پیوندهای مفید
آمار
| تعداد نشریات | 32 |
| تعداد شمارهها | 819 |
| تعداد مقالات | 7,952 |
| تعداد مشاهده مقاله | 40,977,757 |
| تعداد دریافت فایل اصل مقاله | 8,472,218 |
Impacts of Haematococcus pluvialis extract on the expression of the SOX2 and OCT4 genes in the prostate cancer cell line, PC3 | ||
| Caspian Journal of Environmental Sciences | ||
| مقالات آماده انتشار، اصلاح شده برای چاپ، انتشار آنلاین از تاریخ 14 آذر 1404 اصل مقاله (921.53 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22124/cjes.2025.9261 | ||
| نویسندگان | ||
| Mahsa Khatami؛ Fatemeh Moradi؛ Mahmoudreza Aghamaali* ؛ Seyedeh Fatemeh Fallah | ||
| Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran | ||
| چکیده | ||
| There is a small population of cells with stem cell properties in cancer tissues that are responsible for the initiation of tumour formation and have the ability to resist apoptosis inducing agents and chemotherapy. SOX2 is a transcription factor in stem cells and OCT4 is a pluripotency factor known as cancer stem cell markers. The use of plant compounds as cancer suppressors has become increasingly important today. The purpose of this study is to investigate the expression of self-renewal genes SOX2 and OCT4 in a prostate cancer cell line (PC3) treated with different concentrations of methanolic, chloroform, aqueous and hexane extracts of the green microalga Haematococcus pluvialis. PC3 cells were exposed to varying concentrations of microalgae extract after cultivation. The percentage of survival was checked using the MTT method, and the change in expression change of SOX2 and OCT4 genes was studied using the RT-qPCR technique. The results of the evaluations showed that the hexane extract of microalgae caused a significant decrease in the number of living cells compared to the control and significantly decreased the expression of the SOX2 and OCT4 genes. Therefore, the hexane extract of H. pluvialis can be considered a factor affecting the expression pathway in self-renewal genes of stem cells. | ||
| کلیدواژهها | ||
| Prostate cancer؛ SOX2؛ OCT4؛ Green microalgae؛ MTT | ||
| مراجع | ||
|
Bradford, MM 1976, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248-254.
Dere, S, Gunes, T & Sivaci, R 1998, Spectrophotometric determination of chlorophyll-A, B and total carotenoid contents of some algae species using different solvents. Turkish Journal of Botany, 22: 13-18.
Frank, NY, Schatton, T & Frank, MH 2010, The therapeutic promise of the cancer stem cell concept. Journal of Clinical Investigation, 120:41-50.
Gagliardi, A, Mullin, NP, Ying Tan Z, Colby, D, Kousa, AI, Halbritter, F & Chambers I 2013, A direct physical interaction between Nanog and Sox2 regulates embryonic stem cell self‐renewal. EMBO Journal, 32: 2231-2247.
Hosseini, M, Jahani, Y, Mahmoudi, M, Eshraghian, MR, Yahyapour, Y & Keshtkar AA 2008, The assessment of risk factors for prostate cancer in Mazandaran province, Iran. Journal of Guilan University of Medical Sciences, 10:58-64.
Kampa, M, Hatzoglou, A, Notas, G, Damianaki, A, Bakogeorgou, E, Gemetzi, C & Castanas, E 2000, Wine antioxidant polyphenols inhibit the proliferation of human prostate cancer cell lines. Nutrition and Cancer, 37: 223-233.
Kazłowska, K, Lin, HV, Chang, SH & Tsai, GJ 2013, In vitro and in vivo anticancer effects of sterol fraction from red algae Porphyra dentata. Evidence‐Based Complementary and Alternative Medicine, 2013: 493869.
Li, F, Cai, M, Lin, M, Huang, X, Wang, J, Ke, H & Yang, S 2020, Enhanced biomass and astaxanthin production of Haematococcus pluvialis by a cell transformation strategy with optimized initial biomass density. Marine Drugs, 18: 341.
Li, Y, Huang, W, Huang, S, Du, J & Huang, C 2012, Screening of anti-cancer agent using zebrafish: comparison with the MTT assay. Biochemical and biophysical research communications, 422: 85-90.
Mansur, AA, Brown, MT & Billington, RA 2020, The cytotoxic activity of extracts of the brown alga Cystoseira tamariscifolia (Hudson) Papenfuss, against cancer cell lines changes seasonally. Journal of Applied Phycology, 32: 2419-2429.
Meijer, EA & Wijffels, RH 1998, Development of a fast, reproducible and effective method for the extraction and quantification of proteins of micro-algae. Biotechnology Techniques, 12: 353-358.
Moradi, F, Aghamaali, M & Hadavi, M 2023, Osteogenic differentiation of human amniotic mesenchymal stem cells by phycocyanin and phycoerythrin pigments isolated from Spirulina platensis and Gracilaria gracilis algae. Tissue and Cell, 85: 102216.
Moussavou, G, Kwak, DH, Obiang-Obonou, BW, Ogandaga Maranguy, CA, Dinzouna-Boutamba, SD, Lee, DH & Choo, YK 2014, Anticancer effects of different seaweeds on human colon and breast cancers. Marine Drugs, 12: 4898-4911.
Namvar, F, Mohamad, R, Baharara, J, Zafar-Balanejad, S, Fargahi, F & Rahman, HS 2013, Antioxidant, antiproliferative, and antiangiogenesis effects of polyphenol-rich seaweed (Sargassum muticum). BioMed Research International, 2013: 604787.
Narasimhan, MK, Pavithra, SK, Krishnan, V & Chandrasekaran, M 2013, In vitro analysis of antioxidant, antimicrobial and antiproliferative activity of Enteromorpha antenna, Enteromorpha linza and Gracilaria corticata extracts. Jundishapur Journal of Natural Pharmaceutical Products, 8:151.
Nichols, HW 1973, Growth media-freshwater. In: Stein JR., editor. Handbook of phycological methods. Culture methods and measurements. Cambridge University Press, London, pp:7- 24.
Radzisheuskaya, A & Silva, JC 2014, Do all roads lead to Oct4? The emerging concepts of induced pluripotency. Trends in cell biology, 24: 275-284.
Rais, J, Jafri, A, Siddiqui, S, Tripathi, M & Arshad, M 2017, Phytochemicals in the treatment of ovarian cancer. Front Biosci (Elite Ed), 9: 67-75.
Sakthivel, R & Devi, KP 2019, Antioxidant, anti-inflammatory and anticancer potential of natural bioactive compounds from seaweeds. Studies in natural products chemistry, 63:113-160.
Salehi, P, Sonboli, A, Eftekhar, F, Nejad-Ebrahimi, S & Yousefzadi, M 2005, Essential oil composition, antibacterial and antioxidant activity of the oil and various extracts of Ziziphora clinopodioides subsp. rigida (B OISS.) R ECH.f. from Iran. Biological and pharmaceutical bulletin, 28: 1892-1896.
Senthilkumar, K, Manivasagan, P, Venkatesan, J & Kim, SK 2013, Brown seaweed fucoidan: biological activity and apoptosis, growth signaling mechanism in cancer. International journal of biological macromolecules, 60: 366-374.
Shah, MMR, Liang, Y, Cheng, JJ & Daroch, M 2016, Astaxanthin-producing green microalga Haematococcus pluvialis: from single cell to high value commercial products. Frontiers in plant science, 7: 531.
Sotomayor, P, Godoy, A, Smith, GJ & Huss, WJ 2009, Oct4A is expressed by a subpopulation of prostate neuroendocrine cells. The Prostate, 69: 401-410.
Vaddi, PK, Stamnes, MA, Cao, H & Chen, S 2019, Elimination of SOX2/OCT4-associated prostate cancer stem cells blocks tumour development and enhances therapeutic response. Cancers, 11: 1331.
Wang, N, Pei, H, Xiang, W, Li, T, Lin, S, Wu, J & Wu, H 2023, Rapid screening of microalgae as potential sources of natural antioxidants. Foods, 12: 2652.
Wu, K, Ying, K, Zhou, J, Liu, D, Liu, L, Tao, Y & Cai, Z 2021, Optimizing the growth of Haematococcus pluvialis based on a novel microbubble-driven photobioreactor. Iscience, 24: 103461.
Yi, Z, Yin-Shan, C & Hai-Sheng, LU 2001, Screening for antibacterial and antifungal activities in some marine algae from the Fujian coast of China with three different solvents. Chinese journal of Oceanology and Limnology, 19: 327-331.
Zamani, L, Zakir Bostanabad, S & Masoud Salehipour, M 2017, Investigating the effect of spirulina extract on DU145 cell line and OCT4 gene expression in cells treated with the extract. New Journal of Cell-Molecular Biotechnology, 8: 53-63.
Zhang, Q, Han, Z, Zhu, Y, Chen, J & Li, W 2020, The role and specific mechanism of OCT4 in cancer stem cells: a review. International journal of stem cells, 13: 312-325. | ||
|
آمار تعداد مشاهده مقاله: 3 تعداد دریافت فایل اصل مقاله: 4 |
||