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Histopathological effects of water soluble?fraction of crude oil on liver tissue of fingerling beluga , Huso huso Linnaeus, 1754 | ||
| Caspian Journal of Environmental Sciences | ||
| مقاله 7، دوره 12، شماره 1، فروردین 2014، صفحه 63-72 اصل مقاله (402.61 K) | ||
| نویسندگان | ||
| al et1؛ E. Khodabakhsh* 2 | ||
| 10 | ||
| 2E. 1*, Sh. Jamili 1, 2, A. Motalebi 2, A. Mashinchian moradi 1, H. Nasrolahzade Saravi 3 | ||
| چکیده | ||
| In this study the LC50 96h values of water-soluble fractions (WSF) of the Caspian Sea ? exploited crude oil were determined in beluga, Huso huso fingerlings. The fingerlings averaged 2.42 ? 0.11 g in weight were exposed to WSF crude oil at concentrations 24.75, 27, 29.25, 31.5, 33.75, 36, 38.25 ppm. The treatments were performed in three replicates and all changes in the specimens were determined for each concentration. Water quality parameters of the seawater were tested and recorded. The LC50 96h value was found to be 34.87 ppm. In the other step, the fingerling beluga was exposed to three sub-lethal concentrations (13.93, 20.92, 27.90 ppm) of WSF of the Caspian crude oil representing 40, 60 and 80% of LC50 96h respectively, and control without crude oil. All treatments and control were conducted in triplicate. Three specimens of each treatment were sampled for histological studies every day. Results revealed that the fish liver tissue of all the treatments showed histological changes, in comparison to the control after 2 days. Even if the changes in the treatment exposed to 27.90 ppm (80% LC50 96h) WSF occurred in 24h after exposure. Histopathological findings in liver included cloudy swelling, pyknotic nuclei, Karyorrhectic nuclei, fatty vacuolation, fatty degeneration, hypertrophy of hepatocyte, necrosis, hypertrophy of bile ducts, cholestasis and bile stagnation, inflammation, fibrosis and cirrosis. These alterations were more conspicuous with increasing the soluble fraction of crude oil concentrations and exposing time. The results are of importance since much attention has been paid to the oil slicks, particulate or sedimentary pollutions, instead of potential toxic effects of water-soluble fractions of oil, which are more available to marine biota. | ||
| کلیدواژهها | ||
| Beluga؛ Huso huso؛ WSF of crude oil؛ Caspian Sea؛ Histology | ||
| مراجع | ||
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Akaishi, F.M., Assis, H.C.S., Jakobi, S.C.G., Eiras-Stofella, D.R., St-Jean .S.D., Courtenay, S.C. 2004. Morphological and neurotoxicological findings in tropical fresh water fish (Astyanax sp.) after waterborne and acute exposure to water soluble fraction (WSF) of crude oil. Archive of Environmental Contamination Toxicology; 46:244–53. Anderson, J.W., Neef, J.M., Cox, B.A., Tatem, H.E., Hightower, G.M.,1974. Characteristics of dispersions and water-soluble extracts of crude and refined oils and their toxicity to estuarine crustaceans and fish. Marine Biology;27:75–88. Anyakora, C., Ogbech, A, Palmer, P., Coker, H., 2005. Determination of poly nuclerar aromatic hydrocarbons in marine samples of siokolo Fishing settlement. Journal of Chromatography A, 1073(1-2): 323-30. Bagherzadeh Lakani, F., Sattari, M., Sharifpour, I., Kazemi, M., 2013. Effect of hypoxia, normoxia and hyperoxia conditions on gill histopathology in Histopathological effects of water...70 two weight groups of beluga (Huso huso) Caspian Journal of Environmental Sciences Vol. 11 No.1 pp. 77~84 Bernet, D., Schmidt, H., Meier, W., Burkhardt- Holm, P, Wahi, T., 1999. Histopathology in fish: proposal for a protocol to assess aquatic pollution. Journal of Fish Disease, 22: 25-34. Binaii, M., Ghiasi, M., Farabi, S.M.V., Pourgholam, R., Fazli, H., Safari, R., Alavi, S.E., Taghavi, M.J., Bankehsaz, Z., 2013. Biochemical and hemato-immunologicalparameters in juvenile beluga (Huso huso) following the diet supplemented with nettle (Urtica dioica). Fish & Shellfish Immunology, In press, Available online 24 October 2013.Brand, D. G., Fink, R., Bengeyfield, W, Birtwell, I. K., Mcallister, C, D., 2001. Salt water-acclimated pink salmon fry (Oncorhynchus gorbuscha) develop stress-related visceral lesions after 10-day exposure to sub-lethal concentration of water –soluble fraction of north slope crude oil. Toxicological Pathology, 29(5): 574-84. Di Toro , D. M., Allen, H, E., Bergman, H. L., MEYER, J. S., Paquin, P. R., Santore, R. C., 2001. Biotic ligand model of the acute toxicity of metals. 1. Technical basis. Environmental Toxicology and Chemistry, 20: 2383-96. Chang, S., Zdanowicz, V. S., Murchelano, R. A., 1998. Associations between liver lesions in winter flounder( Pleuronectes americanus) and sediment chemical contaminants from north-east United states estuaries. ICES,journal of marine Science, 55: 954-969. Engelhardt, F.R., 1983. Petroleum effects on marine mammals. Aquatic Toxicology 4, 199–217. Fanta, E., Rios, F.S., Vianna, A.C.C., Freiberger, S., 2003. Histopathology of the fish corydoras paleatus contaminated with sub-lethal levels of organophosphorusin water and food. Exotoxicology and Environmental Safety, 54: 119-130. Gravato , C., Santos, M. A., 2002. Juvenile Sea Bass Liver P450, EROD Induction, and Erythrocytic Genotoxic Responses to PAH and PAH-like Compounds, Ecotoxicology and Environmental safety, Volum51, Issue 2, 115-127. Haaparanta , A., Tellervo Valtonen, E., Rudolf Hoffmann, R., Holmes, J., 1996. Do macrophage centres in freshwater fishes reflect the differences in water quality?, Aquatic Toxicology, Volume 34, Issue 3, 253-272 . Hoong, G., Huda, K., Lim, W., Thalich, P., 2001. An oil spill-food chain intraction Model for Coastal water. Marine Pollut Bulletin, 42: 590-597. Khan, R.A.,2003. Influence of petroleum at a refinery terminal on witer flounder,(Pleuronectes americanus), Bulletin of Environmental Contamination Toxicology 61, 770-770. Khan, R.A., Ryan, P., 1991. Long term effects of crude oil on common murres (Uria aalge) following rehabilitation. Bulletin of Environmental Contamination Toxicology 46, 216–222. Lockhart , W. L., Duncan , D. V., Billeck , B.N., Danell , R. A., Ryan , M. J., 1996. Chronic toxicity of the ‘water-soluble fraction’ of Norman Wells crude oil to juvenile fish, Spill science & Technology Bulletin, Volume 3, Issue 4, 259-262. Marchand, M. J., van Dyk, J. C., Pieterse, G. M., Barnhoorn, I. E. J., Bornman, M. S., 2008. Histopathological alterations in the liver of the sharptooth catfish Clarias gariepinus from polluted aquatic systems in South Africa, Environmental Toxicology, Volume 24, Issue 2, pages 133–147. Mohamed, F.A.S., 2009. Histopathological studies on Tilapia zillii and Solea vulgaris from Lake Qarun, Egypt. World Journal of Fish Marine Sciences, 1: 29-39. NAP-national academies press., 2003. Oil in the sea III: inputs, fates, andeffects. Washington D.C: National Research Council (U.S.); 278pp. Oliveira Ribeiro, C. A., Belger, L., Pelletier, E., Rouleau , C., 2002. Khodabakhsh et al., 71Histopathological evidence of inorganic mercury and methyl mercury toxicity in the arctic charr (Salvelinus alpinus), Environmental Research, Vol: 90 (2002) 217–22. Pacheco, M. & Santos, A.M. 2001. Biotransformation, endocrine,and genetic responses of Anguilla anguillaL. to petroleum distillate products and environmentally contaminated waters. Ecotoxicology and Environmental Safety, 49: 64-75. Paris- Palacos, S., Biagianti-Risbourg, S. & Vernet, G. 2000. Biochemical and (ultra) structural hepatic perturbation of Brachydanio rerio (Teleostei, Cyprinidae) exposed to two sub-lethal concentrations of copper sulphate. Aquatic. Toxicology. 50:109-124. Rodrigues, E.L., Fanta, E., 1998. Liver histopathology of the fish Brachydanio rerio after acute exposure to sub-lethal levels of the organophosphate Dimetoato 500. Revista Brasileria de Zoologia. 15, 441–450. Saeed T, Al-Mutairi M.1999. Chemical composition of the water-soluble fraction of leaded gasolines in sea water. Environment International; 25:117–29. Sikkema, J., De Bont, J.A., Poolman, B.,1994. Intractions of cyclic hydrocarbons with biological memberance, Journal of Biological Chemistry, 269: 8022-8. Simonata, J.; Guedes, C. and Martinez, C. 2008. Biochemical, physiological and histological changes in neotropical fish Prochildus lineatus exposed to diesel oil. Ecotoxicological Environment. Safety., 69:112-120 . Spies , R.B. Stegman, J. J. Hinton, D. E. Woodin, B. Smolowitz, R. Okihiro, M. Shea, D. 1996. Biomarkers of hydrocarbon exposure and subtidal effect in embiotocide fishes from a natural petroleum seep in the Santa Barbara channel. Aquatic Toxicology 34, 195-514. Stephens, S.M., Brown, J.A., Franklin, S.C., 1997. Stress responses of larval turbot, Scophthalmus maximus L., exposed to sub-lethal concentrations of petroleum hydrocarbons. Fish Physiology and Biochemistry;17:433–9. Traven, F., 1992. Frontier orbitals and properties of organic molecules. Ellis Harwood Publisher, London, p, 235-238. Van der Oost, R., Beber, J., Vermeulen, N.P.E., 2003. Fish bioaccumulation and biomarkers in environmental risk assessment: a review. Environmental Toxicology and Pharmacology, 13: 57- 149. Westernhagen, H., 1989. Sub-lethal effects of pollutants on fish eggs and larvae.In: Hoar W, Randall D, editors. Fish physiology. San Diego: AcademicPress; p. 253–345. | ||
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