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Elisa-based enzyme isolation, purification, and characterization of polygalacturonase and α-amylase from Aspergillus oryzae | ||
| Caspian Journal of Environmental Sciences | ||
| دوره 23، شماره 5، اسفند 2025، صفحه 1271-1282 اصل مقاله (1.05 M) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22124/cjes.2025.8916 | ||
| نویسندگان | ||
| Moldir Kanzhigitova* 1؛ Anar Yessimova1؛ Zaure Narymbayeva2؛ Assiya Mamytova2؛ Azhar Abubakirova* 3؛ Mugrajidin Tashmuxamedov4؛ Sh.S. Tashmukhamedova5 | ||
| 1M. Auezov South Kazakhstan University, Shymkent City, Kazakhstan | ||
| 2Zhumabek Akhmetuly Tashenev University, Shymkent City, Kazakhstan | ||
| 3Zhanibekov University, Shymkent City, Kazakhstan | ||
| 4Tashkent Institute of Chemical Technology, Tashkent, 32, Navoi Street, Uzbekistan | ||
| 5National University of Uzbekistan, named after Mirzo Ulugbek, Tashkent City, Uzbekistan | ||
| چکیده | ||
| The α-amylase (α-A) and polygalacturonase (PG) enzymes produced by Aspergillus oryzae are the primary subjects of this research, which also aims to isolate, purify, and characterize them. Biological material utilisation was conducted by all applicable ethical norms. To achieve highly pure enzymes with improved activity, the purification process included ammonium sulphate precipitation, dialysis, and size-exclusion chromatography employing Toyopearl HW-65 resin. We used SDS-PAGE and gel filtration to find the molecular weights of α-amylase and polygalacturonase. We tested the enzyme activity under different pH, temperature, and substrate conditions. According to Michaelis-Menten kinetics, substrate-specific activity was found through a kinetic study. α-amylase showed optimal activity at pH 6.8, and polygalacturonase at pH 4.8. Enzyme-linked immunosorbent assays (ELISAs) demonstrated the presence and sensitive quantification of these enzymes. To emphasise their regulatory roles, researchers examined how divalent cations and inhibitors affected enzyme activity. By examining the catalytic properties and operational stability of these enzymes, the study highlights their potential industrial applications in food processing, biotechnology, and diagnostics. | ||
| کلیدواژهها | ||
| Enzyme-Linked Immuno Sorbent Assay (ELISA)؛ A.Oryzae؛ polygalacturonase؛ α-amylase؛ characterisation | ||
| مراجع | ||
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