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Effects of xenostrogen treatment (4-Nonylphenol, Bisphenol A) on vitellogenin expression in juvenile Cyprinus carpio | ||
Caspian Journal of Environmental Sciences | ||
مقاله 4، دوره 17، شماره 1، خرداد 2019، صفحه 43-53 اصل مقاله (762.76 K) | ||
نوع مقاله: Research Paper | ||
شناسه دیجیتال (DOI): 10.22124/cjes.2019.3343 | ||
نویسندگان | ||
Samar Mortazavi* 1؛ Ali Reza Riyahi Bakhtiari2؛ Abbas Esmaili Sari2؛ Fatemeh Rahbarizadeh3؛ Nasrin Hassanzadeh1 | ||
1Department of Environmental Sciences, Faculty of Natural Resource and Environment, Malayer University, Hamedan, Iran | ||
2Department of Environmental Sciences, Faculty of Natural Resource and Marine Science, Tarbiat Modares University, Noor, Mazandaran, Iran | ||
3Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran | ||
چکیده | ||
The aim of this study was to examine some potential effects of 4-Nonylphenol (4-NP), Bisphenol A (BPA), and mixture of them on common carp, Cyprinus carpio and demonstrate induction of vitellogenin (Vtg) as a biomarker for screening aquatic ecosystems. These compounds with other estrogenic chemicals may be responsible for disrupting effects observed in fish. A total of 100 juvenile carp were treated experimentally with 17β-estradiol (E2), and increasing doses of 4-NP, BPA and also mixture of them. Then the plasma Vtg levels were measured using indirect competitive ELISA. Results showed a significant (P<0.05) increase in Vtg levels of exposed carps with E2, maximum 4-NP, BPA and their mixture. The group treated with E2 showed high induction (490%) with respect to controls. The groups treated with maximum dose of 4-NP and BPA showed also induction of Vtg, whereas the group treated with their mixture (3 mg kg−1 b.w. 4-NP and 0.01 mg kg−1 b.w. BPA) showed the highest induction (2126%). Finally, results showed that mixture of 4-NP and BPA exhibited stronger (synergistic) effects. So that, large scale monitoring of estrogenic effects caused by contamination may be considered as a biomarker in urban and industrial effluents. | ||
کلیدواژهها | ||
Vitellogenin (Vtg)؛ Cyprinus carpio؛ 17β-Esteradiol (E2)؛ 4-Nonylphenol (4-NP)؛ Bisphenol A (BPA)؛ ELISA | ||
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