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The change in heat inactivation of Escherichia coli O157:H7 after entering into the viable but non-culturable state in salted fish, Hypophthalmichthys molitrix | ||
| Caspian Journal of Environmental Sciences | ||
| دوره 19، شماره 4، دی 2021، صفحه 629-637 اصل مقاله (879.87 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22124/cjes.2021.5136 | ||
| نویسندگان | ||
| Mohammad Khezri1؛ Masoud Rezaei* 1؛ Ashraf Mohabbati Mobarez* 2؛ Mehdi Zolfaghari3 | ||
| 1Department of Seafood Science, Faculty of Marine Sciences, Tarbiat Modares University, Noor, Iran | ||
| 2Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran | ||
| 3Fisheries Department, Agriculture and Natural Resource University of Gorgan, Gorgan, Iran | ||
| چکیده | ||
| Many species of non-sporulating bacteria including Escherichia coli can enter into a viable but non-culturable (VBNC) state under stress conditions. In this study, the change in thermal resistance of E. coli O157:H7 after entering into the VBNC state in salted silver carp, Hypophthalmichthys molitrix, was investigated. E. coli O157:H7 was inoculated on the fish control group (TF) and on those fish with 30% NaCl (TF + 30% NaCl) at room temperature. Culturability of bacteria was determined using routine culture and colony counting on Sorbitol MacConkey agar. When bacteria were non-cultivable, the RT-PCR of 16S rRNA gene (including direct extraction and purification of RNA, DNase I treatment for removing DNA contamination, cDNA synthesis and electrophoresis of PCR products of cDNA) was used to detect VBNC E. coli O157:H7. Also, cultivable and VBNC E. coli O157:H7 were individually heat-treated at 55, 62 and 70 °C for 5 min. The samples were cooled and after 24 h, the thermal resistance of bacteria was determined through viability detection using RT-PCR of 16S rRNA gene. The culturability of bacteria was kept in fish treatment but they were non-cultivable in fish under 30% NaCl after 5 days. The positive expression of 16S rRNA in all studied treatments indicated the entering of E. coli O157:H7 into the VBNC state in fish treatment under 30% NaCl. Moreover, the RT-PCR of 16S rRNA gene showed that only VBNC forms of E. coli O157:H7 showed viability at 62 °C for 5 min which indicated the increased resistant of VBNC bacteria to the thermal inactivation. The ability of E. coli O157:H7 to enter into VBNC state in salted fish and also the increase of its thermal resistance suggest that VBNC E. coli O157: H7 can be considered as critical threat to public health and food safety. | ||
| کلیدواژه‌ها | ||
| Escherichia coli O157:H7؛ VBNC state؛ Thermal resistance | ||
| مراجع | ||
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