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Effects of cadmium chloride on the ultrastructure of animal somatic cells (gums, kidneys, and liver) | ||
| Caspian Journal of Environmental Sciences | ||
| دوره 24، شماره 1، فروردین 2026، صفحه 99-106 اصل مقاله (838.95 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22124/cjes.2026.9501 | ||
| نویسندگان | ||
| Zina B. Tungushbaeva* 1؛ Rakhat K. Pernebekova* 2؛ Nurzhamal M. Joldasbayeva3؛ Gabit Sharipov4؛ Sarzhan Sharipova5؛ Zannym M. Yermentayeva6؛ Indira Z. Kaketaeva6؛ Kuanysh Syman3 | ||
| 1Head of the Department of Molecular Biology, Kazakh-Russian Medical University, Almaty, Kazakhstan | ||
| 2Department of Pharmacology, Pharmacotherapy and Clinical Pharmacology, Faculty of Pharmacy, South Kazakhstan Medical Academy, Shymkent, Kazakhstan | ||
| 3Department of Biology, Faculty of Natural Sciences and Geography, Abai Kazakh National Pedagogical University, Almaty, Kazakhstan | ||
| 4Department of Civil Defense and Military Training, Malik Gabdullin Academy of Civil Protection of the Ministry of Emergency Situations of the Republic of Kazakhstan, Kokshetau, Kazakhstan | ||
| 5Department of Toxicological Chemistry, Asfendiyarov Kazakh National Medical University, Almaty, Kazakhstan | ||
| 6Head of the Department of Anatomy with courses, Kazakh-Russian Medical University, Almaty, Kazakhstan | ||
| چکیده | ||
| Cadmium is a toxic heavy metal with the ability to accumulate in various organs of the body that can cause severe cellular damage. This study was conducted to investigate and compare the microstructural changes caused by cadmium chloride in three important somatic tissues including gums, kidney and liver in an animal model. In this experimental study, 30 male rats were divided into three groups: control, low dose (2 mg kg-1) and high dose (5 mg kg-1) of cadmium chloride for 28 days. After the end of the period, tissue samples were collected and examined using transmission electron microscopy. Qualitative changes were recorded and quantitative parameters including the percentage of swollen mitochondria, nuclear chromatin condensation index and the number of cytoplasmic vacuoles were measured by ImageJ software and analyzed by appropriate statistical tests. The results of the study confirmed severe and dose-dependent damage in all tissues. The percentage of swollen mitochondria in hepatocytes increased from 2.4% in the control group to 3.72% in the high-dose group, a significant increase (p < 0.05). The liver showed the highest sensitivity, followed by the kidney and then the gingiva. The chromatin condensation index in gingival cells at the high dose reached 2.7, indicating significant nuclear changes in this tissue. A strong dose-response relationship was observed for all measured parameters (Pearson correlation r = 0.98 for liver mitochondrial damage, p < 0.001). The formation of cytoplasmic vacuoles was also significantly increased in the treatment groups. Consequently, cadmium chloride causes significant microstructural damage at the cellular level, the pattern of which varies in different tissues. Mitochondria were identified as the main target of toxicity in all tissues. These findings provide direct and exact evidence of the mechanisms of cadmium cytotoxicity and emphasize the need to manage exposure to this toxic metal. | ||
| کلیدواژهها | ||
| Cadmium chloride؛ Cellular microstructure؛ Tissue toxicity؛ Transmission electron microscopy؛ Oxidative stress | ||
| مراجع | ||
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Arbi, S, Bester, MJ, Pretorius, L & Oberholzer, HM 2021, Adverse cardiovascular effects of exposure to cadmium and mercury alone and in combination on the cardiac tissue and aorta of Sprague-Dawley rats. Journal of Environmental Science and Health, Part A, 56(6): 609-624, https://doi.org/10.1080/10934529.2021.1899534.
Bhattacharjee, B, Pal, PK, Ghosh, AK, Mishra, S, Chattopadhyay, A & Bandyopadhyay, D 2019, Aqueous bark extract of Terminalia arjuna protects against cadmium-induced hepatic and cardiac injuries in male Wistar rats through antioxidative mechanisms. Food and Chemical Toxicology, 124: 249-264, https://doi.org/10.1016/ j.fct.2018.12.008.
Branca, JJV, Fiorillo, C, Carrino, D, Paternostro, F, Taddei, N, Gulisano, M, Pacini, A & Becatti, M 2020, Cadmium-induced oxidative stress: Focus on the central nervous system. Antioxidants, 9(6): 492, https://doi.org/ 10.3390/antiox9060492.
Cheng, CH, Ma, HL, Deng, YQ, Feng, J, Jie, YK & Guo, ZX 2021, Oxidative stress, cell cycle arrest, DNA damage and apoptosis in the mud crab (Scylla paramamosain) induced by cadmium exposure. Chemosphere, 263: 128277, https://doi.org/10.1016/j.chemosphere.2020.128277.
Dong, AG, Ma, YY, Wang, XL, Jing, XJ, He, H, Zhang, TM, Dong, HD, Liu, W, Fan, KF & Huo, JF 2024, Effect of cadmium on histopathological injuries and ultra-structural changes of kidney of the turtle Mauremys reevesii. Environmental Science and Pollution Research, 31(26): 39774-39781, https://doi.org/10.1007/s11356-024-33859-w.
Fang, J, Xie, S, Chen, Z, Wang, F, Chen, K, Zuo, Z & Cui, H 2021, Protective effect of vitamin E on cadmium-induced renal oxidative damage and apoptosis in rats. Biological Trace Element Research, 199(12): 4675-4687, https://doi.org/10.1007/s12011-021-02591-8.
Genchi, G, Sinicropi, MS, Lauria, G, Carocci, A & Catalano, A 2020, The effects of cadmium toxicity. International Journal of Environmental Research and Public Health, 17(11): 3782, https://doi.org/10.3390/ ijerph17113782.
Ghosh, K 2018, Cadmium treatment induces echinocytosis, DNA damage, inflammation, and apoptosis in cardiac tissue of albino Wistar rats. Environmental Toxicology and Pharmacology, 59: 43-52, https://doi.org/10.1016/ j.etap.2018.02.009.
Gobe, G & Crane, D 2010, Mitochondria, reactive oxygen species and cadmium toxicity in the kidney. Toxicology Letters, 198(1): 49-55, https://doi.org/10.1016/j.toxlet.2010.04.013.
Habeebu, SS, Liu, J & Klaassen, CD 1998, Cadmium-induced apoptosis in mouse liver. Toxicology and Applied Pharmacology, 149(2): 203-209, https://doi.org/10.1006/taap.1997.8374.
Hassanin, M, Kerek, EM, Chiu, MH, Antikovskiy, M & Prenner, E J 2016, Binding affinity of inorganic mercury and cadmium to biomimetic erythrocyte membrane. The Journal of Physical Chemistry B, 120(50): 12872–12882, https://doi.org/10.1021/acs.jpcb.6b10542.
Husanov, S, Allaberganov, O, Asadova, N, Abdullayev, X, Matyakubov, M, Ubaydova, D & Shayeva, R 2025, The brain-heart-kidney axis in hypertension: Integrative biomarker discovery and translational implications show. Revista Latinoamericana de Hipertensión, 20(9): 667-674, https://doi.org/10.5281/zenodo.17314379.
Lee, WK & Thévenod, F 2020, Cell organelles as targets of mammalian cadmium toxicity. Archives of Toxicology, 94(4): 1017-1049, https://doi.org/10.1007/s00204-020-02692-8.
Li, H, Fagerberg, B, Sallsten, G, Borné, Y, Hedblad, B, Engström, G & Barregard, L 2019, Smoking-induced risk of future cardiovascular disease is partly mediated by cadmium in tobacco: Malmö Diet and Cancer Cohort Study. Environmental Health, 18(1): 56, https://doi.org/10.1186/s12940-019-0495-1.
Liu, Y, Du, C, Lin, CW, Gao, XM, Zhu, JQ & Zhang, CD 2022, Characterization of copper/zinc superoxide dismutase activity on Phascolosoma esculenta (Sipuncula: Phascolosomatidea) and its protection from oxidative stress induced by cadmium. International Journal of Molecular Sciences, 23(19): 12136, https://doi.org/ 10.3390/ijms231912136.
Messner, B, Türkcan, A, Ploner, C, Laufer, G & Bernhard, D 2016, Cadmium overkill: autophagy, apoptosis and necrosis signalling in endothelial cells exposed to cadmium. Cellular and Molecular Life Sciences, 73(8): 1699-1713, https://doi.org/10.1007/s00018-015-2094-9.
Saleh, RM & Awadin, WF 2017, Biochemical and histopathological changes of subacute cadmium intoxication in male rats. Environmental Science and Pollution Research, 24(32): 25475-25481, https://doi.org/10.1007/ s11356-017-0348-9.
Satarug, S, Vesey, DA & Gobe, GC 2017, Health risk assessment of dietary cadmium intake: Do current guidelines indicate how much is safe? Environmental Health Perspectives, 125(3): 284-288, https://doi.org/ 10.1289/EHP108
Thevenod, F & Lee, WK 2013, Toxicology of cadmium and its damage to mammalian organs. Metal Ions in Life Sciences, 11: 415-490, Springer, https://doi.org/10.1007/978-94-007-5179-8_14.
Tinkov, AA, Filippini, T, Ajsuvakova, OP, Aaseth, J, Gluhcheva, YG, Ivanova, JM, Bjørklund, G, Skalnaya, M G, Gatiatulina, ER, Popova, EV, Nemereshina, ON, Vinceti, M & Skalny, AV 2018, Cadmium and atherosclerosis: A review of toxicological mechanisms and a meta-analysis of epidemiologic studies. Environmental Research, 162: 240-260, https://doi.org/10.1016/j.envres.2018.01.008.
Yan, LJ & Allen, DC 2021, Cadmium-induced kidney injury: Oxidative damage as a unifying mechanism. Biomolecules, 11(11): 1575, https://doi.org/10.3390/biom11111575. | ||
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