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اثر تزریق درون آمنیوتیکی روی - متیونین و نانو روی - متیونین بر بیان ژن Zn-T1 و فعالیت آنزیم‌های آلکالین فسفاتاز و مالتاز روده کوچک جوجه‌های گوشتی | ||
| تحقیقات تولیدات دامی | ||
| مقاله 7، دوره 6، شماره 1، خرداد 1396، صفحه 73-87 اصل مقاله (1.01 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22124/ar.2017.2222 | ||
| نویسندگان | ||
| کلثوم رازانی1؛ مجید متقی طلب* 2؛ سید حسین حسینی مقدم3 | ||
| 1دانشجوی دکتری گروه علوم دامی، دانشکده علوم کشاورزی، دانشگاه گیلان | ||
| 2دانشیار گروه علوم دامی، دانشکده علوم کشاورزی، دانشگاه گیلان | ||
| 3استادیار گروه علوم دامی، دانشکده علوم کشاورزی، دانشگاه گیلان | ||
| چکیده | ||
| هدف از این مطالعه بررسی اثر تزریق درونآمنیوتیکی روی - متیونین و نانو روی - متیونین بر بیان ژن انتقالدهنده روی (Zn-T1) و فعالیت آنزیم‌های آلکالین فسفاتاز و مالتاز در ژژنوم جوجه‌های گوشتی بود. چهارصد عدد تخم مرغ بارور به چهار گروه (تیمار)، هریک با چهار زیر گروه (تکرار) تقسیم شد. تیمار اول تا سوم به ترتیبت شامل تزریق درونآمنیوتیکی یک میلیلیتر محلول سرم فیزیولوژی، سرم فیزیولوژی حاوی ۲۵% روی - متیونین و ۲۵% نانو روی - متیونین در روز هفدهم جوجهکشی بود. چهارمین تیمار (کنترل منفی) هیچ تزریقی دریافت نکرد. درصد تفریخ هر تیمار محاسبه و در روزهای اول، سوم و هفتم پرورش، دو پرنده از هر تکرار ذبح شد و بیان ژن Zn-T1، فعالیت آنزیم‌های آلکالین فسفاتاز و مالتاز در بافت روده اندازه‌گیری شد. وزن جوجه‌ها در زمان تفریخ در تیمار روی - متیونین (۰۶/۰±۰۲/۴۳) و نانو روی - متیونین (۰۶/۰±۷۷/۴۲) از سایر تیمارها بیشتر بود (۰۱/۰P˂). همچنین وزن روده کوچک در روزهای یک و هفت پس از تفریخ در تیمار روی - متیونین (به ترتیب ۰۴۸/۰±۷/۹ ، ۰۷/۰±۵۵/۱) و نانو روی - متیونین (به ترتیب ۰۴۸/۰±۵/۹ ، ۰۷/۰±۵۱/۱) از سایر تیمارها بیشتر بود (۰۱/۰P˂). تیمار نانو روی - متیونین در روزهای یک، سه و هفت بعد از تفریخ، بیشترین فعالیت آنزیم آلکالین فسفاتاز (به ترتیب ۱/۰±۸/۱۷، ۰۸/۰±۵/۱۱، ۰۴/۰±۲/۷) مالتاز (به ترتیب ۰۶/۰±۸/۸ ،۰۲/۰±۵/۵ ،۰۳/۰±۲/۴) و بیان ژن Zn-T1 (به ترتیب ۰۰۹/۰±۱۷/۳ ،۰۰۷/۰±۲۴/۴ ،۰۰۵/۰±۶۷/۳) را نشان داد (۰۱/۰P˂). استنتاج نهایی این است که تزریق درون تخم‌مرغی نانو روی - متیونین منجر به افزایش بیان Zn-T1 شده و فعالیت آنزیم‌های آلکالین فسفاتاز و مالتاز روده را در هفته اول پرورش افزایش می‌دهد. | ||
| کلیدواژه‌ها | ||
| آلکالین فسفاتاز؛ جوجه گوشتی؛ مالتاز؛ ناقل روی؛ RT- PCR | ||
| مراجع | ||
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