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Antimicrobial activity of natural compounds against multidrug-resistant Staphylococcus aureus and Pseudomonas aeruginosa: Effects on viability and resistance expression | ||
| Caspian Journal of Environmental Sciences | ||
| دوره 24، شماره 2، تیر 2026، صفحه 343-351 اصل مقاله (731.36 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22124/cjes.2026.9619 | ||
| نویسندگان | ||
| Gulmira Kyzdarbekova1؛ Anna Kornilova* 2؛ Saule Bazarbaeva* 2؛ Elmira Shamshualieva1؛ Marina Kuznetsova2؛ Aiganym Kazhibayeva1؛ Marat Tynykulov3؛ Botagoz Sharipova1 | ||
| 1Sh. Ualikhanov Kokshetau University, Pedagogical Institute, Department of Biology and teaching methods, Kokshetau, Kazakhstan | ||
| 2M. Kozybaev North Kazakhstan State University, Faculty of Natural sciences, Department of Biology, Petropavlovsk, Kazakhstan | ||
| 3L.N. Gumilyov Eurasian National University, Departments Biotechnology and Microbiology, Astana, Kazakhstan | ||
| چکیده | ||
| Multidrug‑resistant Staphylococcus aureus and Pseudomonas aeruginosa have become a serious problem in Kazakh hospitals, and new treatment options are urgently needed. We investigated whether five locally collected plant extracts could inhibit clinical MDR isolates and suppress the expression of key resistance genes. The work was performed between January and July 2025 in Almaty, Kazakhstan, using 15 S. aureus (10 MRSA) and 15 P. aeruginosa (7 carbapenem‑resistant) isolates. Minimum inhibitory concentrations (MICs) were determined by broth microdilution. Subinhibitory (½ MIC) exposure was followed by quantitative real‑time PCR to measure mecA (MRSA), mexB and ampC (P. aeruginosa) transcripts. Thymus serpyllum (wild thyme) showed the strongest activity, with MIC₅₀ of 16 µg mL-1 against MRSA and 32 µg mL-1 against carbapenem‑resistant P. aeruginosa. No cross‑resistance was observed: MICs did not differ significantly between MRSA and methicillin‑susceptible strains (p = 0.34). At half the MIC, T. serpyllum reduced mecA expression by 59% (mean fold change 0.41, 95% CI 0.36–0.46, p < 0.001). For P. aeruginosa, mexB and ampC transcripts fell to 0.61‑fold (95% CI 0.54–0.68) and 0.71‑fold (0.64–0.78), respectively (both p < 0.001). Suppression was more pronounced in carbapenem‑resistant isolates than in susceptible ones. None of the tested extracts caused upregulation of resistance genes. Glycyrrhiza uralensis was inactive against P. aeruginosa (MIC > 512 µg mL-1). These results demonstrate that T. serpyllum is not only a direct inhibitor of MDR pathogens but also a potent modifier of resistance gene expression. Further studies should identify the active compounds and test combinations with conventional antibiotics. | ||
| کلیدواژهها | ||
| Thymus serpyllum؛ Multidrug resistant bacteria؛ Resistance gene suppression؛ mecA expression | ||
| مراجع | ||
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Bi, Y, Xia, G, Shi, C, Wan, J, Liu, L, Chen, Y, Wu, Y, Zhang, W, Zhou, M, He, H & Liu, M 2021, Therapeutic strategies against bacterial biofilms. Fundamental Research, 1(2): 193–212, https://doi.org/ 10.1016/j.fmre.2021.02.003.
Bonvegna, M, Grego, E, Sona, B, Stella, MC, Nebbia, P, Mannelli, A et al. 2021, Occurrence of methicillin-resistant coagulase-negative staphylococci (MRCoNS) and methicillin-resistant Staphylococcus aureus (MRSA) from pigs and farm environment in northwestern Italy. Antibiotics, 10(6): 676.
Chenouf, NS, Mama, OM, Messaï, CR, Ruiz-Ripa, L, Fernández-Fernández, R, Carvalho, I et al. 2021, Detection of methicillin-resistant coagulase-negative staphylococci and PVL/mecA genes in cefoxitin-susceptible Staphylococcus aureus (t044/ST80) from unpasteurized milk sold in stores in Djelfa, Algeria. Journal of Dairy Science, 104: 2684–2692.
Chenouf, NS, Mama, OM, Messaï, CR, Ruiz-Ripa, L, Fernández-Fernández, R, Carvalho, I et al. 2021, Detection of methicillin-resistant coagulase-negative staphylococci and PVL/mecA genes in cefoxitin-susceptible Staphylococcus aureus (t044/ST80) from unpasteurized milk sold in stores in Djelfa, Algeria. Journal of Dairy Science, 104, 2684-2692.
Ghousepeer, GD, Rani, M, Kumar, A, Singh, SK, Priyadarshini, A, Leal, E & Pandey, RP 2026, Insights into drug resistance in Leishmania: Mechanisms, therapeutics, and clinical case studies. ADMET and DMPK, 14: 2992, https://doi.org/10.5599/admet.2992.
Grazul, M, Balcerczak, E & Sienkiewicz, M 2023, Analysis of the presence of the virulence and regulation genes from Staphylococcus aureus (S. aureus) in coagulase negative staphylococci and the influence of the staphylococcal cross-talk on their functions. International Journal of Environmental Research and Public Health, 20, 5155.
Hamza, AA, Albassam, T, AbdulHussein, AH, Ghazy, H & Dadaxon, A 2025, Fig leaves as waste-derived activated carbons for effective absorption of amoxicillin drug: Isotherm and regeneration study. Procedia Environmental Science, Engineering and Management, 12 (2): 485-494.
Hamid, S, Taha, A & Hussien, S 2025, The emerging threat of multidrug-resistant mecA gene-positive coagulase-negative Staphylococci. Iranian Journal of Microbiology, 17(4): 577–585.
Hirakawa, H, Kurushima, J, Hashimoto, Y & Tomita, H 2020, Progress overview of bacterial two-component regulatory systems as potential targets for antimicrobial chemotherapy. Antibiotics, 9(10): 635. https://doi.org/10.3390/antibiotics9100635.
Ibragimova, F, Kalenderova, G, Kalmuratova, B, Okhunova, M, Kholmuradova, Z, Ikramova, F & Axmedov, N 2025, The association between gut microbiota-derived metabolites and arterial stiffness in hypertensive patients. Revista Latinoamericana de Hipertensión, 20(11): 818-824, http://doi.org/ 10.5281/zenodo.17925018.
Jednačak, T, Stepanić, V, Habinovec, I, Mikulandra, I, Smokrović, K, Čipčić Paljetak, H & Novak, P 2026, Anti-infective macrozones: design, biological evaluation and structure-activity relationships, ADMET and DMPK, 14: 3139, https://doi.org/10.5599/admet.3139.
Kadhim, R, Al-Mosawi, R & Manji, Z 2024, Synthesis of silver nanoparticles using medicinal plant extracts for antimicrobial usages. Procedia Environmental Science, Engineering and Management, 11(2): 285-296.
Medis, S, Dissanayake, T, Weerasekera, M, Namali, D, Gunasekara, S & Kottahachchi, J 2022, Distribution of mecA and qacA/B genes among coagulase negative staphylococci isolated from central venous catheters of intensive care unit patients. Indian Journal of Medical Microbiology, 40: 505–509.
Murray, CJ, Ikuta, KS, Sharara, F, Swetschinski, L, Robles Aguilar, G, Gray, A, Naghavi, M 2022, Global burden of bacterial antimicrobial resistance in 2019: A systematic analysis. The Lancet, 399(10325): 629–655, https://doi.org/10.1016/S0140-6736(21)02724-0.
Naghavi, M, Vollset, SE, Ikuta, KS, Swetschinski, LR, Gray, AP, Wool, EE, Dolecek, C 2024, Global burden of bacterial antimicrobial resistance 1990–2021: A systematic analysis with forecasts to 2050. The Lancet, 404(10456): 1199–1226, https://doi.org/10.1016/S0140-6736(24)01867-1.
Nasaj, M, Saeidi, Z, Asghari, B, Roshanaei, G & Arabestani, MR 2020, Identification of hemolysin encoding genes and their association with antimicrobial resistance pattern among clinical isolates of coagulase-negative Staphylococci. BMC Research Notes, 13: 68.
Shrestha, LB, Bhattarai, NR, Rai, K & Khanal, B 2020, Antibiotic resistance and mecA gene characterization of coagulase-negative staphylococci isolated from clinical samples in Nepal. Infection and Drug Resistance, 13: 3163-3169.
Shikhranova, DD, Belyavskaya, AD, Gubina, AA, Gennadievna, GA, Mikhailyuk, LM, Barinova, ES & Sokolova, AE 2024, Medicine and comprehensive treatment of hypertension: drug and non-drug therapy. Revista Latinoamericana de Hipertension, 19(10): 413-421.
Wang, D, Ning, Q, Deng, Z, Zhang, M & You, J 2022, Role of environmental stresses in elevating resistance mutations in bacteria: Phenomena and mechanisms. Environmental Pollution, 307: 119603, https://doi.org/10.1016/j.envpol.2022.119603.
Wang, Y & Lam, ATW 2020, Epigallocatechin gallate and gallic acid affect colonization of abiotic surfaces by oral bacteria. Archives of Oral Biology, 120: 104922, https://doi.org/10.1016/j.archoralbio. 2020.104922.
Wulandari, DA, Zein, MIHL, Zakiyyah, SN, Ishmayana, S, Ozsoz, M, Hartati, YW & Irkham, I 2025, CRISPR-Cas9-based electrochemical biosensor for the detection of katG gene mutations in isoniazid-resistant tuberculosis. ADMET and DMPK, 13(3): 2766, https://doi.org/10.5599/admet.2766.
Zhang, L, Liang, E, Cheng, Y, Mahmood, T, Ge, F, Zhou, K, Liu, L 2020, Is combined medication with natural medicine a promising therapy for bacterial biofilm infection? Biomedicine & Pharmacotherapy, 128: 110184, https://doi.org/10.1016/j.biopha.2020.110184.
Zhu, Y, Huang, WE & Yang, Q 2022, Clinical perspective of antimicrobial resistance in bacteria. Infection and Drug Resistance, 15: 735–746, https://doi.org/10.2147/IDR.S345574. | ||
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