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الگوی بیان ژنهای میتوکندریایی و کلروپلاستی برگ برنج در پاسخ به تنش آهن | ||
تحقیقات غلات | ||
دوره 10، شماره 3، آذر 1399، صفحه 207-220 اصل مقاله (273.43 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22124/cr.2021.18472.1642 | ||
نویسندگان | ||
مریم پسندیده ارجمند1؛ محمد محسن زاده گلفزانی* 2؛ حبیب اله سمیع زاده لاهیجی3 | ||
1گروه بیوتکنولوژی.دانشکده کشاورزی.دانشگاه گیلان | ||
2دانشگاه گیلان | ||
3دانشگاه گیلان- ژنتیک مولکولی و بیومتری | ||
چکیده | ||
سمیت آهن سبب اکسیدشدن رنگدانههای فتوسنتزی، افزایش رادیکالهای آزاد اکسیژن و سرانجام کاهش رشد و عملکرد در برنج میشود. با توجه به اهمیت و تاثیر کمبود یا مازاد آهن بر رشد گیاهان زراعی، این پژوهش بهمنظور بررسی اثر آهن بر میزان بیان نسبی ژنهای AOX، IN-NDH، EX-NDH، NDH، FTR و NTR در دو ژنوتیپ حساس (IR64) و متحمل (Pokkali) برنج انجام شد. یون آهن در سطوح صفر (شاهد)، ۱۰۰، ۲۵۰، ۴۰۰ و ۵۰۰ میلیگرم در لیتر تحت شرایط هیدروپونیک در محلول یوشیدا اعمال شد. نتایج نشان داد که افزایش میزان آهن محلول غذایی، سبب افزایش میزان آهن محلول در برگ در هر دو ژنوتیپ شد. اعمال سطوح مختلف آهن تفاوت معنیداری در میزان پتاسیم محلول در برگ ژنوتیپ IR64 ایجاد نکرد، اما سبب افزایش میزان پتاسیم نسبت به نمونههای شاهد در ژنوتیپ Pokkali شد. بیان تمامی ژنهای مورد مطالعه در همه سطوح تنش، بهغیر از NDH و IN-NDH در سطوح 250 و 400 و NTR در سطح 500 میلیگرم در لیتر آهن، در ژنوتیپ Pokkali بیشتر از ژنوتیپ IR64 بود. افزایش بیان نسبی ژنهای مورد بررسی در ژنوتیپ Pokkali نسبت به IR64و نیز تفاوت در روند تغییرات بیان ژن در سطوح مختلف آهن ممکن است تاثیر معنیداری بر میزان تحمل گیاه به سمیت ناشی از آهن داشته باشد و افزایش بیان این ژنها به تنهایی یا با همدیگر میتواند عامل مهمی در خنثی سازی تاثیرات مضر انواع گونه فعال اکسیژن باشد. | ||
کلیدواژهها | ||
آلترناتیو اکسیداز؛ بیان نسبی ژن؛ پتاسیم؛ فردوکسین؛ هیدروپونیک | ||
مراجع | ||
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