metals in Sinop coast of the Black Sea, Turkey. Caspian Journal of Environmental Sciences, 13: 1-10.
Claiborne, A 1985, Catalase activity. In: CRC handbook of methods for oxygen radical research, RA, Greenwald (ed.), Boca Raton, FL, pp. 283-284.
Çok, I, Uluta, OK, Oku luk, Ö, Durmaz, E & Demir, N 2011, Evaluation of DNA Damage in common carp (Cyprinus carpio L.) by Comet assay for determination of possible pollution in Lake Mogan (Ankara). The Scientific World Journal: TSW Environment, 11: 1455-1461. DOI: 10.1100/tsw. 2011.140
Conners, DE 2004, Biomarkers of oxidative stress in freshwater clam (Corbicula Fluminea) as mechanistic tool to evaluate the impairment of stream ecosystem health by lawn care pesticides. Ph.D thesis, The University of Georgia, U.S.A.
Dias, E, Louro, H, Pinto, M, Santos, T, Antunes, P, Susana, P & Maria, JS 2014, Genotoxicity of Microcystin-LR in in vitro and in vivo experimental models. BioMed Research International, 1: 1-9.
Ding, W & Ong, C 2003, Role of oxidative stress and mitochondrial changes in cyanobacteria-induced apoptosis and hepatotoxicity. FEMS Microbiology Letters, 220: 1-7.
Ellman, GLKD, Courtney, V, Andres, Jr, & Featherstone RM 1961, A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology, 7: 88-95.
Ferencz, Á 2010, Identification and expression of the genes of metal-Responsive transcription factor-1 and glutathione peroxidases of common carp. PhD Dissertation, University of Szeged, Hungary.
Ferrari, A, Venturino A & Péchen de D’Angelo, AM 2007, Muscular and brain cholinesterase sensitivities to azinphos methyl and carbaryl in the juvenile rainbow trout, Oncorhynchus mykiss. Comparative Biochemistry and Physiology, 146: 308-313.
Gabryelak, T, Filipiak A & Brichon, G 2000, Effects of zinc on lipids of erythrocytes from carp (Cyprinus carpio L.) acclimated to different temperatures. Comparative Biochemistry and Physiology Part C, 127: 335-343.
Gaudin, J, Huet S, Jarry, G & Fessard, V 2008, In vivo DNA damage induced by the cyanotoxin microcystin-LR: Comparison of intra-peritoneal and oral administrations by use of the comet assay. Mutation Research, 652: 65-71.
Georg, OO, Amaeze, NH, Soghanmu, TO & Otitoloju, AA 2014, Biomarkers responses in tympanotous fuscatus var radula (L) inhibiting an oil-impacted and fire-ravaged mangrove ecosystem, current advance in environmental science. American V-king Scientific Publishing, 2: 101-111.
Hermesz E & Á. Ferencz 2009, Identification of two phospholipid hydroperoxide gluthatione peroxidase (GPX4) genes in carp. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 150: 101-106.
Janbakhsh, S, Hosseini Shekarabi, S, Shamsaie Mergan, M 2018, Nutritional value and heavy metal content of fishmeal from the Southwest Caspian Sea. Caspian Journal of Environmental Sciences, 16: 307-317. DOI: 10.22124/cjes.2018.3200.
Jia, X, Zhang, H & Liu, X 2011, Low levels of cadmium exposure induce DNA damage and oxidative stress in the liver of Oujiang colored common carp Cyprinus carpio var. Color. Fish Physiology and Biochemistry, 37: 97-103.
Johari, S, Sourinejad, I, Asghari, S, Bärsch, N 2015, Toxicity comparison of silver nanoparticles synthesized by physical and chemical methods to tadpole (Rana ridibunda). Caspian Journal of Environmental Sciences, 13: 383-390.
Lankoff, A, Banasik, A & Obe, G 2004, Effect of microcystin LR and cyanobacterial extract from Polish reservoir of drinking water on cell cycle progression, mitotic spindle, and apoptosis in CHO-K1 cells, Toxicology and Applied Pharmacology, 189: 204-213.
Liu, CI, Zhang, S, Li, Y & Zhang, H 2016, Toxic effects of microcystins on the respiratory system. Life Science Journal, 13: 70-73.
Marklund, S & Marklund, G 1974, Involvement of the superoxide anion radical in the autoxidation of Pyrogallol and a convenient assay for superoxide dismutase. European Journal of Biochemistry, 47: 469-474.
Oveysi, M, Jamili, S, Behdani, M, Mashinchian Moradi, A, Sharifpoor, E 2017, Analysis of vitellogenin gene structure in Caspian roach, Rutilus caspicus (Pisces: Cyprinidae) during exposure to Atrazine. Caspian Journal of Environmental Sciences, 15: 309-319. DOI: 10.22124/cjes.2017. 2638.
Piegorsch, WW & Bailer, AJ 2005, Analyzing environmental data. John wiley & Sons Ltd., UK, ISBN: 978-0-470-84836-4.
Prieto, AI, Jos, A, Pichardo, S, Moreno, I & Camean, AM 2006, Differential oxidative stress responses to microcystins LR and RR in intraperitoneally exposed tilapia fish (Oreochromis sp.). Aquatic Toxicology, 77: 314-321.
Rajamanickam V & Muthuswamy, N 2008, Effect of heavy metals induced toxicity on metabolic biomarkers in common carp (Cyprinus carpio L.). Maejo International Journal of Science and Technology, 2: 192-200.
Said, AK, Ferencz, A, Nemcsók, J & Hermesz, E 2010, Expressions of heat shock and metallothionein genes in the heart of common carp (Cyprinus carpio): Effects of temperature shock and heavy metal exposure. Acta Biologica Hungarica, 61: 10-23.
Singh, NP, McCoy, MT, Tice, RR & Schneider, EL 1988, A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research, 175: 184-191.
Tice, RR, Agurell, E, Anderson, D, Burlinson, B, Hartmann, A, Kobayashi, H, Miyamae, Y, Rojas, E, Ryu, JC & Sasaki, YF 2000, Single cell gel/comet assay: Guidelines for in vitro and in vivo genetic toxicology testing. Environmental and Molecular Mutagenesis, 35: 206-221.
Tripathy & Asima 2016, Oxidative stress, reactive oxygen species (ROS) and Antioxidative defense system, with special reference to Fish. International Journal of Current Research in Biosciences and Plant Biology, 3: 79-89.
Yabanli, M, Alparslan, Y, Hasanhocaoglu Yapici, H, Yapici, S & Yozukmaz, A, 2016, Determination of heavy metal content in commercial marine fish hunted From Southeast Aegean Sea (Turkey) and their potential risk for public health. Caspian Journal of Environmental Sciences, 14: 1-13.
Zegura, B, Volcic, M, Lah, TT & Filipic, M 2008, Different sensitivities of human colon adenocarcinoma (CaCo-2), astrocytoma (IPDDC-A2) and lymphoblastoid (NCNC) cell lines to Microcystin-LR induced reactive oxygen species and DNA damage. Toxicon, 52: 518-525. | ||
|
آمار تعداد مشاهده مقاله: 694 تعداد دریافت فایل اصل مقاله: 909 |
||