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مطالعه خصوصیات جوانهزنی، بیوشیمیایی و آنزیمی گندمسیاه (Fagopyrum esculentum Moench) تحت تأثیر تنش خشکی و شوری | ||
علوم و تحقیقات بذر ایران | ||
مقاله 3، دوره 5، شماره 3، مهر 1397، صفحه 33-46 اصل مقاله (1.59 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22124/jms.2018.2933 | ||
نویسندگان | ||
مهدی عقیقی شاهوردی* 1؛ آرزو پراور1؛ مجتبی قاسم زاده2؛ عاطفه نوابی3 | ||
1دانشگاه شاهد | ||
2شیراز | ||
3دانشگاه علوم پزشکی اراک | ||
چکیده | ||
به منظور بررسی تأثیر تنش خشکی و شوری بر خصوصیات جوانهزنی، بیوشیمیایی و آنزیمی گندمسیاه دو آزمایش جداگانه در آزمایشگاه علوم و تکنولوژی بذر دانشگاه شاهد در سال 1395 انجام شد. آزمایشها در قالب طرح کاملاً تصادفی در سه تکرار اجرا شد که تیمارها شامل هشت سطح پتانسیل اسمزی (صفر، 2-، 4-، 6-، 8-،10-، 12- و 14- بار) خشکی و شوری بود. نتایج تجزیه واریانس معنیدار بودن اثر تنش خشکی و شوری را بر درصد و سرعت جوانهزنی، طول گیاهچه، بنیه بذر، محتوای کلروفیل، پروتئین محلول، فعالیت آنزیم کاتالاز و سوپر اکسید دیسموتاز نشان داد. نتایج آزمایش تنش شوری و خشکی بر پارامترهای اندازهگیری شده نشان داد که اثرات تیمار شوری و خشکی بازدارنده است اما بازدارندگی تنش اسمزی شوری بیشتر از تنش خشکی بود، در نتیجه، عدم جوانهزنی نرمال در تنش شوری، از 10- بار و در تنش خشکی از 14- بار اتفاق افتاد. از طرفی کاهش محتوای کلروفیل و پروتئین در شرایط تنش خشکی بیشتر از تنش شوری بود و شرایط به وجود آمده منجر به افزایش فعالیت آنزیم کاتالاز در شرایط تنش خشکی و سوپراکسید دیسموتاز در شرایط تنش شوری شد. بنابراین نتایج نشان داد گندمسیاه به تنش خشکی اعمال شده سازگاری و مقاومت بیشتری نسبت به تنش شوری دارد. | ||
کلیدواژهها | ||
پروتئین؛ جوانهزنی؛ سوپر اکسید دیسموتاز؛ کاتالاز؛ کلروفیل؛ گندمسیاه | ||
مراجع | ||
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