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تأثیر 8 هفته تمرین مقاومتی فزآینده بر بیان پروتئینهای مؤثر در لیپولیز بافت عضلۀ اسکلتی موشهای صحرایی تغذیه شده با محلول ساکاروز | ||
سوخت و ساز و فعالیت ورزشی | ||
مقاله 2، دوره 7، شماره 1، اردیبهشت 1396، صفحه 21-37 اصل مقاله (1.18 M) | ||
نوع مقاله: مقاله پژوهشی Released under (CC BY-NC) license I Open Access I | ||
شناسه دیجیتال (DOI): 10.22124/jme.2017.3372 | ||
نویسندگان | ||
آرش خدامرادی* 1؛ الهه طالبی گرکانی2؛ فرشته میر محمد رضایی3؛ رزیتا فتحی2 | ||
1دانشجوی دکتری بیوشیمی و متابولیسم ورزشی | ||
2دانشیار دانشگاه مازندران | ||
3استادیار دانشگاه مازندران | ||
چکیده | ||
هدف: انرژی مازاد بدن به صورت تریآسیلگلیسرول (TAG) در بافت چربی اندامهای مختلف ذخیره میشود. به منظور کاهش ذخایر چربی، باید استفاده از آنها برای تولید انرژی افزایش یابد. هدف پژوهش حاضر، بررسی تأثیر 8 هفته تمرین مقاومتی فزآینده و مصرف محلول ساکاروز بر بیان پروتئینهای موثر در لیپولیز بافت عضلۀ اسکلتی موشهای صحرایی بود. روششناسی: 32 سر موش صحرایی نر بالغ از نژاد ویستار (۶-۸ هفتهای)، ابتدا به طور تصادفی به 2 گروه تغذیه شده با و بدون مصرف محلول ساکاروز 30 درصد تقسیم و پس از 4 هفته هر یک از گروهها به دو گروه تمرین و کنترل تقسیم شدند. گروههای تمرینی به مدت 8 هفته تمرین مقاومتی به روش فزآینده (بالا رفتن از نردبان) را 3 روز در هفته انجام دادند. دو روز پس از آخرین جلسه تمرینی نمونه برداری انجام شد. مقدار پروتئین لیپاز حساس به هورمون (HSL)، گیرندهی کبدی ایکس- آلفا (LXRα) و پریلیپین عضلۀ خم کننده بزرگ انگشتان (FHL) به روش وسترن بلات اندازهگیری شد. جهت تعیین معنادار بودن تفاوت بین متغیرها و تعامل بین آنها از تحلیل واریانس دوطرفه در سطح معنیداری 05/0 P<استفاده شد. یافتهها: سطوح پروتئین HSL و پریلیپین در آزمودنیهای گروه تمرین در مقایسه با کنترل افزایش (01/0P<)، اما سطح LXRα بدون تغییر بود. تأثیر مصرف محلول ساکاروز به تنهایی و همچنین تعامل بین مصرف محلول ساکاروز و تمرین مقاومتی فزآینده بر بیان پروتئینهای مذکور در بافت عضلۀ معنادار نبود. نتیجهگیری: نتایج این پژوهش بیانگر آن است که اگرچه 8 هفته تمرین مقاومتی فزآینده موجب افزایش معنادار بیان برخی از پروتئینهای درگیر در لیپولیز شد، لیکن این تغییرات وابسته به مصرف محلول ساکاروز نمیباشد. | ||
کلیدواژهها | ||
واژگان کلیدی: تمرین مقاومتی فزآینده؛ لیپولیز؛ لیپاز حساس به هورمون؛ پری لیپین؛ گیرندۀ x کبدی-آلفا | ||
مراجع | ||
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