تعداد نشریات | 31 |
تعداد شمارهها | 743 |
تعداد مقالات | 7,072 |
تعداد مشاهده مقاله | 10,129,507 |
تعداد دریافت فایل اصل مقاله | 6,851,109 |
Prevalence of SHV gene and antibiotic resistance of extended-spectrum β-lactamase-producing Escherichia coli strains isolated from abattoir wastewater in Mazandaran Province, north of Iran | ||
Caspian Journal of Environmental Sciences | ||
دوره 19، شماره 1، فروردین 2021، صفحه 11-17 اصل مقاله (883.94 K) | ||
نوع مقاله: Research Paper | ||
شناسه دیجیتال (DOI): 10.22124/cjes.2021.4303 | ||
نویسندگان | ||
Narges Tonekabony1؛ Rabeeh Izadi Amoli1؛ Alamara Gholami2؛ Roghayeh Oskoueiyan1 | ||
1Department of Biology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran | ||
2Department of Biology, Sari Branch, Islamic Azad University, Sari, Iran | ||
چکیده | ||
Abattoir wastewater is a major source of pollution burden and life-treating human pathogenic bacteria. The aim of this study was to determine the extended-spectrum β-lactamase-producing (ESBL) producing E. coli isolates and their antibiotic resistances in wastewater samples. In this experimental study, abattoir wastewater samples were collected and identified from 32 different regions in Mazandaran Province, North Iran. Disk agar diffusion test was applied for testing antibiotic resistance. Phenotypic detection of ESBL-producing isolates was performed using combined disk method. The presence of blaSHV gene was investigated using PCR method. The prevalence of E. coli in wastewater samples was 10%. The ESBL test analysis was positive for 14 (93.33%) isolates. The prevalence of blaSHV gene in ESBL-producing E. coli isolates was 93.34%. The highest antibiotic resistances in ESBL-producing E. coli were found concerning to ceftizoxime and gentamicin (100%), nalidixic acid, ceftazidime and ciprofloxacin (93.34%). Nitrofurantoin was the most effective antibiotic against E. coli isolate exhibiting the highest sensitivity (73.34%). In conclusion, the prevalence of ESBL-producing E. coli is high and being increased. The high prevalence of blaSHV gene in these isolates may be a reason for their pathogenesis and ability in transferring the resistance genes to multiple antibiotics. Therefore, there is a need to develop appropriate treatment and safer disposing abattoir wastes in this province. | ||
کلیدواژهها | ||
Escherichia coli؛ blaSHV gene؛ abattoir water؛ antibiotic resistance | ||
مراجع | ||
Adesemoye, AO, Opere, BO & Makinde, SCO 2006, Microbial content of abattoir wastewater and its contaminated soil in Lagos, Nigeria. African Journal of Biotechnology, 5: 1963-1968.
Asadi, M 2016, Antioxidant and antimicrobial activities in the different extracts of Caspian saffron, Crocus caspius Fisch & amp; C. A. Mey. ex Hohen. Caspian Journal of Environmental Sciences, 14: 331-338.
Behrooozi, A, Rahbar, M & J, VY 2010, Frequency of extended spectrum beta-lactamase (ESBLs) producing Escherichia coli and Klebseilla pneumoniae isolated from urine in an Iranian 1000-bed tertiary care hospital. African Journal of Microbiological Research, 4: 881–884.
Chishimba, K, Hang'ombe, BM, Muzandu, K, Mshana, SE, Matee, MI, Nankajima, C & Suzuki, Y 2016, Detection of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli in Market-Ready Chickens in Zambia. International Journal of Microbiology, 2016: 5275724.
Čornejová, T, Venglovsky, J, Gregova, G, Kmetova, M & Kmet, V 2015, Extended spectrum beta-lactamases in Escherichia coli from municipal wastewater. Annals of Agricultural and Environmental Medicine, 22: 447–450.
Diallo, AA, Brugére, H, Kérourédan, M, Dupouy, V, Toutain, PL, Bousquet-Mélou, A, Oswald, E & Bibbal, D 2013, Persistence and prevalence of pathogenic and extended-spectrum beta-lactamase-producing Escherichia coli in municipal wastewater treatment plant receiving slaughterhouse wastewater. Water Research, 47: 4719-29.
Freedman, D & Et, AL 2005, The relation of child-hood BMI to adult adiposity: The Bogalusa Heart Study. Pedia-trics, 115: 22-27.
Gündoğdu, A, Jennison, AV, Smith, HV, Stratton, H & Katouli, M 2013, Extended-spectrum β-lactamase producing Escherichia coli in hospital wastewaters and sewage treatment plants in Queensland, Australia. Canadian Journal of Microbiology, 59: 737-45.
Highsmith, AK & Abshire, RL 1975, Evaluation of a Most-Probable-Number Technique for the Enumeration of Pseudomonas aeruginosa. Applied Microbiology, 30: 596–601.
Jørgensen, SB, Søraas, AV, Arnesen, LS, Leegaard, TM, Sundsfjord, A & Jenum, PA 2017, A comparison of extended spectrum β-lactamase producing Escherichia coli from clinical, recreational water and wastewater samples associated in time and location. PLoS One, 12: e0186576.
Keynan, Y & Rubinstein, E 2007, The changing face of Klebsiella pneumoniae infections in the community. International Journal of Antimicrobial Agents, 30: 385-9.
Lu, PL, Liu, YC, Toho, HS, Lee, YL, Liu, YM, Ho, CM, Huang, CC, Liu, CE, Ko, WC, Wang, JH, Tang, HJ, Yu, KW, Chen, YS, Chuang, YC, Xu, Y, Ni, Y, Chen, YH & Hsueh, PR 2012, Epidemiology and antimicrobial susceptibility profiles of Gram-negative bacteria causing urinary tract infections in the Asia-Pacific region: 2009-2010 results from the study for monitoring antimicrobial resistance trends (SMART). International Journal of Antimicrobial Agents, 40: S37-43.
M, G 2001, Evolution and epidemiology of extended-spectrum beta-lactamases (ESBLs) and ESBL-producing microorganisms. Clinical Microbiology and Infection, 7: 597-608.
Makhdumi, A 2018, Bacterial diversity in south coast of the Caspian Sea: Culture-dependent and culture-independent survey. Caspian Journal of Environmental Sciences, 16: 259-269.
Mansouri, M & R, R 2009, Spread of extended-spectrum beta-lactamase producing Esherichia coli clinical isolates in Sanandaj hospitals. . Journal of Biological Sciences, 9: 362-366.
Onuoha, SC, Okafor, CO, Aduo, BC & Nwaka, FC 2016, Distribution of antibiotic resistant bacteria from abattoir wastes and its receiving waters at Nkwo-ezzamgbo, Ebonyi State, Nigeria. World Journal of Medical Sciences, 13: 242-250.
Picozzi, S, Ricci, C, Gaeta, M, Macchi, A, Dinang, E, Paola, G, Tejada, M, Costa, E, Bozzini, G, Casellto, S & Carmignani, L 2013, Do we really know the prevalence of multi-drug resistant Escherichia coli in the territorial and nosocomial population? Urology Annals, 5: 25-9.
Picozzi, SCM, Casellato, S, Rossini, M, Paola, G, Tejada, M, Costa, E & Carmignani, L 2014, Extended-spectrum beta-lactamase-positive Escherichia coli causing complicated upper urinary tract infection: Urologist should act in time. Urology Annals, 6:107–112.
Ramazanzadeh, R, Rouhi, S, Shakib, P, Shahbazi, B, Bidarpour, F & Karimi, M 2015, Molecular characterization of Vibrio cholerae isolated from clinical samples in Kurdistan Province, Iran. Jundishapur Journal of Microbiology, 8: e18119.
Sudarwanto, MB, Lukman, DW, Purnawaman, T, Latif, H, Pisestyani, H & Sukmawinata, E 2017, Multidrug resistance extended spectrum β-lactamase and AmpC producing Escherichia coli isolated from the environment of Bogor Slaughterhouse, Indonesia. Asian Pacific Journal of Tropical Biomedicine, 7: 708-711.
Tijani, AA, Famotemi, AC, Orji, FA, Abimbola, FB, Adams, FM & Aba, E 2017, Antimicrobial susceptibility of bacteria isolated from abattoir, eatery and hospital effluents. International Journal of Advanced Research in Biological Sciences, 4: 142-156.
Tokajian, S, Moghnieh, R, Salloum, T, Arabaghian, H, Alousi, S, Moussa, J, Abboud, E, Youssef, S & Husni, R 2018, Extended-spectrum β-lactamase-producing Escherichia coli in wastewaters and refugee camp in Lebanon. Future Medicine, 13: 81–95. | ||
آمار تعداد مشاهده مقاله: 1,065 تعداد دریافت فایل اصل مقاله: 1,166 |