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تولید پروتئین هیدرولیز شده از ماهی کیلکا با روش هیدرولیز آنزیمی و سنجش خواص زیستفعالی آن | ||
فیزیولوژی و بیوتکنولوژی آبزیان | ||
مقاله 3، دوره 5، شماره 3، آبان 1396، صفحه 39-58 اصل مقاله (963.82 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22124/japb.2017.2565 | ||
نویسندگان | ||
زهرا نحوی1؛ سید فخرالدین حسینی* 2؛ مژگان زندی3 | ||
1کارشناس ارشد فرآوری محصولات شیلاتی، گروه فرآوری محصولات شیلاتی، دانشکده علوم دریایی، دانشگاه تربیت مدرس، نور، ایران | ||
2استادیار گروه فرآوری محصولات شیلاتی، دانشکده علوم دریایی، دانشگاه تربیت مدرس، نور، ایران | ||
3دانشیار گروه پلیمرهای زیستسازگار، پژوهشگاه پلیمر و پتروشیمی ایران، تهران، ایران | ||
چکیده | ||
در مطالعه حاضر، ابتدا با استفاده از آنزیم آلکالاز از ماهی کیلکای معمولی (Clupeonella cultriventris) پروتئین هیدرولیز شده (FPH) به دست آمد. سپس فعالیت آنتیاکسیدانی پروتئین هیدرولیز شده ماهی به وسیله آزمونهای قدرت مهارکنندگی رادیکالهای آزاد DPPH و ABTS، قدرت کاهندگی آهن، فعالیت شلاته کنندگی یون آهن و فعالیت آنتیاکسیدانی کل در 5 غلظت متفاوت (1، 2، 3، 4 و 5 میلیگرم در میلیلیتر)، مورد ارزیابی قرارگرفت. نتایج بیان کننده آن بود که با افزایش غلظت پروتئین، خاصیت آنتیاکسیدانی FPH به طور معنیداری افزایش یافت. بالاترین فعالیت مهارکنندگی رادیکال DPPH، قدرت کاهندگی آهن، شلاته کنندگی یون آهن و فعالیت آنتیاکسیدانی کل مربوط به غلظت 5 میلیگرم بر میلیلیتر بود. در مجموع میتوان گفت هیدرولیز آنزیمی ماهی کیلکا منجر به تولید پروتئینهایی با خواص آنتیاکسیدانی میشود که میتوانند بعد از تایید مطالعات کلینیکی به عنوان افزودنی مواد غذایی مورد استفاده قرار گیرند. | ||
کلیدواژهها | ||
کیلکای معمولی؛ پروتئین هیدرولیز شده ماهی؛ فعالیت آنتیکسیدانی | ||
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