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Kanzhigitova, Moldir, Yessimova, Anar, Narymbayeva, Zaure, Mamytova, Assiya, Abubakirova, Azhar, Tashmuxamedov, Mugrajidin, Tashmukhamedova, Sh.S.. (1404). Elisa-based enzyme isolation, purification, and characterization of polygalacturonase and α-amylase from Aspergillus oryzae. فیزیولوژی و بیوتکنولوژی آبزیان, (), 1-15. doi: 10.22124/cjes.2025.8916
Moldir Kanzhigitova; Anar Yessimova; Zaure Narymbayeva; Assiya Mamytova; Azhar Abubakirova; Mugrajidin Tashmuxamedov; Sh.S. Tashmukhamedova. "Elisa-based enzyme isolation, purification, and characterization of polygalacturonase and α-amylase from Aspergillus oryzae". فیزیولوژی و بیوتکنولوژی آبزیان, , , 1404, 1-15. doi: 10.22124/cjes.2025.8916
Kanzhigitova, Moldir, Yessimova, Anar, Narymbayeva, Zaure, Mamytova, Assiya, Abubakirova, Azhar, Tashmuxamedov, Mugrajidin, Tashmukhamedova, Sh.S.. (1404). 'Elisa-based enzyme isolation, purification, and characterization of polygalacturonase and α-amylase from Aspergillus oryzae', فیزیولوژی و بیوتکنولوژی آبزیان, (), pp. 1-15. doi: 10.22124/cjes.2025.8916
Kanzhigitova, Moldir, Yessimova, Anar, Narymbayeva, Zaure, Mamytova, Assiya, Abubakirova, Azhar, Tashmuxamedov, Mugrajidin, Tashmukhamedova, Sh.S.. Elisa-based enzyme isolation, purification, and characterization of polygalacturonase and α-amylase from Aspergillus oryzae. فیزیولوژی و بیوتکنولوژی آبزیان, 1404; (): 1-15. doi: 10.22124/cjes.2025.8916

Elisa-based enzyme isolation, purification, and characterization of polygalacturonase and α-amylase from Aspergillus oryzae

Caspian Journal of Environmental Sciences
مقالات آماده انتشار، اصلاح شده برای چاپ، انتشار آنلاین از تاریخ 31 تیر 1404
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22124/cjes.2025.8916
نویسندگان
Moldir Kanzhigitova* 1؛ Anar Yessimova1؛ Zaure Narymbayeva2؛ Assiya Mamytova2؛ Azhar Abubakirova* 3؛ Mugrajidin Tashmuxamedov4؛ Sh.S. Tashmukhamedova5
1M. Auezov South Kazakhstan University, Shymkent City, Kazakhstan
2Zhumabek Akhmetuly Tashenev University, Shymkent City, Kazakhstan
3Zhanibekov University, Shymkent City, Kazakhstan
4Tashkent Institute of Chemical Technology, Tashkent, 32, Navoi Street, Uzbekistan
5National University of Uzbekistan, named after Mirzo Ulugbek, Tashkent City, Uzbekistan
چکیده
The α-amylase (α-A) and polygalacturonase (PG) enzymes produced by Aspergillus oryzae are the primary subjects of this research, which also aims to isolate, purify, and characterize them. Biological material utilisation was conducted by all applicable ethical norms. To achieve highly pure enzymes with improved activity, the purification process included ammonium sulphate precipitation, dialysis, and size-exclusion chromatography employing Toyopearl HW-65 resin. We used SDS-PAGE and gel filtration to find the molecular weights of α-amylase and polygalacturonase. We tested the enzyme activity under different pH, temperature, and substrate conditions. According to Michaelis-Menten kinetics, substrate-specific activity was found through a kinetic study. α-amylase showed optimal activity at pH 6.8, and polygalacturonase at pH 4.8. Enzyme-linked immunosorbent assays (ELISAs) demonstrated the presence and sensitive quantification of these enzymes. To emphasise their regulatory roles, researchers examined how divalent cations and inhibitors affected enzyme activity. By examining the catalytic properties and operational stability of these enzymes, the study highlights their potential industrial applications in food processing, biotechnology, and diagnostics.
کلیدواژه‌ها
Enzyme-Linked Immuno Sorbent Assay (ELISA)؛ A.Oryzae؛ polygalacturonase؛ α-amylase؛ characterisation
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تعداد مشاهده مقاله: 24


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