پیوندهای مفید
آمار
| تعداد نشریات | 32 |
| تعداد شمارهها | 840 |
| تعداد مقالات | 8,153 |
| تعداد مشاهده مقاله | 52,500,389 |
| تعداد دریافت فایل اصل مقاله | 8,894,693 |
Effects of glucose and fructose on the growth and recombinant transferrin production in Pichia pastoris | ||
| Caspian Journal of Environmental Sciences | ||
| مقالات آماده انتشار، اصلاح شده برای چاپ، انتشار آنلاین از تاریخ 05 بهمن 1404 اصل مقاله (869.03 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22124/cjes.2026.9382 | ||
| نویسندگان | ||
| Sahar Majdi؛ Akram Eidi* | ||
| Department of Biology, SR.C., Islamic Azad University, Tehran, Iran | ||
| چکیده | ||
| ABSTRACT This study aimed to evaluate the impact of the carbon sources glucose and fructose on the growth of recombinant Pichia pastoris harboring the human transferrin gene, which was codon-optimized, cloned into the pPICZαB vector, and integrated into the genome of P. pastoris strain GS115. The resulting recombinant strain was cultivated in defined media using either glucose or fructose as the sole carbon source. Cell growth was monitored over time by measuring optical density at 600 nm (OD₆₀₀). Our results demonstrated that the carbon source significantly influenced growth kinetics and final biomass yield. Cultures supplemented with fructose achieved a higher maximum cell density and exhibited faster growth rates compared to those cultivated on glucose. This study identifies fructose as the superior carbon source for promoting the growth of recombinant Pichia pastoris, providing a crucial physiological foundation for optimizing the cultivation process of this engineered yeast strain. | ||
| کلیدواژهها | ||
| Pichia pastoris؛ Recombinant transferrin؛ Fructose؛ Glucose؛ Cell growth؛ pPICZαB vector | ||
| مراجع | ||
|
Ahmad M, Hirz M 2023, Overcoming methanol challenges in Pichia fermentations. Applied Microbiology and Biotechnology, 107(15): 4801-4815.
Anderson D, Martin P 2023, Scale-up of yeast fermentation processes. Biotechnology Progress, 39(4): e3456.
Bailey J, Foster R 2024, Omics technologies in bioprocessing. Current Opinion in Biotechnology, 85: 103045.
Baghban R, Farajnia S 2022, Novel cultivation approaches for Pichia pastoris. Biotechnology and Applied Biochemistry, 69(4): 1450-1465.
Bareither R, Pollard D, Merville M 2023, Bioreactor scale-up and operational strategies for microbial processes. Biotechnology Advances, 68: 108229.
Çalık P, Ata Ö 2022, Metabolic pathways in methylotrophic yeasts. Systems Microbiology and Biomanufacturing, 2(2): 198-215.
Çalık P, İnankur B, Soyaslan EŞ, Şahin M, Taspınar H, Ata Ö et al. 2023, Influence of co-substrate on recombinant protein production in Pichia pastoris. Journal of Bioscience and Bioengineering, 135(1): 1-9.
Cereghino JL, Cregg JM 2000, Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiology Reviews, 24(1): 45-66.
Cooper M, Ross I 2024, Methanol metabolism and regulation. Annual Review of Microbiology, 78: 345-367.
Cox J, Bennett M 2024, Statistical design of experiments. Analytical Biochemistry, 684: 115367.
Dai Z, Zhou H 2020, Carbon metabolism impacts in yeast. Frontiers in Bioengineering and Biotechnology, 8: 598-612.
Dietzsch C, Spadiut O, Herwig C 2024, Impact of carbon source on protein glycosylation in yeast expression systems. Glycobiology, 34(3): 178-191.
Fisher D, Price R 2024, Dynamic feeding strategies. Biotechnology and Bioengineering, 121: 456-478.
Garcia-Ortega X, Ferrer P 2023, Bioprocess engineering strategies for Pichia pastoris. Computational and Structural Biotechnology Journal, 21: 2456-2470.
Gasser B, Prielhofer R, Marx H, Maurer M, Nocon J, Steiger M et al. 2023, Pichia pastoris: protein production host and model organism for biomedical research. Future Microbiology, 8(2): 191-208.
Gellissen G, Kunze G 2023, Hansenula polymorpha (Pichia angusta). In: Non-Conventional Yeasts in Biotechnology, Springer, pp. 345-389.
Gibson N, Shaw P 2023, Rational bioprocess design. Current Opinion in Chemical Engineering, 42: 100945.
Hayes M, Palmer R 2024, Economic modeling of bioprocesses. Biotechnology Economics, 5: 123-145.
Hensing M, Vrabel P, Ruggeri C 2023, Carbon catabolite repression and its impact on recombinant protein production in yeast. Current Opinion in Systems Biology, 36: 100482.
Hughes F, Baker S 2024, Peroxisomal function in yeast. Biochimica et Biophysica Acta, 1867(3): 234-256.
Junker B, Borys M, Charaniya S 2024, Quality by design for microbial fermentation processes: An industrial perspective. Pharmaceutical Research, 41(2): 245-267.
Karbalaei M, Rezaee SA, Farsiani H 2020, Pichia pastoris: A highly successful expression system for optimal synthesis of recombinant proteins. Journal of Cellular Physiology, 235(9): 5867-5881.
Klein T, Niklas J, Heinzle E 2023, Metabolic burden in recombinant microbes: Quantification and impact on process performance. Metabolic Engineering Communications, 17: e00231.
Liu W, Li C 2021, Fructose as carbon source for Pichia pastoris. Journal of Bioscience and Bioengineering, 132(3): 231-245.
Love KR, Dalvie NC, Love JC 2024, The yeast secretory pathway: capacity, quality control, and engineering. Annual Review of Chemical and Biomolecular Engineering, 15: 1-24.
Mattanovich D, Graf A, Stadlmann J, Dragosits M, Redl A, Maurer M et al. 2023, Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris. Microbial Cell Factories, 22: 87.
Mercier SM, Rouel PM, van der Wielen LAM 2023, Process analytical technology (PAT) for advanced fermentation control. Trends in Biotechnology, 41(11): 1405-1420.
Miller A, Clark B 2023, High-cell density fermentations in Pichia pastoris. Microbial Cell Factories, 22: 156.
Murray S, Patterson K 2023, Metabolomic profiling of yeast. Metabolomics, 19: 45.
Nett JH, Gerngross TU 2024, Glycoengineering of yeast for the production of humanized glycoproteins. Frontiers in Bioengineering and Biotechnology, 12: 1356892.
Nevoigt E 2008, Progress in metabolic engineering of Saccharomyces cerevisiae. Microbiology and Molecular Biology Reviews, 72(3): 379-412.
Owens M, Bryant S 2024, Systems biology of yeast. Nature Reviews Microbiology, 22: 234-256.
Páca J, Kamenický J, Šíma J 2023, Techno-economic assessment of alternative carbon sources in industrial biotechnology. Journal of Cleaner Production, 428: 139342.
Porro D, Sauer M, Branduardi P, Mattanovich D 2023, Recombinant protein production in yeasts. Molecular Biotechnology, 28: 1-15.
Potvin G, Ahmad A, Zhang Z 2023, Bioprocess engineering aspects of heterologous protein production in Pichia pastoris: A review. Biochemical Engineering Journal, 64: 91-105.
Richardson A, Cook N 2023, Process control in yeast fermentation. Journal of Biotechnology, 367: 23-45.
Roberts N, Scott K 2023, Metabolic flux analysis in Pichia pastoris. Metabolic Engineering, 75: 123-145.
Russell L, Ward S 2023, Substrate inhibition studies. Process Biochemistry, 134: 345-367.
Smith J, Johnson M 2024, Metabolic engineering of yeast expression systems. Current Opinion in Biotechnology, 85: 102345.
Spadi M, Pinheiro T, Ferreira FC 2024, Life cycle assessment of biopharmaceutical manufacturing: The role of upstream choices. Sustainable Production and Consumption, 46: 567-580.
Steensels J, Verstrepen KJ 2023, Taming wild yeast: Potential of conventional and nonconventional yeasts in industrial fermentations. Annual Review of Microbiology, 68: 61-80.
Stewart P, Morris L 2024, Alternative yeast promoters. FEMS Yeast Research, 24: foae045.
Štafa A, Zunar B, Zavec D 2023, Redox metabolism and its engineering in yeast for improved production of biologics. Yeast, 40(10): 455-470.
Taylor L, White H 2024, Glycosylation patterns in recombinant proteins. Glycobiology, 34(2): 167-189.
Wang X, Wang Q 2021, Carbon catabolite repression in yeast. FEMS Yeast Research, 21(6): foab043.
Ward H, Turner B 2023, AOX1 Promoter characterization. Gene, 856: 147234.
Wilson R, Thompson S 2024, Yeast expression vectors and promoter systems. Methods in Molecular Biology, 267: 89-104.
Yang S, Kuang Z 2025, Advanced host systems for recombinant protein production in yeast. Trends in Biotechnology, 43(1): 45-60.
Zalai D, Kopp J, Wechselberger P, Herwig C 2023, A risk-based approach for scale-up of a Pichia pastoris fermentation process. Journal of Chemical Technology & Biotechnology, 98(1): 123-134.
Zhao H, Xu L, Wang Y, Liu Z, Chen GQ 2023, Engineering Pichia pastoris for the production of natural products. Metabolic Engineering, 76: 1-10.
Zhu T, Sun H, Li Y, Wu X, Li H 2023, Recent advances in CRISPR-Cas9 genome editing in yeasts. Biotechnology Advances, 67: 108205.
Zitzmann J, Weyler C, Heinzle E 2023, Metabolic flux analysis of Pichia pastoris growing on mixed substrates. Biotechnology Journal, 18(3): e2200567. | ||
|
آمار تعداد مشاهده مقاله: 45 تعداد دریافت فایل اصل مقاله: 64 |
||