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تاثیر پرایمینگ و تنش اسمزی بر جوانهزنی و فعالیت آنزیمهای هیدرولیتیک و چرخهی گلی اکسیلات در بذر ذرت (Zea mays L.) هیبرید سینگل کراس 704 | ||
علوم و تحقیقات بذر ایران | ||
مقاله 6، دوره 6، شماره 1، خرداد 1398، صفحه 67-78 اصل مقاله (1.18 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22124/jms.2019.3588 | ||
نویسندگان | ||
سیده رقیه خاتمی* ؛ محمد صدقی؛ رئوف سیدشریفی | ||
دانشگاه محقق اردبیلی | ||
چکیده | ||
بهمنظور بررسی اثر انواع پرایمینگ بر میزان فعالیت آنزیمهای چرخه گلی اکسیلات و هیدرولیتیک بذر ذرت (هیبرید SC704) تحت تنش اسمزی، آزمایشی در قالب طرح کاملا تصادفی در سال 1393 در دانشکده کشاورزی و منابع طبیعی دانشگاه محقق اردبیلی صورت گرفت. تیمارهای آزمایش، شامل 4 سطح تنش اسمزی ایجاد شده با پلیاتیلن گلایکول (0، 3-، 6- و 9- بار) و 5 سطح پرایمینگ (شاهد، هیدروپرایمینگ، اسموپرایمینگ، هورموپرایمینگ و اسید آسکوربیک) با سه تکرار بودند. نتایج آزمایش نشان داد که اثر متقابل تیمار تنش اسمزی و پرایمینگ بر تمام صفات اندازهگیری شده به جز درصد جوانهزنی و ایزوسیترات لیاز معنیدار بود. در سطح بدون تنش بالاترین سرعت جوانهزنی (556/17بذر در روز) مشاهده شد. بیشترین میزان فعالیت آنزیمهای آمیلاز (43/4 واحد بر میلیگرم پروتئین)، لیپاز (2/73 واحد بر میلیگرم پروتئین) و پروتئاز (3/2071 واحد بر میلیگرم پروتئین) درتیمار هورمون پرایمینگ و سطح بدون تنش به دست آمد. کمترین میزان فعالیت آنزیم آمیلاز (1/3 واحد بر میلیگرم پروتئین) در تیمار اسموپرایمینگ و سطح خشکی 9- بار بود، در حالی که کمترین میزان فعالیت آنزیمهای لیپاز (36/60 واحد بر میلیگرم پروتئین) و پروتئاز (1949 واحد بر میلیگرم پروتئین) در تیمار پرایمینگ شاهد و سطح خشکی 9- بار مشاهده شد. بالاترین میزان فعالیت مالات سنتاز (7/2 واحد بر میلیگرم پروتئین) در تیمار هورمون پرایمینگ و خشکی شاهد و کمترین آن (88/1 واحد بر میلیگرم پروتئین) در خشکی 9- بار در پرایمینگ شاهد به دست آمد. با افزایش شدت تنش، سرعت جوانهزنی بذر و فعالیت آنزیمها کاهش یافت. در شرایط تنش، پرایمینگ بذر با استفاده از اسید جیبرلیک تا حد زیادی موجب تحمل و جلوگیری از کاهش فعالیت آنزیمهای هیدرولیتیک و چرخهی گلی اکسیلات در بذر ذرت گردید. | ||
کلیدواژهها | ||
ذرت؛ سرعت جوانهزنی؛ آنزیمهای هیدرولیتیک؛ چرخهی گلی اکسیلات | ||
مراجع | ||
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