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بررسی بیان تعدادی از ژن های مرتبط با تحمل به خشکی در برنج (Oryza sativa L.) | ||
تحقیقات غلات | ||
دوره 9، شماره 4، اسفند 1398، صفحه 299-314 اصل مقاله (472.57 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22124/cr.2020.14616.1563 | ||
نویسندگان | ||
محمد محسن زاده گلفزانی1؛ سمانه آقای پور لیمویی2؛ حبیب اله سمیع زاده لاهیجی* 3؛ شاپور عبدالهی4 | ||
1استادیار، گروه بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران | ||
2دانش آموخته کارشناسی ارشد اصلاح نباتات، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران | ||
3استاد، گروه بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران | ||
4محقق، پژوهشکده بیوتکنولوژی شمال کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، رشت، ایران | ||
چکیده | ||
بهمنظور بررسی تأثیر تنش اسمزی ناشی از اعمال ماده پلیاتیلن گلیکول 6000 بر مولفههای جوانهزنی بذر و تعیین ژنوتیپهای متحمل و حساس به خشکی، 21 ژنوتیپ برنج در سه سطح تنش اسمزی (صفر، 5/7- و 15- مگاپاسکال) با آرایش فاکتوریل بر پایه طرح کاملاً تصادفی با سه تکرار مورد مطالعه قرار گرفتند. نتایج نشان داد که تمام ژنوتیپها در صفات مورد بررسی شامل طول ریشهچه، طول ساقهچه، نسبت طول ریشهچه به ساقهچه، وزن تر ریشهچه، وزن خشک ریشهچه، وزن تر ساقهچه و وزن خشک ساقهچه، دارای تفاوت معنیداری در سطوح مختلف تنش اسمزی بودند. اثر ژنوتیپ و برهمکنش ژنوتیپ و تنش اسمزی در تمامی صفات بررسیشده معنیدار بود. بر اساس صفات اندازهگیری شده، لاین 416 و ژنوتیپ IR28 بهترتیب بهعنوان ژنوتیپهای متحمل و حساس به تنش اسمزی انتخاب شدند. پس از استخراج RNA و سنتز cDNA، الگوی بیان چهار ژن واکنشدهنده به تنش خشکی (SOD، FNR، APX وNDH)در تیمارهای صفر، 4، 8، 10 و 24 ساعت تنش دهیدراسیون در مرحله گیاهچهای در دو ژنوتیپ IR28 و لاین 416 با استفاده از تکنیک RealTime PCR مورد بررسی قرار گرفت. نتایج این پژوهش نشان داد که ژنهای واکنشدهنده به خشکی بیان بالاتری در لاین متحمل 416 نسبت به ژنوتیپ حساس IR28 داشتند و بیان همه ژنها بهجز FNR با افزایش تنش، افزایش یافت. همچنین، ژن APX در زمانهای ابتدایی پس از تنش بیان چندانی در هر دو ژنوتیپ نداشت، اما بیشترین میزان بیان خود را در 24 ساعت پس از تنش در ژنوتیپ حساس IR28 نشان داد. | ||
کلیدواژهها | ||
پلی اتیلن گلیکول؛ تنش اسمزی؛ مولفههای جوانهزنی بذر؛ cDNA؛ Real Time PCR | ||
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