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تاثیر نانو دیاکسید تیتانیوم و نیترو پروساید سدیم بر شاخصهای جوانهزنی بذر، بنیه و آنزیمهای آنتیاکسیدانت گیاهچهی جو افضل تحت تنش شوری | ||
علوم و تحقیقات بذر ایران | ||
مقاله 4، دوره 5، شماره 3، مهر 1397، صفحه 47-61 اصل مقاله (1.64 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22124/jms.2018.2934 | ||
نویسندگان | ||
افشین کرمی1؛ علی سپهری* 2 | ||
1دانشجوی دکتری، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی دانشگاه بوعلی سینا | ||
2عضو هیات علمی گروه زراعت و اصلاح نباتات دانشکده کشاورزی دانشگاه بوعلی سینا | ||
چکیده | ||
شوری از مهمترین عوامل محدود کننده رشد و عملکرد گیاهان زراعی در بسیاری از مناطق دنیا است. به منظور بررسی اثر نانو دیاکسید تیتانیوم و نیترو پروساید سدیم بر شاخصهای جوانهزنی و میزان آنزیمهای آنتیاکسیدانت گیاهچههای جو رقم افضل تحت تنش شوری آزمایشی به صورت فاکتوریل در قالب طرح کاملاً تصادفی با چهار تکرار انجام شد. فاکتورهای مورد مطالعه شامل 1. نانو دیاکسید تیتانیوم در چهار سطح (0، 500، 1000 و 2000 میلیگرم در لیتر)، 2. نیترو پروساید سدیم در دو سطح (0 و 100 میکرومولار) و 3. تنش شوری در سه سطح (0، 50 و 100 میلیمولار کلرید سدیم) بودند. تنش شوری شاخصهای جوانهزنی بذر مانند درصد و سرعت جوانهزنی، شاخص بنیه، طول ریشهچه، طول ساقهچه، میانگین زمان جوانهزنی و محتوی رطوبت نسبی برگ در گیاهچه را کاهش و نشت الکترولیتی غشاء را افزایش داد. در حضور نانو دیاکسید تیتانیوم اکثر شاخصهای جوانهزنی و فعالیت آنزیمهای آنتیاکسیدانت کاتالاز (CAT)، آسکوربات پراکسیداز (APX) و به ویژه سوپر اکسید دیسموتاز (SOD) افزایش یافتند. کاربرد نیترو پروساید سدیم موجب تعدیل نشت الکترولیتی غشاء (EL) و اثرات منفی تنش شوری شد. برهمکنش نانو دی اکسید تیتانیوم (500 میلیگرم در لیتر) و نیترو پروساید سدیم (100 میکرومولار)، میزان آنزیمهای سوپر اکسید دیسموتاز، آسکوربات پراکسیداز و کاتالاز را تحت تنش 100 میلیمولار کلرید سدیم به ترتیب 28، 8/16 و 9/17 درصد افزایش داد. به نظر میرسد نیترو پروساید سدیم در حضور نانو دیاکسید تیتانیوم از طریق تعدیل اثرات بازدارنده کلرید سدیم، بر بهبود شاخصهای جوانهزنی و میزان آنزیمهای آنتی اکسیدانت گیاهچههای جو تاثیر مناسب داشته باشد. | ||
کلیدواژهها | ||
تخفیفدهنده تنش؛ کلریدسدیم؛ نانو ذره | ||
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